|Positive WB detected in||A549 cells, A431 cells, HEK-293 cells, PC-3 cells|
|Positive IP detected in||A549 cells|
|Positive IHC detected in||human breast cancer tissue, human liver tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A549 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
17509-1-AP targets IRS1 in WB, IP, IHC, IF, Dot blot,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, Megalobrama amblycephala, mouse, pig, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IRS1 fusion protein Ag11714|
|Full Name||insulin receptor substrate 1|
|Calculated molecular weight||1242 aa, 132 kDa|
|Observed molecular weight||160-185 kDa|
|GenBank accession number||BC053895|
|Gene ID (NCBI)||3667|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Ins receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in ins receptor (IR) and ins-like growth factor I receptor (IGF-IR) signaling. IRS1 is phosphorylated by ins receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression. Mutations in this gene are associated with type II diabetes and susceptibility to ins resistance. IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa.
|Product Specific Protocols|
|WB protocol for IRS1 antibody 17509-1-AP||Download protocol|
|IHC protocol for IRS1 antibody 17509-1-AP||Download protocol|
|IF protocol for IRS1 antibody 17509-1-AP||Download protocol|
|IP protocol for IRS1 antibody 17509-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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