CD11c/Integrin Alpha X Polyclonal antibody

CD11c/Integrin Alpha X Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, FC, Cell treatment, ELISA

Conjugate

Unconjugated

Cat no : 17342-1-AP

Synonyms

CD11C, Integrin alpha X, ITGAX, Leu M5, SLEB6



Tested Applications

Positive WB detected inhuman blood tissue
Positive IHC detected inhuman tonsillitis tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

17342-1-AP targets CD11c/Integrin Alpha X in WB, IHC, IF, FC, Cell treatment, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD11c/Integrin Alpha X fusion protein Ag11380
Full Name integrin, alpha X (complement component 3 receptor 4 subunit)
Calculated molecular weight 1169 aa, 129 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC038237
Gene symbol ITGAX
Gene ID (NCBI) 3687
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated α and β subunits. CD11c/Integrin alpha X is a 145-150 kDa type I transmembrane glycoprotein present on a variety of cells, including monocytes/macrophages, granulocytes, NK cells and dendritic cells. Integrin alpha X/beta 2 acts a receptor for fibrinogen and is important in monocyte adhesion and chemotaxis.

Protocols

Product Specific Protocols
WB protocol for CD11c/Integrin Alpha X antibody 17342-1-APDownload protocol
IHC protocol for CD11c/Integrin Alpha X antibody 17342-1-APDownload protocol
FC protocol for CD11c/Integrin Alpha X antibody 17342-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseFC

Biomaterials

Light-controlled oxygen production and collection for sustainable photodynamic therapy in tumor hypoxia.

Authors - Haoran Wang
humanIF

J Immunother Cancer

TSH-TSHR axis promotes tumor immune evasion.

Authors - Zhenghao Wu
mouseFC

Bioact Mater

Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.

Authors - Haoran Wang
mouseIF

Theranostics

Encapsulation of LXR ligand by D-Nap-GFFY hydrogel enhances anti-tumorigenic actions of LXR and removes LXR-induced lipogenesis.

Authors - Ke Feng
mouseIHC

Biomaterials

An injectable, low-toxicity phospholipid-based phase separation gel that induces strong and persistent immune responses in mice.

Authors - Lu Han
humanIHC

Cell Death Differ

DC-SIGN-LEF1/TCF1-miR-185 feedback loop promotes colorectal cancer invasion and metastasis.

Authors - Menglang Yuan