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  • KD/KO Validated

Integrin alpha-1 Polyclonal antibody

Integrin alpha-1 Polyclonal Antibody for FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (1)





Cat no : 22146-1-AP


CD49a, Integrin alpha 1, integrin, alpha 1, ITGA1, VLA 1, VLA1

Tested Applications

Positive IHC detected inhuman liver tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells

Recommended dilution

Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 22146-1-AP is Integrin alpha-1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin alpha-1 fusion protein Ag17584
Full Name integrin, alpha 1
Calculated Molecular Weight 1179 aa, 131 kDa
Observed Molecular Weight 180 kDa
GenBank Accession NumberBC137121
Gene Symbol ITGA1
Gene ID (NCBI) 3672
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID:19118207). Integrin alpha-1 (ITGA1, CD49a) combines with the beta 1 subunit (ITGB1, CD29) to form a cell-surface receptor for collagen and laminin. This receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. Integrin alpha-1 is a transmembrane glycoprotein with an apparent molecular weight of 180-250 kDa, larger than the calculated molecular weight of 131 kDa (PMID: 2475259; 3257425; 11557277; 18840653).


Product Specific Protocols
WB protocol for Integrin alpha-1 antibody 22146-1-APDownload protocol
IHC protocol for Integrin alpha-1 antibody 22146-1-APDownload protocol
FC protocol for Integrin alpha-1 antibody 22146-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Int J Biol Sci

Integrin α1 promotes tumorigenicity and progressive capacity of colorectal cancer.

Authors - Hai Li
  • KD Validated

Int J Oncol

Downregulation of METTL6 mitigates cell progression, migration, invasion and adhesion in hepatocellular carcinoma by inhibiting cell adhesion molecules


J Ethnopharmacol

A combination index and glycoproteomics-based approach revealed synergistic anticancer effects of curcuminoids of turmeric against prostate cancer PC3 cells.

Authors - Meng-Meng Wei

Int J Oncol

FAM83A exerts tumor‑suppressive roles in cervical cancer by regulating integrins.

Authors - Junfen Xu

J Biomech

Integrin endocytosis on elastic substrates mediates mechanosensing.

Authors - Jing Li

Med Sci Monit

17β-Estradiol Protects Rat Annulus Fibrosus Cells Against Apoptosis via α1 Integrin-Mediated Adhesion to Type I Collagen: An In-vitro Study.

Authors - Chun-Ming Zhao


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Boyan (Verified Customer) (04-24-2019)

This antibody labelled a band above 250 kD, which is likely the glycosylated form of Itga1. But, it did not label any band around 170 kD as shown in the data sheet.

  • Applications: Western Blot,
  • Cell Tissue Type: MEF