|Positive IHC detected in||human liver cancer tissue, human tonsillitis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 26156-1-AP is IL-2 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||IL-2 fusion protein Ag24079|
|Full Name||interleukin 2|
|Calculated molecular weight||19 kDa|
|GenBank accession number||NM-008366|
|Gene ID (NCBI)||16183|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
What is the calculated molecular weight of IL2?
Where is IL-2 produced?
IL-2 is primarily produced and secreted by CD4+ helper T cells in secondary lymphoid organs. CD8+ T cells, some B cells, and dendritic cells also produce IL-2.
What allows the protein to bind to its receptor?
The N-terminal 20 amino acids are essential for protein-receptor interaction.
What are the post-translational modifications of IL-2?
IL-2 may present glycosylation, yet the function of this modification has not been confirmed.
What is the role of IL-2?
IL-2 is a key immunoregulatory cytokine that promotes proliferation of helper T cells, upregulating its own secretion, while also increasing activity of regulatory T cells and providing negative feedback to its production and the immune response as a whole. IL-2 is also a growth factor for natural killer cells and an upregulator of B cell antibody expression and secretion.
What activates the IL-2 pathway?
Combined T cell antigen receptor and CD28 signals allow for maximum activation of the IL-2 pathway.
How does IL-2 expression change during an immune response?
During an immune response, CD4+ and CD8+ T cells will secrete increased amounts of IL-2 to be consumed by regulatory T cells.
How do the responses to strong and weak IL-2 signals differ?
Strong IL-2 levels promote antigen-specific T cells to differentiate into effector T cells, responding immediately to a stimulus or threat. Weak IL-2 signals, however, result in effector T cells to differentiate into helper or memory T cells.
What negatively regulates IL-2?
Immunoregulatory CD4+CD5+ T cells in inhibit IL-2 production by helper T cells.
(PMIDs: 22343569, 24376444, 15473953)
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