|Positive WB detected in||Jurkat cells|
|Positive IHC detected in||human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
19676-1-AP targets Integrin Alpha 4 in WB, IHC, FC,ELISA applications and shows reactivity with human samples.
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)|
|Calculated molecular weight||115 kDa|
|Observed molecular weight||140-150 kDa|
|GenBank accession number||NM_000885|
|Gene ID (NCBI)||3676|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ITGA4, also named as CD49D, belongs to the integrin alpha chain family. ITGA4 is a receptor for fibronectin, VCAM1 and MADCAM1. ITGA4 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. It recognizes the sequence Q-I-D-S in VCAM1. I It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells ITGA4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells.
|Product Specific Protocols|
|WB protocol for Integrin Alpha 4 antibody 19676-1-AP||Download protocol|
|IHC protocol for Integrin Alpha 4 antibody 19676-1-AP||Download protocol|
|FC protocol for Integrin Alpha 4 antibody 19676-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (03-11-2019)
For WB, the expected band was very weak, and was much weaker than those non-specific bands; for IF, it could label the cell cortex localisation of Itga4, partially co-localised with Actin.