ISCA1 Polyclonal antibody

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  WB analysis using 20324-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20324-1-AP (ISCA1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

ISCA1 Polyclonal Antibody for WB, ELISA

Cat No. 20324-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

RP11 507D14.2, ISA1, hIscA, HBLD2

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Tested Applications

• Positive WB detected in: HepG2 cells, mouse cerebellum tissue, mouse heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20324-1-AP targets ISCA1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ISCA1 fusion protein Ag14188
• Full Name: iron-sulfur cluster assembly 1 homolog (S. cerevisiae)
• Calculated Molecular Weight: 129 aa, 14 kDa
• Observed Molecular Weight: 15 kDa
• GenBank Accession Number: BC002675
• Gene Symbol: ISCA1
• Gene ID (NCBI): 81689
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BUE6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ISCA1, also named as HBLD2 and hIscA, belongs to the hesB/iscA family. It is involved in the assembly of mitochondrial iron-sulfur proteins. ISCA1 probably involved in the binding of an intermediate of Fe/S cluster assembly. ISCA1 plays an important role in both mitochondrial and cytosolic iron-sulfur cluster biogenesis, and a notable component of the latter is the interaction between ISCA1 and IOP1.