IRS1 Polyclonal antibody

IRS1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (4)

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 17509-1-AP

Synonyms

HIRS 1, IRS 1, IRS1, IRS-1

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Validation Data Gallery

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  WB analysis of A549 using 17509-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 17509-1-AP (IRS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 17509-1-AP

  IP Result of anti-IRS1 (IP:17509-1-AP, 5ug; Detection:17509-1-AP 1:1000) with A549 cells lysate 3500ug.

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  IHC staining of human breast cancer using 17509-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human breast cancer using 17509-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 17509-1-AP (IRS1 antibody) at dilution of 1:100 (under 10x lens).

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  IF Staining of A549 using 17509-1-AP

  Immunofluorescent analysis of A549 cells using 17509-1-AP (IRS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, A431 cells, HEK-293 cells, PC-3 cells
• Positive IP detected in: A549 cells
• Positive IHC detected in: human breast cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 17 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

17509-1-AP targets IRS1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, Megalobrama amblycephala, mouse, pig, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: IRS1 fusion protein Ag11714
• Full Name: insulin receptor substrate 1
• Calculated molecular weight: 1242 aa, 132 kDa
• Observed molecular weight: 160-185 kDa
• GenBank accession number: BC053895
• Gene symbol: IRS1
• Gene ID (NCBI): 3667
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Ins receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in ins receptor (IR) and ins-like growth factor I receptor (IGF-IR) signaling. IRS1 is phosphorylated by ins receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression. Mutations in this gene are associated with type II diabetes and susceptibility to ins resistance. IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa.

Publications

Species	Application	Title
Megalobrama amblycephala	WB	Fish Physiol Biochem; Dietary leucine affects glucose metabolism and lipogenesis involved in TOR/PI3K/Akt signaling pathway for juvenile blunt snout bream Megalobrama amblycephala.; Authors - Hualiang Liang
human	WB	Toxicol Appl Pharmacol; Suppression of miR-143 contributes to overexpression of IL-6, HIF-1α and NF-κB p65 in Cr(VI)-induced human exposure and tumor growth.; Authors - Lin Wang
mouse	WB	Food Nutr Res; Pentadecanoic acid promotes basal and insulin-stimulated glucose uptake in C2C12 myotubes.; Authors - Wen-Cheng Fu
mouse	WB	Sci Rep; Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy.; Authors - Mengyang Liu
human	WB	BMC Cancer; miR-203 inhibits cell proliferation and ERK pathway in prostate cancer by targeting IRS-1.; Authors - Yang Meng ; KD Validated
human,mouse	WB	Eur J Pharmacol; Eurocristatine, a plant alkaloid from Eurotium cristatum, alleviates insulin resistance in db/db diabetic mice via activation of PI3K/AKT signaling pathway.; Authors - Hui Zhang