ITPR3 Polyclonal antibody

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  WB analysis using 20729-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20729-1-AP (ITPR3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse small intestine using 20729-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20729-1-AP (ITPR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 20729-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20729-1-AP (ITPR3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

ITPR3 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 20729-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

InsP3R3, IP3 receptor isoform 3, IP3R, IP3R 3, IP3R3, ITPR3, Type 3 InsP3 receptor

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IHC detected in: mouse colon tissue, mouse small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

20729-1-AP targets ITPR3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: inositol 1,4,5-triphosphate receptor, type 3
• Calculated Molecular Weight: 304 kDa
• Observed Molecular Weight: 250-300 kDa
• GenBank Accession Number: NM_002224
• Gene Symbol: ITPR3
• Gene ID (NCBI): 3710
• RRID: AB_3669343
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q14573
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ITPRs family have three members: ITPR1, ITPR2 and ITPR3. For ITPR3, it is associated with cervical squamous cell carcinoma, glioblastoma, cholangiocarcinoma and other cancers. Inositol 1,4, 5-triphosphate receptor type 3 (ITPR3) was found to be apical-located in bile duct cells, and disruption of lipid raft in the segregated bile duct unit (IBDU) redistributes ITPR3 and impairs Ca2+ waves. (PMID: 35580861, PMID:35852334)

Publications

Species	Application	Title
mouse	WB	J Ethnopharmacol; Zhizichi decoction alleviates depressive-like behaviors through modulating mitochondria-associated membrane via the IP3R3-GRP75-VDAC1 complex; Authors - Ye Liu
human	WB	Mol Cell Endocrinol; High glucose elevates intracellular calcium level and induces ferroptosis in glomerular endothelial cells through the miR-223-3p/ITPR3 pathway; Authors - Dekai Wang