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IRS1 Antibody 6 Publications

Rabbit Polyclonal| Catalog number: 17509-1-AP

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Con: 45 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

IRS1 Antibody WB A549 cells 17509-1-AP

A549 cells were subjected to SDS PAGE followed by western blot with 17509-1-AP(IRS1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours

IRS1 Antibody IP A549 cells 17509-1-AP

IP Result of anti-IRS1 (IP:17509-1-AP, 5ug; Detection:17509-1-AP 1:1000) with A549 cells lysate 3500ug.

IRS1 Antibody IHC human breast cancer tissue 17509-1-AP

Immunohistochemical of paraffin-embedded human breast cancer using 17509-1-AP(IRS1 antibody) at dilution of 1:100 (under 40x lens)

IRS1 Antibody IHC human breast cancer tissue 17509-1-AP

Immunohistochemical of paraffin-embedded human breast cancer using 17509-1-AP(IRS1 antibody) at dilution of 1:100 (under 10x lens)

IRS1 Antibody IF A549 cells 17509-1-AP

Immunofluorescent analysis of A549 cells using 17509-1-AP(IRS1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Species specificity:
human, blunt snout bream, mouse, pig
Positive WB detected in:
A549 cells, A431 cells, HEK-293 cells, PC-3 cells
Positive IP detected in:
A549 cells
Positive IHC detected in:
human breast cancer tissue, human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
A549 cells
Recommended dilution:
WB : 1:500-1:2000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:20-1:200
IF : 1:20-1:200

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
IRS1 fusion protein Ag11714
Full name:
insulin receptor substrate 1
Calculated molecular weight:
1242aa,132 kDa
Observed molecular weight:
160-185 kDa
GenBank accession number:
BC053895
Gene ID (NCBI):
3667
Gene symbol
IRS1
Synonyms
HIRS 1, insulin receptor substrate 1, IRS 1, IRS1

Background

Insulin receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in insulin receptor (IR) and insulin-like growth factor I receptor (IGF-IR) signaling. IRS1 is phosphorylated by insulin receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression. Mutations in this gene are associated with type II diabetes and susceptibility to insulin resistance. IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa.


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