IRS1 Polyclonal antibody

IRS1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17509-1-AP

Synonyms

HIRS 1, IRS 1, IRS1, IRS-1



Tested Applications

Positive WB detected inA549 cells, A431 cells, HEK-293 cells, PC-3 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

17509-1-AP targets IRS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity blunt snout bream, human, mouse, pig, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IRS1 fusion protein Ag11714
Full Name insulin receptor substrate 1
Calculated molecular weight 1242 aa, 132 kDa
Observed molecular weight 160-185 kDa
GenBank accession numberBC053895
Gene symbol IRS1
Gene ID (NCBI) 3667
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ins receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in ins receptor (IR) and ins-like growth factor I receptor (IGF-IR) signaling. IRS1 is phosphorylated by ins receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression. Mutations in this gene are associated with type II diabetes and susceptibility to ins resistance. IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa.

Protocols

Product Specific Protocols
WB protocol for IRS1 antibody 17509-1-APDownload protocol
IHC protocol for IRS1 antibody 17509-1-APDownload protocol
IF protocol for IRS1 antibody 17509-1-APDownload protocol
IP protocol for IRS1 antibody 17509-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
blunt snout breamWB

Fish Physiol Biochem

Dietary leucine affects glucose metabolism and lipogenesis involved in TOR/PI3K/Akt signaling pathway for juvenile blunt snout bream Megalobrama amblycephala.

Authors - Hualiang Liang
humanWB

Toxicol Appl Pharmacol

Suppression of miR-143 contributes to overexpression of IL-6, HIF-1α and NF-κB p65 in Cr(VI)-induced human exposure and tumor growth.

Authors - Lin Wang
mouseWB

Sci Rep

Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy.

Authors - Mengyang Liu
pigWB

Int J Mol Sci

miR-146b Inhibits Glucose Consumption by Targeting IRS1 Gene in Porcine Primary Adipocytes.

Authors - Yan-Ling Zhu
humanWB

Toxicology

Retinol binding protein 4 mediates MEHP-induced glucometabolic abnormalities in HepG2 cells.

Authors - Fei Wang
mouseWB

Biomed Pharmacother

Therapeutic potential of NaoXinTong Capsule on the developed diabetic nephropathy in db/db mice.

Authors - Shu Yang