- Featured Product
- KD/KO Validated
Integrin Beta 4 Polyclonal antibody
Integrin Beta 4 Polyclonal Antibody for FC, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 21738-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | BxPC-3 cells, MCF-7 cells, A431 cells, A549 cells, HT-29 cells, HeLa cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | human pancreas cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive FC detected in | HeLa cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 3 publications below |
WB | See 5 publications below |
IHC | See 4 publications below |
IF | See 2 publications below |
CoIP | See 1 publications below |
Product Information
The immunogen of 21738-1-AP is Integrin Beta 4 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Integrin Beta 4 fusion protein Ag16450 |
Full Name | integrin, beta 4 |
Calculated molecular weight | 1752 aa, 195 kDa |
Observed molecular weight | 200-240 kDa |
GenBank accession number | BC126411 |
Gene symbol | ITGB4 |
Gene ID (NCBI) | 3691 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Integrin Beta 4 antibody 21738-1-AP | Download protocol |
IHC protocol for Integrin Beta 4 antibody 21738-1-AP | Download protocol |
IP protocol for Integrin Beta 4 antibody 21738-1-AP | Download protocol |
FC protocol for Integrin Beta 4 antibody 21738-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Exp Clin Cancer Res Reciprocal regulation of integrin β4 and KLF4 promotes gliomagenesis through maintaining cancer stem cell traits.
| ||
J Cell Biochem 17-allylamino-17-demethoxygeldanamycin impeded chemotherapy through antioxidant activation via reducing reactive oxygen species-induced cell death. | ||
Int J Oncol FAM83A exerts tumor‑suppressive roles in cervical cancer by regulating integrins. | ||
Cell Commun Signal METTL14-mediated N6-methyladenosine modification of ITGB4 mRNA inhibits metastasis of clear cell renal cell carcinoma.
| ||
Sci Rep Integrin β4 promotes cell invasion and epithelial-mesenchymal transition through the modulation of Slug expression in hepatocellular carcinoma. | ||
Oncogene Programmed death ligand 1 promotes lymph node metastasis and glucose metabolism in cervical cancer by activating integrin β4/SNAI1/SIRT3 signaling pathway.
|