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Integrin Alpha-5 Polyclonal antibody

Integrin Alpha-5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, monkey and More (1)





Cat no : 10569-1-AP


CD49e, FNRA, Integrin alpha 5, Integrin alpha F, Integrin Alpha-5, ITGA5, VLA 5, VLA5A

Tested Applications

Positive WB detected inA549 cells, 3T3-L1 cells, COS-7 cells, HeLa cells, human placenta tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10569-1-AP targets Integrin Alpha-5 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, monkey samples.

Tested Reactivity human, mouse, monkey
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin Alpha-5 fusion protein Ag0860
Full Name integrin, alpha 5 (fibronectin receptor, alpha polypeptide)
Calculated Molecular Weight 115 kDa
Observed Molecular Weight 135-150 kDa
GenBank Accession NumberBC008786
Gene Symbol ITGA5
Gene ID (NCBI) 3678
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin alpha 5 (ITGA5), belongs to the integrin alpha chain family, primarily binds to Integrin beta 1 (ITGB1) to form an α5β1 heterodimer (PMID: 33711961). Integrin α5β1 is a specific receptor of fibronectin through its arginine-glycine-aspartic acid (RGD) binding site (PMID: 19608542). Integrin α5β1 plays roles in cell adhesion, migration and matrix formation, and has emerged as an essential mediator in many human carcinomas (PMID: 33408483).


Product Specific Protocols
WB protocol for Integrin Alpha-5 antibody 10569-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Boyan (Verified Customer) (02-04-2019)

Excellent. In WB, it gives a protein band at the expected molecular weight, and this band was decreased during adipocyte differentiation, which is consistent with those reported in literature. In IF, it labels endogenous protein at the cell surface, as that reported in literature.

  • Applications: Western Blot, Immunofluorescence,