IRF7 Polyclonal antibody

IRF7 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22392-1-AP

Synonyms

IRF 7, IRF 7H, IRF7, IRF7A



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 5 publications below

Product Information

The immunogen of 22392-1-AP is IRF7 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, Paralichthys olivaceus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRF7 fusion protein Ag18059
Full Name IRF 7
Calculated molecular weight 516 aa, 56 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC136555
Gene symbol IRF7
Gene ID (NCBI) 3665
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

IRF-7 (IFN regulatory factor 7) is a member of the IFN regulatory transcription factor (IRF) family. IRF-7 has been shown to play a role in the transcriptional activation of virus-inducible cellular proteins, including IFN beta chain proteins. Inducible expression of IRF-7 is largely restricted to lymphoid tissue. Four transcript variants encoding distinct isoforms (A,B,C and D) have been identified for this (PMID:9786932).The active IRF7A exists as a dimer form ~80 kDa(PMID:11073981). The MW 67-70 kDa has been reported in some papers (PMID:9786932; 22951831).Various posttranslational modifications of IRF7 including phosphorylation, ubiquitination, sumoylation and acetylation are identified (PMID:22951831).This antibody is a rabbit polyclonal antibody raised against the C-terminal 349 amino acid residues of human IRF7 D. The molecular weight of Nonphosphorylated IRF7 cofractionated with the 44-kDa marker, approximating its predicted size. . In contrast, phosphorylated IRF7, which migrate more slowly on SDS-PAGE. This phosphorylated IRF7 was consistent with a size of 80 to 90 kDa. (PMID: 11073981 )

Protocols

Product Specific Protocols
WB protocol for IRF7 antibody 22392-1-APDownload protocol
IHC protocol for IRF7 antibody 22392-1-APDownload protocol
IP protocol for IRF7 antibody 22392-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
Paralichthys olivaceusWB

Fish Shellfish Immunol

PMID: 30641186

Authors - Xiao-Lu Guan
humanWB

J Vet Sci

Genetic characterization of bovine viral diarrhea virus strains in Beijing region of China and innate immune responses of PBMCs in persistently infected dairy cattle.

Authors - Xiao Gang Weng
humanWB

Mol Med Rep

TET2 is involved in DNA hydroxymethylation, cell proliferation and inflammatory response in keratinocytes.

Authors - Xinxin Liu
humanWB

Cancer Med

A regulatory mutant on TRIM26 conferring the risk of nasopharyngeal carcinoma by inducing low immune response.

Authors - Xiao-Ming Lyu
humanWB

J Immunol

Inducible TAP1 Negatively Regulates the Antiviral Innate Immune Response by Targeting the TAK1 Complex.

Authors - Zhangchuan Xia