IRF2 Polyclonal antibody

• Featured Product
• KD/KO Validated

IRF2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 12525-1-AP

Synonyms

IRF 2, IRF2

---

Validation Data Gallery

• 

  WB analysis using 12525-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12525-1-AP (IRF2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 12525-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12525-1-AP (IRF2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 12525-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 12525-1-AP (IRF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse colon using 12525-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 12525-1-AP (IRF2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 12525-1-AP

  IP result of anti-IRF2 (IP:12525-1-AP, 4ug; Detection:12525-1-AP 1:500) with Jurkat cells lysate 2800ug.

• 

  IHC staining of human colon cancer using 12525-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12525-1-AP (IRF2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 12525-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 12525-1-AP (IRF2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of Jurkat using 12525-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using IRF2 antibody (12525-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: HeLa cells, COLO 320 cells, Jurkat cells, mouse colon tissue
• Positive IP detected in: Jurkat cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Product Information

12525-1-AP targets IRF2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IRF2 fusion protein Ag3220
• Full Name: IRF 2
• Calculated Molecular Weight: 349 aa, 39 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC015803
• Gene Symbol: IRF2
• Gene ID (NCBI): 3660
• RRID: AB_2296127
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

IRF2, also known as IFN regulatory factor 2, which belongs to the IRF family. IRF2 specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the IFN consensus sequence (ICS)) and represses those genes. IRF2 also acts as an activator for several genes including H4 and IL7. IRF2 constitutively binds to the ISRE promoter to activate IL7. IRF2 is involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. The calculated molecular weight of IRF2 is 39 kDa, but post-modification of IRF2 is about 50 kDa (PMID: 17698501).

Publications

Species	Application	Title
chicken	WB	Int J Mol Sci; Analysis of miRNA Expression Profiling of RIP2 Knockdown in Chicken HD11 Cells When Infected with Avian Pathogenic E. coli (APEC).; Authors - Hongyan Sun
human	WB	Virol J; IRF2 inhibits ZIKV replication by promoting FAM111A expression to enhance the host restriction effect of RFC3.; Authors - Kai Ren ; KD Validated
mouse	WB	J Immunol Res; Propofol Ameliorates Microglia Activation by Targeting MicroRNA-221/222-IRF2 Axis.; Authors - Xi Xiao ; KD Validated
human	WB,IHC	J Dermatol Sci; PMID: 31138473; Authors - Xiangzhi Li ; KD Validated
human	WB	Transl Oncol; IRF2-ferroptosis related gene is associated with prognosis and EMT in gliomas; Authors - Shiao Tong ; KD Validated
human	WB	Pathol Res Pract; Long noncoding RNA SENCR facilitates the progression of acute myeloid leukemia through the miR-4731-5p/IRF2 pathway; Authors - Changhao Han