Integrin Beta 4 Polyclonal antibody

• Featured Product
• KD/KO Validated

Integrin Beta 4 Polyclonal Antibody for FC, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 21738-1-AP

Synonyms

CD104, GP150, Integrin beta 4, integrin, beta 4, ITGB4

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Validation Data Gallery

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  WB analysis using 21738-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21738-1-AP (Integrin beta-4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 21738-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 21738-1-AP (ITGB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 21738-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 21738-1-AP (ITGB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 21738-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21738-1-AP (ITGB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 21738-1-AP

  IP Result of anti-Integrin beta-4 (IP:21738-1-AP, 4ug; Detection:21738-1-AP 1:500) with HeLa cells lysate 2400ug.

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  IHC staining of human pancreas cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21738-1-AP (Integrin beta-4 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 21738-1-AP (Integrin beta-4 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human pancreas cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 21738-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 21738-1-AP (ITGB4 antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HeLa using 21738-1-AP

  1X10^6 HeLa cells were stained with 0.2ug Integrin beta-4 antibody (21738-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: BxPC-3 cells, MCF-7 cells, A431 cells, A549 cells, HT-29 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human pancreas cancer tissue, human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 4 publications below
• IF: See 2 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 21738-1-AP is Integrin Beta 4 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Integrin Beta 4 fusion protein Ag16450
• Full Name: integrin, beta 4
• Calculated molecular weight: 1752 aa, 195 kDa
• Observed molecular weight: 200-240 kDa
• GenBank accession number: BC126411
• Gene symbol: ITGB4
• Gene ID (NCBI): 3691
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.

Publications

Species	Application	Title
human	WB,IHC	J Exp Clin Cancer Res; Reciprocal regulation of integrin β4 and KLF4 promotes gliomagenesis through maintaining cancer stem cell traits.; Authors - Binbin Ma ; KD Validated
human	WB	J Cell Biochem; 17-allylamino-17-demethoxygeldanamycin impeded chemotherapy through antioxidant activation via reducing reactive oxygen species-induced cell death.; Authors - Yueqi Li
human	IF	Int J Oncol; FAM83A exerts tumor‑suppressive roles in cervical cancer by regulating integrins.; Authors - Junfen Xu
human	WB,IHC	Cell Commun Signal; METTL14-mediated N6-methyladenosine modification of ITGB4 mRNA inhibits metastasis of clear cell renal cell carcinoma.; Authors - Zhuonan Liu ; KD Validated
human	WB,IHC,IF	Sci Rep; Integrin β4 promotes cell invasion and epithelial-mesenchymal transition through the modulation of Slug expression in hepatocellular carcinoma.; Authors - Xiao-Long Li
human,mouse	WB,IHC,CoIP	Oncogene; Programmed death ligand 1 promotes lymph node metastasis and glucose metabolism in cervical cancer by activating integrin β4/SNAI1/SIRT3 signaling pathway.; Authors - Shaojia Wang ; KD Validated

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