|Positive WB detected in||HepG2 cells, SMMC-7721 cells, HeLa cells|
|Positive IHC detected in||human liver tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 22146-1-AP is Integrin alpha-1 Fusion Protein expressed in E. coli.
|Cited Reactivity||human, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Integrin alpha-1 fusion protein Ag17584|
|Full Name||integrin, alpha 1|
|Calculated molecular weight||1179 aa, 131 kDa|
|Observed molecular weight||180 kDa|
|GenBank accession number||BC137121|
|Gene ID (NCBI)||3672|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).
|Product Specific Protocols|
|WB protocol for Integrin alpha-1 antibody 22146-1-AP||Download protocol|
|IHC protocol for Integrin alpha-1 antibody 22146-1-AP||Download protocol|
|FC protocol for Integrin alpha-1 antibody 22146-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (04-24-2019)
This antibody labelled a band above 250 kD, which is likely the glycosylated form of Itga1. But, it did not label any band around 170 kD as shown in the data sheet.