Tested Applications
| Positive WB detected in | BxPC-3 cells, A431 cells, A549 cells, HT-29 cells, HeLa cells, MCF-7 cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | mouse colon tissue, human placenta tissue, human pancreas cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21738-1-AP targets Integrin beta 4 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16450 Product name: Recombinant human Integrin beta-4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-354 aa of BC126411 Sequence: ANRCKKAPVKSCTECVRVDKDCAYCTDEMFRDRRCNTQAELLAAGCQRESIVVMESSFQITEETQIDTTLRRSQMSPQGLRVRLRPGEERHFELEVFEPLESPVDLYILMDFSNSMSDDLDNLKKMGQNLARVLSQLTSDYTIGFGKFVDKVSVPQTDMRPEKLKEPWPNSDPPFSFKNVISLTEDVDEFRNKLQGERISGNLDAPEGGFDAILQTAVCTRDIGWRPDSTHLLVFSTESAFHYEADGANVLAGIMSRNDERCHLDTTGTYTQYRTQDYPSVPTLVRLLAKHNIIPIFAVTNYSYSYYEKLHTYFPVSSLGVLQEDSSN Predict reactive species |
| Full Name | integrin, beta 4 |
| Calculated Molecular Weight | 1752 aa, 195 kDa |
| Observed Molecular Weight | 200-240 kDa |
| GenBank Accession Number | BC126411 |
| Gene Symbol | Integrin beta 4 |
| Gene ID (NCBI) | 3691 |
| RRID | AB_10733888 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P16144 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Integrin beta 4 antibody 21738-1-AP | Download protocol |
| IHC protocol for Integrin beta 4 antibody 21738-1-AP | Download protocol |
| IP protocol for Integrin beta 4 antibody 21738-1-AP | Download protocol |
| WB protocol for Integrin beta 4 antibody 21738-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-ITGB4 antibody (Cat# 21738-1-AP) has accumulated 39 citations across 37 journals, including Nature Communications, Cell Host & Microbe, Oncogene, FASEB Journal, Journal of Biological Chemistry, and International Journal of Molecular Sciences. Associated research fields primarily cover oncology—spanning lung, bladder, gastric, pancreatic, breast, cervical, and colorectal cancers—as well as epithelial-mesenchymal transition, metastasis, chemoresistance, immune evasion, liver fibrosis, airway disease, tissue regeneration, and antiviral research.
| Species | Application | Title |
|---|---|---|
Cell Host Microbe Peptostreptococcus stomatis promotes colonic tumorigenesis and receptor tyrosine kinase inhibitor resistance by activating ERBB2-MAPK
| ||
Nat Commun Eukaryote initiation factor 6 modulates small-cell lung carcinoma plasticity via the integrin-FAK signaling axis. | ||
J Exp Clin Cancer Res Reciprocal regulation of integrin β4 and KLF4 promotes gliomagenesis through maintaining cancer stem cell traits.
| ||
MedComm (2020) Nicotinamide N-Methyltransferase Epigenetically Activates Fibronectin 1 Through H3K9me3 Remodeling in Clear Cell Renal Cell Carcinoma. | ||
Cell Death Dis SOAT1 regulates cholesterol metabolism to induce EMT in hepatocellular carcinoma | ||
Cell Commun Signal NEDD4L mediates ITGB4 ubiquitination and degradation to suppress esophageal carcinoma progression |
Reviews
What customers say
The single review for 21738-1-AP is from Boyan Zhang (November 2024), who used the antibody for Western Blot with lot 00069062. He rated it 5 stars and noted that it recognized a protein at the expected size. Overall, the feedback is positive, confirming the antibody performs as expected in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (11-25-2024) | recognized a protein at the expected size
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