ISYNA1 Polyclonal antibody

ISYNA1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 14142-1-AP

Synonyms

hINO1, hIPS, Ino1, INOS, IPS, IPS 1, ISYNA1, MI 1 P synthase, MIP synthase



Tested Applications

Positive WB detected inmouse testis tissue, HepG2 cells
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

14142-1-AP targets ISYNA1 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ISYNA1 fusion protein Ag5272
Full Name inositol-3-phosphate synthase 1
Calculated molecular weight 61 kDa
Observed molecular weight 61 kDa
GenBank accession numberBC066902
Gene symbol ISYNA1
Gene ID (NCBI) 51477
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Myo-inositol 3-phosphate synthase (ISYNA1/IP synthase) is a rate-limiting enzyme that catalyzes the first step in the biosynthesis of all inositol containing compounds. It converts glucose 6-phosphate to Myo-inositol 3-phosphate.(PMID:21841945). The native enzyme is typically a homotrimermade up of 68-kDa subunits in mammalian cells and a homotetramer in yeast and plant cells. There are three new isoforms of 62, 43, and 16kDa detected suggesting ISYNA1 is a completely different holoenzyme. But only the brain and testis manifest the 68-kDa isoform, whereas the pancreas has a slightly smaller isoformat 67 kDa. The intestine has a completely unique isoformprofile comprising 62,43, 20kDa isoforms(PMID:19188364).

Protocols

Product Specific Protocols
WB protocol for ISYNA1 antibody 14142-1-APDownload protocol
IHC protocol for ISYNA1 antibody 14142-1-APDownload protocol
IF protocol for ISYNA1 antibody 14142-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Acta Pharmacol Sin

Ginsenoside Rb1 alleviates colitis in mice via activation of endoplasmic reticulum-resident E3 ubiquitin ligase Hrd1 signaling pathway.

Authors - Jian-Yi Dong
mouseWB

Mol Reprod Dev

Comparative proteomic analysis of proteins involved in oocyte meiotic maturation in mice.

Authors - Cao Senyang S
ratWB, IF

Alcohol Clin Exp Res

Ethanol supports macrophage recruitment and reinforces invasion and migration of lewis lung carcinoma.

Authors - Keke Yu