IL-2 Antibody

IL-2 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 26156-1-AP

Synonyms

Il 2, Il2, Il-2, interleukin 2



Tested Applications

Positive IHC detected inhuman liver cancer tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The 26156-1-AP immunogen is Fusion Protein IL-2 protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IL-2 fusion protein Ag24079
Full Name interleukin 2
Calculated molecular weight 19 kDa
Observed molecular weight
GenBank accession number NM-008366
Gene symbol Il2
Gene ID (NCBI) 16183
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

What is the calculated molecular weight of IL2?

19 kDa

Where is IL-2 produced?

IL-2 is primarily produced and secreted by CD4+ helper T cells in secondary lymphoid organs. CD8+ T cells, some B cells, and dendritic cells also produce IL-2.

What allows the protein to bind to its receptor?

The N-terminal 20 amino acids are essential for protein-receptor interaction.

What are the post-translational modifications of IL-2?

IL-2 may present glycosylation, yet the function of this modification has not been confirmed. 

What is the role of IL-2?

IL-2 is a key immunoregulatory cytokine that promotes proliferation of helper T cells, upregulating its own secretion, while also increasing activity of regulatory T cells and providing negative feedback to its production and the immune response as a whole. IL-2 is also a growth factor for natural killer cells and an upregulator of B cell antibody expression and secretion. 

What activates the IL-2 pathway?

Combined T cell antigen receptor and CD28 signals allow for maximum activation of the IL-2 pathway. 

How does IL-2 expression change during an immune response?

During an immune response, CD4+ and CD8+ T cells will secrete increased amounts of IL-2 to be consumed by regulatory T cells. 

How do the responses to strong and weak IL-2 signals differ?

Strong IL-2 levels promote antigen-specific T cells to differentiate into effector T cells, responding immediately to a stimulus or threat. Weak IL-2 signals, however, result in effector T cells to differentiate into helper or memory T cells. 

What negatively regulates IL-2?

Immunoregulatory CD4+CD5+ T cells in inhibit IL-2 production by helper T cells.

 

(PMIDs: 22343569, 24376444, 15473953)

Protocols

Product Specific Protocols
IHC protocol for IL-2 antibody 26156-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Lipids Health Dis

Arachidonic acid induces macrophage cell cycle arrest through the JNK signaling pathway.

Authors - Ziying Shen
humanIHC

J Cancer

IL-2 enhanced MHC class I expression in papillary thyroid cancer with Hashimoto's thyroiditis overcomes immune escape in vitro.

Authors - Jia-Qian Hu