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IRF3 Polyclonal antibody
IRF3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 11312-1-AP
|Positive WB detected in||HeLa cells, U-937 cells, human brain tissue, Jurkat cells, mouse brain tissue|
|Positive IP detected in||Jurkat cells|
|Positive IHC detected in||human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
11312-1-AP targets IRF3 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, pig, rat, sheep|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IRF3 fusion protein Ag1858|
|Full Name||interferon regulatory factor 3|
|Calculated molecular weight||47 kDa|
|Observed molecular weight||49-55 kDa|
|GenBank accession number||BC009395|
|Gene ID (NCBI)||3661|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The virul-induced expression of interferon(IFN) genes in infected cells implicate in the interplay of several constitutively expressed and virus-activated transcription factors. A family of IFN regulatory factors(IRFs) have been shown to has a role in the transcription of IFN genes as well as IFN-stimulated genes. IRF3 is a novel key transcriptional regulator of type I IFN-dependent immune responses and involves in the innate immune response against DNA and RNA viruses, by binding to the promoters of IFN. It located in the cytoplasm of uninfected cells in an inactive form, and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, could be phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization, nuclear localization and association with CREB binding protein (CREBBP) to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. IRF3 exists some isoforms with MV 47-49 kDa, 33 kDa and 12-16 kDa.
|Product Specific Protocols|
|WB protocol for IRF3 antibody 11312-1-AP||Download protocol|
|IHC protocol for IRF3 antibody 11312-1-AP||Download protocol|
|IF protocol for IRF3 antibody 11312-1-AP||Download protocol|
|IP protocol for IRF3 antibody 11312-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Herpes Simplex Virus Type 2 Immediate Early Protein ICP27 Inhibits IFN-β Production in Mucosal Epithelial Cells by Antagonizing IRF3 Activation.
Evidence for Sexual Dimorphism in the Response to TLR3 Activation in the Developing Neonatal Mouse Brain: A Pilot Study.
USP7-TRIM27 axis negatively modulates antiviral type I IFN signaling.
ACS Infect Dis
SRP54 Negatively Regulates IFN-Beta Production and Antiviral Response by Targeting RIG-I and MDA5.
A Nuclear Export Signal Is Required for cGAS to Sense Cytosolic DNA.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sarah (Verified Customer) (12-18-2019)
This antibody is awesome- been searching for an Pab IRF 3 antibody that works well in a species other than human, and we finally found this one! Thank you!