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IRF3 Antibody

IRF3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 11312-1-AP

Synonyms

interferon regulatory factor 3, IRF 3, IRF3



Tested Applications

Positive WB detected inHeLa cells, U-937 cells, human brain tissue, Jurkat cells, mouse brain tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

11312-1-AP targets IRF3 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, pig, rat, sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRF3 fusion protein Ag1858
Full Name interferon regulatory factor 3
Calculated molecular weight 47 kDa
Observed molecular weight 49-55 kDa
GenBank accession number BC009395
Gene symbol IRF3
Gene ID (NCBI) 3661
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. IRF3 exists some isoforms with MV 47-49 kDa, 33 kDa and 12-16 kDa.

Protocols

Product Specific Protocols
WB protocol for IRF3 antibody 11312-1-APDownload protocol
IHC protocol for IRF3 antibody 11312-1-APDownload protocol
IF protocol for IRF3 antibody 11312-1-APDownload protocol
IP protocol for IRF3 antibody 11312-1-APDownload protocol
FC protocol for IRF3 antibody 11312-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Front Immunol

Herpes Simplex Virus Type 2 Immediate Early Protein ICP27 Inhibits IFN-β Production in Mucosal Epithelial Cells by Antagonizing IRF3 Activation.

Authors - Xinmeng Guan
mouseWB

Front Physiol

Evidence for Sexual Dimorphism in the Response to TLR3 Activation in the Developing Neonatal Mouse Brain: A Pilot Study.

Authors - Raul Chavez-Valdez

FASEB J

USP7-TRIM27 axis negatively modulates antiviral type I IFN signaling.

Authors - Juan Cai
humanWB,IF

ACS Infect Dis

The Nonstructural Protein of Guertu Virus Disrupts Host Defenses by Blocking Antiviral Interferon Induction and Action.

Authors - Yuan-Qin Min
pigWB

Microb Pathog

Porcine 2', 5'-oligoadenylate synthetase 2 inhibits porcine reproductive and respiratory syndrome virus replication in vitro.

Authors - Mengmeng Zhao
humanWB

Biochem Biophys Res Commun

Inhibition of cGAS-STING-TBK1 signaling pathway by DP96R of ASFV China 2018/1.

Authors - Xixi Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sarah (Verified Customer) (12-18-2019)

This antibody is awesome- been searching for an Pab IRF 3 antibody that works well in a species other than human, and we finally found this one! Thank you!

  • Applications: Western Blot,
No image submitted with review