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Integrin Beta 4 Polyclonal antibody

Integrin Beta 4 Polyclonal Antibody for WB, IP, IF, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 21738-1-AP


CD104, GP150, Integrin beta 4, integrin, beta 4, ITGB4

Tested Applications

Positive WB detected inBxPC-3 cells, A431 cells, A549 cells, HT-29 cells, HeLa cells, MCF-7 cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse colon tissue, human placenta tissue, human pancreas cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Positive FC detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21738-1-AP targets Integrin Beta 4 in WB, IP, IF, FC, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Integrin Beta 4 fusion protein Ag16450
Full Name integrin, beta 4
Calculated Molecular Weight 1752 aa, 195 kDa
Observed Molecular Weight 200-240 kDa
GenBank Accession NumberBC126411
Gene Symbol ITGB4
Gene ID (NCBI) 3691
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-4 is predominantly expressed in squamous epithelial cells, endothelial cells, immature thymocytes, Schwann cells, and fibroblasts of the peripheral nervous system (PMID: 28084395). Integrin beta-4 pairs with alpha-6, and the heterodimeric integrin alpha-6/beta-4 is a receptor for laminin. Integrin alpha-6/beta-4 plays a critical structural role in the hemidesmosome of epithelial cells, and also plays a role in the invasive and metastatic phenotype of various cancers.


Product Specific Protocols
WB protocol for Integrin Beta 4 antibody 21738-1-APDownload protocol
IHC protocol for Integrin Beta 4 antibody 21738-1-APDownload protocol
IF protocol for Integrin Beta 4 antibody 21738-1-APDownload protocol
IP protocol for Integrin Beta 4 antibody 21738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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