Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, SMMC-7721 cells, SW480 cells, HT-29 cells |
| Positive IHC detected in | human liver tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22146-1-AP targets Integrin alpha 1/CD49a in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17584 Product name: Recombinant human Integrin alpha-1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 792-1141 aa of BC137121 Sequence: DSLPNSVHEYIPFAKDCGNKEKCISDLSLHVATTEKDLLIVRSQNDKFNVSLTVKNTKDSAYNTRTIVHYSPNLVFSGIEAIQKDSCESNHNITCKVGYPFLRRGEMVTFKILFQFNTSYLMENVTIYLSATSDSEEPPETLSDNVVNISIPVKYEVGLQFYSSASEYHISIAANETVPEVINSTEDIGNEINIFYLIRKSGSFPMPELKLSISFPNMTSNGYPVLYPTGLSSSENANCRPHIFEDPFSINSGKKMTTSTDHLKRGTILDCNTCKFATITCNLTSSDISQVNVSLILWKPTFIKSYFSSLNLTIRGELRSENASLVLSSSNQKRELAIQISKDGLPGRVP Predict reactive species |
| Full Name | integrin, alpha 1 |
| Calculated Molecular Weight | 1179 aa, 131 kDa |
| Observed Molecular Weight | 180 kDa |
| GenBank Accession Number | BC137121 |
| Gene Symbol | Integrin alpha 1 |
| Gene ID (NCBI) | 3672 |
| RRID | AB_2879008 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P56199 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Integrin alpha 1/CD49a antibody 22146-1-AP | Download protocol |
| WB protocol for Integrin alpha 1/CD49a antibody 22146-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Integrin alpha 1/CD49a Polyclonal antibody (Cat# 22146-1-AP) has 18 citations published in journals including Nature Communications, ACS Nano, Science Advances, Cell Death & Disease, Cancer Letters, Cell Reports, International Journal of Biological Sciences, International Journal of Oncology, and others. Research fields span multiple cancer types (breast, liver, melanoma, colorectal, cervical, prostate, bladder, ovarian, glioma, pancreatic), nerve regeneration, cell adhesion, inflammation, fibrosis, and mechanobiology.
| Species | Application | Title |
|---|---|---|
Nat Commun Self-assembly of hybrid 3D cultures by integrating living and synthetic cells. | ||
ACS Nano Chiral Hydrogel Nerve Conduit Boosts Peripheral Nerve Regeneration via Regulation of Schwann Cell Reprogramming | ||
Sci Adv HSF2 drives breast cancer progression by acting as a stage-specific switch between proliferation and invasion. | ||
Cell Death Dis Exosome-derived ENO1 regulates integrin α6β4 expression and promotes hepatocellular carcinoma growth and metastasis. | ||
Cancer Lett Hepatocyte growth factor promotes melanoma metastasis through ubiquitin-specific peptidase 22-mediated integrins upregulation | ||
Int J Biol Sci Integrin α1 promotes tumorigenicity and progressive capacity of colorectal cancer.
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Reviews
What customers say
One reviewer (Boyan Zhang, 2019) rated this antibody 3/5 for Western Blot on MEF cells. They reported that the antibody labeled a band above 250 kD, likely the glycosylated form of Itga1, but did not detect a band around 170 kD as shown in the datasheet, suggesting a discrepancy between expected and observed molecular weights.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (04-24-2019) | This antibody labelled a band above 250 kD, which is likely the glycosylated form of Itga1. But, it did not label any band around 170 kD as shown in the data sheet.
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