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  • KD/KO Validated

IRF2 Polyclonal antibody

IRF2 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12525-1-AP

Synonyms

IRF 2, IRF2



Tested Applications

Positive WB detected inHeLa cells, COLO 320 cells, Jurkat cells, mouse colon tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

12525-1-AP targets IRF2 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRF2 fusion protein Ag3220
Full Name IRF 2
Calculated molecular weight 349 aa, 39 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC015803
Gene symbol IRF2
Gene ID (NCBI) 3660
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRF2, also known as IFN regulatory factor 2, which belongs to the IRF family. IRF2 specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the IFN consensus sequence (ICS)) and represses those genes. IRF2 also acts as an activator for several genes including H4 and IL7. IRF2 constitutively binds to the ISRE promoter to activate IL7. IRF2 is involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. The calculated molecular weight of IRF2 is 39 kDa, but post-modification of IRF2 is about 50 kDa (PMID: 17698501).

Protocols

Product Specific Protocols
WB protocol for IRF2 antibody 12525-1-APDownload protocol
IHC protocol for IRF2 antibody 12525-1-APDownload protocol
IP protocol for IRF2 antibody 12525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

J Dermatol Sci

PMID: 31138473

Authors - Xiangzhi Li
  • KD Validated
mouseWB

J Immunol Res

Propofol Ameliorates Microglia Activation by Targeting MicroRNA-221/222-IRF2 Axis.

Authors - Xi Xiao
  • KD Validated