IRS1 Polyclonal antibody
IRS1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (4)
Applications
WB, IP, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 17509-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, A431 cells, HEK-293 cells, PC-3 cells |
Positive IP detected in | A549 cells |
Positive IHC detected in | human breast cancer tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:2000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 16 publications below |
IHC | See 1 publications below |
IF | See 1 publications below |
Product Information
17509-1-AP targets IRS1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | blunt snout bream, human, mouse, rat, swine |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | IRS1 fusion protein Ag11714 |
Full Name | insulin receptor substrate 1 |
Calculated molecular weight | 1242 aa, 132 kDa |
Observed molecular weight | 160-185 kDa |
GenBank accession number | BC053895 |
Gene symbol | IRS1 |
Gene ID (NCBI) | 3667 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Ins receptor substrate 1 (IRS1) was the first cloned and characterized member of the IRS family which are involved in ins receptor (IR) and ins-like growth factor I receptor (IGF-IR) signaling. IRS1 is phosphorylated by ins receptor tyrosine kinase and is involved in various cellular processes including DNA repair fidelity, transcriptional activity, and cell growth can support tumor development and progression. Mutations in this gene are associated with type II diabetes and susceptibility to ins resistance. IRS1 has a predicted molecular weight of 132 kDa, however, as a result of its extensive serine phosphorylation it separates on a SDS gel as a band of approximately 160-185 kDa.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for IRS1 antibody 17509-1-AP | Download protocol |
IHC protocol for IRS1 antibody 17509-1-AP | Download protocol |
IF protocol for IRS1 antibody 17509-1-AP | Download protocol |
IP protocol for IRS1 antibody 17509-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Fish Physiol Biochem Dietary leucine affects glucose metabolism and lipogenesis involved in TOR/PI3K/Akt signaling pathway for juvenile blunt snout bream Megalobrama amblycephala. | ||
Toxicol Appl Pharmacol Suppression of miR-143 contributes to overexpression of IL-6, HIF-1α and NF-κB p65 in Cr(VI)-induced human exposure and tumor growth. | ||
Sci Rep Administration of Danhong Injection to diabetic db/db mice inhibits the development of diabetic retinopathy and nephropathy. | ||
BMC Cancer miR-203 inhibits cell proliferation and ERK pathway in prostate cancer by targeting IRS-1.
| ||
Eur J Pharmacol Eurocristatine, a plant alkaloid from Eurotium cristatum, alleviates insulin resistance in db/db diabetic mice via activation of PI3K/AKT signaling pathway. | ||
Br J Nutr Nano chitosan-zinc complex improves the growth performance and antioxidant capacity of the small intestine in weaned piglets. |