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IkBa antibody (mAb) (Clone 6A920)

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB

CloneNo.

6A920

Activ Motif logo

Cat No : 40903 40903

Synonyms

IKBA, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor, Alpha, NFKBI, ikB-alpha, IKBA, NF-Kappa-B Inhibitor Alpha, Major Histocompatibility Complex Enhancer-Binding Protein MAD3, Nuclear Factor Of Kappa Light Chain Gene Enhancer In B-Cells, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, MAD3, antibody, antibodies, monoclonal, mab, wb, western blotting, ip, immunoprecipitation

Validation Data Gallery

  • IkBa mAb tested by Western blot. The anti-IkBa detects both phosphorylated and non-phosphorylated forms of IkBa by Western blot. Jurkat cells (~1 x 107) were treated for indicated time periods with 10 nm/ml PMA, 1 uM ionomycin and anti-CD28 (1:10,000 dilution). 10 ul of cytoplasmic (C) and nuclear extract (N) were resolved on a 8.75% SDS-PAGE and transferred to Immobilon membrane. Figure is courtesy of Dr. Shao-Cong Sun at Penn State Univ. College of Medicine.

    IkBa mAb tested by Western blot. The anti-IkBa detects both phosphorylated and non-phosphorylated forms of IkBa by Western blot. Jurkat cells (~1 x 107) were treated for indicated time periods with 10 nm/ml PMA, 1 uM ionomycin and anti-CD28 (1:10,000 dilution). 10 ul of cytoplasmic (C) and nuclear extract (N) were resolved on a 8.75% SDS-PAGE and transferred to Immobilon membrane. Figure is courtesy of Dr. Shao-Cong Sun at Penn State Univ. College of Medicine.

Product Information

Tested Applications WB

Applications Validated by Active Motif: WB: 1 - 2 ug/ml dilution For optimal results, primary antibody incubations should be performed at room temperature. The addition of 0.1% Tween 20 to all blocking solutions may also reduce background. Individual optimization may be required.
Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen This IkBa antibody was raised against a recombinant protein corresponding to amino acid residues 32-291 of human IkBa.
Full Name IkBa antibody (mAb) (Clone 6A920)
Synonyms IKBA, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor, Alpha, NFKBI, ikB-alpha, IKBA, NF-Kappa-B Inhibitor Alpha, Major Histocompatibility Complex Enhancer-Binding Protein MAD3, Nuclear Factor Of Kappa Light Chain Gene Enhancer In B-Cells, I-kappa-B-alpha, IkB-alpha, IkappaBalpha, MAD3, antibody, antibodies, monoclonal, mab, wb, western blotting, ip, immunoprecipitation
Molecular weight 40 kDa
GenBank accession numberNP_065390
RRIDAB_2753150
Purification Method Affinity Purified
Buffer PBS containing 0.02% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

IkBa – NFkB (NFkB p50 & NFkB p65) signaling is controlled to a large extent by the sequestration of the NFkB complex in the cytoplasm by its association with one of the IkB family of proteins. IkBa is phosphorylated at Ser32 and Ser36 (IkBa phospho Ser32,36) by the IkB Kinase (IKK) complex, resulting in the degradation of IkB and the nuclear translocation of NFkB.