IRF3 Antibody 21 Publications

Rabbit Polyclonal| Catalog number: 11312-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 30 μg/150 μl

Choose size:

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Species specificity:
human, mouse, rat, pig, sheep

Positive WB detected in:
Jurkat cells, human brain tissue, mouse brain tissue, rat brain tissue, U-937 cells

Positive IP detected in:
Jurkat cells

Positive IHC detected in:
human spleen tissue

Positive IF detected in:
HepG2 cells

Positive FC detected in:
HepG2 cells

Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:200
IF : 1:10-1:100

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
interferon regulatory factor 3

Calculated molecular weight:
47 kDa

Observed molecular weight:
49-55 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
IRF3

Synonyms
interferon regulatory factor 3, IRF 3, IRF3
Background

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. IRF3 exists some isoforms with MV 47-49 kDa, 33 kDa and 12-16 kDa.


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