- Featured Product
- KD/KO Validated
Integrin Beta 4 Polyclonal antibody
Integrin Beta 4 Polyclonal Antibody for FC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, CoIP,ELISA
Conjugate
Unconjugated
Cat no : 21738-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | BxPC-3 cells, MCF-7 cells, A431 cells, A549 cells, HT-29 cells, HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human pancreas cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IHC | See 3 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
The immunogen of 21738-1-AP is Integrin Beta 4 Fusion Protein expressed in E. coli.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Integrin Beta 4 fusion protein Ag16450 |
| Full Name | integrin, beta 4 |
| Calculated molecular weight | 1752 aa, 195 kDa |
| Observed molecular weight | 200-240 kDa |
| GenBank accession number | BC126411 |
| Gene symbol | ITGB4 |
| Gene ID (NCBI) | 3691 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Integrin Beta 4 antibody 21738-1-AP | Download protocol |
| IHC protocol for Integrin Beta 4 antibody 21738-1-AP | Download protocol |
| IP protocol for Integrin Beta 4 antibody 21738-1-AP | Download protocol |
| FC protocol for Integrin Beta 4 antibody 21738-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res Reciprocal regulation of integrin β4 and KLF4 promotes gliomagenesis through maintaining cancer stem cell traits.
| ||
J Cell Biochem 17-allylamino-17-demethoxygeldanamycin impeded chemotherapy through antioxidant activation via reducing reactive oxygen species-induced cell death. | ||
Int J Oncol FAM83A exerts tumor‑suppressive roles in cervical cancer by regulating integrins. | ||
Sci Rep Integrin β4 promotes cell invasion and epithelial-mesenchymal transition through the modulation of Slug expression in hepatocellular carcinoma. | ||
Oncogene Programmed death ligand 1 promotes lymph node metastasis and glucose metabolism in cervical cancer by activating integrin β4/SNAI1/SIRT3 signaling pathway.
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