CD61 / Integrin Beta 3 Polyclonal antibody

CD61 / Integrin Beta 3 Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 18309-1-AP


CD61, GP3A, GPIIIa, Integrin beta 3, Integrin beta-3, ITGB3

Tested Applications

Positive WB detected inHuman peripheral blood platelets, mouse spleen tissue, rat spleen tissue
Positive IP detected inmouse spleen tissue

Recommended dilution

Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18309-1-AP targets CD61 / Integrin Beta 3 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, Hamster
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name integrin, beta 3 (platelet glycoprotein IIIa, antigen CD61)
Calculated Molecular Weight 87 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC127667
Gene Symbol ITGB3
Gene ID (NCBI) 3690
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrin beta-3, also named as CD61 and GPIIIa, is a receptor for cytotactin, fibronectin, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin, vitronectin and von Willebrand factor. Integrin beta-3 recognize the sequence R-G-D in a wide array of ligands and the sequence H-H-L-G-G-G-A-K-Q-A-G-D-V in fibrinogen gamma chain. Following activation integrin beta-3 brings about platelet/platelet interaction through binding of soluble fibrinogen which leads to rapid platelet aggregation which physically plugs ruptured endothelial surface. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. Human integrin beta-3 has a calculated molecular weight of 87 kDa. As a result of glycosylation, the apparent molecular mass of integrin beta-3 is approximately 90-110 kDa.


Product Specific Protocols
WB protocol for CD61 / Integrin Beta 3 antibody 18309-1-APDownload protocol
IP protocol for CD61 / Integrin Beta 3 antibody 18309-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Sci Adv

Platelet-derived porous nanomotor for thrombus therapy.

Authors - Mimi Wan

Nat Commun

Targeting Gα13-integrin interaction ameliorates systemic inflammation.

Authors - Ni Cheng


Construction and evaluation of novel αvβ3 integrin ligand-conjugated ultrasmall star polymer micelles targeted glomerular podocytes through GFB permeation.

Authors - Chengyuan Huang


A blood circulation-prolonging peptide anchored biomimetic phage-platelet hybrid nanoparticle system for prolonged blood circulation and optimized anti-bacterial performance.

Authors - Peipei Jin

Thromb Res

Repurposing of rilpivirine for preventing platelet β3 integrin-dependent thrombosis by targeting c-Src active autophosphorylation

Authors - Kui Liu

NPJ Regen Med

hUC-MSCs-derived MFGE8 ameliorates locomotor dysfunction via inhibition of ITGB3/ NF-κB signaling in an NMO mouse model

Authors - Huiming Xu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Sathyanarayani (Verified Customer) (04-05-2023)

I would highly recommend this primary antibody if you are looking for ITGB3. It produced really clear strong bands at 100kDa MW when used for western blot.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: mouse lysed blood

Sathyanarayani (Verified Customer) (04-05-2023)

The ITGB3 antibody produced really nice clear bands on the western blot at 110Kda MW. I would highly recommend using this if you are measuring ITGB3.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse blood