Validation Data Gallery
|Positive WB detected in||HeLa cells, COLO 320 cells, Jurkat cells, mouse colon tissue|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human colon cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:1000-1:4000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
12525-1-AP targets IRF2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, chicken|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IRF2 fusion protein Ag3220|
|Full Name||IRF 2|
|Calculated molecular weight||349 aa, 39 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC015803|
|Gene ID (NCBI)||3660|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
IRF2, also known as IFN regulatory factor 2, which belongs to the IRF family. IRF2 specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the IFN consensus sequence (ICS)) and represses those genes. IRF2 also acts as an activator for several genes including H4 and IL7. IRF2 constitutively binds to the ISRE promoter to activate IL7. IRF2 is involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. The calculated molecular weight of IRF2 is 39 kDa, but post-modification of IRF2 is about 50 kDa (PMID: 17698501).
|Product Specific Protocols|
|WB protocol for IRF2 antibody 12525-1-AP||Download protocol|
|IHC protocol for IRF2 antibody 12525-1-AP||Download protocol|
|IF protocol for IRF2 antibody 12525-1-AP||Download protocol|
|IP protocol for IRF2 antibody 12525-1-AP||Download protocol|
|Click here to view our Standard Protocols|
J Dermatol Sci
J Immunol Res
Propofol Ameliorates Microglia Activation by Targeting MicroRNA-221/222-IRF2 Axis.
Int J Mol Sci
Analysis of miRNA Expression Profiling of RIP2 Knockdown in Chicken HD11 Cells When Infected with Avian Pathogenic E. coli (APEC).
IRF2 inhibits ZIKV replication by promoting FAM111A expression to enhance the host restriction effect of RFC3.