Tested Applications
| Positive WB detected in | A549 cells, A431 cells, HEK-293 cells, Calu-1 cells, PC-3 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human colon tissue, human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17509-1-AP targets IRS1 in WB, IHC, IF/ICC, FC (Intra), IP, Dot blot, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, pig, megalobrama amblycephala |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag11714 Product name: Recombinant human IRS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 895-1243 aa of BC053895 Sequence: EYVNIEFGSDQSGYLSGPVAFHSSPSVRCPSQLQPAPREEETGTEEYMKMDLGPGRRAAWQESTGVEMGRLGPAPPGAASICRPTRAVPSSRGDYMTMQMSCPRQSYVDTSPAAPVSYADMRTGIAAEEVSLPRATMAAASSSSAASASPTGPQGAAELAAHSSLLGGPQGPGGMSAFTRVNLSPNRNQSAKVIRADPQGCRRRHSSETFSSTPSATRVGNTVPFGAGAAVGGGGGSSSSSEDVKRHSSASFENVWLRPGELGGAPKEPAKLCGAAGGLENGLNYIDLDLVKDFKQCPQECTPEPQPPPPPPPHQPLGSGESSSTRRSSEDLSAYASISFQKQPEDRQ Predict reactive species |
| Full Name | insulin receptor substrate 1 |
| Calculated Molecular Weight | 1242 aa, 132 kDa |
| Observed Molecular Weight | 160-185 kDa |
| GenBank Accession Number | BC053895 |
| Gene Symbol | IRS1 |
| Gene ID (NCBI) | 3667 |
| RRID | AB_10596914 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P35568 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ins receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in ins receptor (IR) and ins-like growth factor I receptor (IGF-IR) signaling (PMID: 10669726). IRS1 is phosphorylated by ins receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression (PMID: 11259577). Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance (PMID: 30319284). IRS1 has been linked to the development and progression of various cancers, including breast, prostate, and pancreatic cancers (PMID: 36768755). IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for IRS1 antibody 17509-1-AP | Download protocol |
| IF protocol for IRS1 antibody 17509-1-AP | Download protocol |
| IHC protocol for IRS1 antibody 17509-1-AP | Download protocol |
| IP protocol for IRS1 antibody 17509-1-AP | Download protocol |
| WB protocol for IRS1 antibody 17509-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-IRS1 polyclonal antibody (Cat# 17509-1-AP) has 113 citations published in journals including Cell Metabolism, Molecular Cell, Cell Discovery, Autophagy, EMBO Reports, FASEB Journal, Diabetes, and Science Reports. Research fields span insulin signaling, type 2 diabetes, obesity, NAFLD, cancer, neurodegeneration, autophagy, mitochondrial biology, and gut microbiota–metabolism interactions.
| Species | Application | Title |
|---|---|---|
Cell Metab Augmentation of scleral glycolysis promotes myopia through histone lactylation | ||
Autophagy Cardiac fibroblast-derived CCN1 aggravates diabetic cardiomyopathy through ITGAV-ITGB1/integrin αvβ1-mediated autophagy inhibition. | ||
Autophagy BNIP3L/Nix-induced mitochondrial fission, mitophagy, and impaired myocyte glucose uptake are abrogated by PRKA/PKA phosphorylation. | ||
Cell Discov Spatiotemporal regulation of insulin signaling by liquid-liquid phase separation. | ||
Cell Discov Transgenerational inheritance of diabetes susceptibility in male offspring with maternal androgen exposure | ||
Mol Cell KARATE: PKA-induced KRAS4B-RHOA-mTORC2 supercomplex phosphorylates AKT in insulin signaling and glucose homeostasis |
