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JAK1 Monoclonal antibody

JAK1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b


human, rat, mouse







Cat no : 66466-1-Ig


JAK 1, JAK1, JAK1A, JAK1B, Janus kinase 1, JTK3, Tyrosine protein kinase JAK1

Tested Applications

Positive WB detected inK-562 cells, Jurkat cells, rat spleen tissue, PC-12 cells, NIH/3T3 cells, RAW 264.7 cells, Ramos cells, Daudi cells
Positive IHC detected inhuman breast cancer tissue, human cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66466-1-Ig targets JAK1 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Reactivity human, rat, mouse
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen JAK1 fusion protein Ag17940
Full Name Janus kinase 1 (a protein tyrosine kinase)
Calculated Molecular Weight 1154 aa, 133 kDa
Observed Molecular Weight 133 kDa
GenBank Accession NumberBC132729
Gene Symbol JAK1
Gene ID (NCBI) 3716
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Janus kinase 1(JAK1), is a member of a class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. JAK1 is essential for signaling for certain type I and type II cytokines. JAK1 plays a critical role in initiating responses to multiple major cytokine receptor families. Expression of JAK1 in cancer cells enables individual cells to contract, potentially allowing them to escape their tumor and metastasize to other parts of the body.


Product Specific Protocols
WB protocol for JAK1 antibody 66466-1-IgDownload protocol
IHC protocol for JAK1 antibody 66466-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Adv Sci (Weinh)

EHBP1L1 Drives Immune Evasion in Renal Cell Carcinoma through Binding and Stabilizing JAK1

Authors - Yihui Pan

Cell Rep

Macrophage autophagy deficiency-induced CEBPB accumulation alleviates atopic dermatitis via impairing M2 polarization

Authors - Yongcheng Zhu

Cell Chem Biol

Small-molecule MHC-II inducers promote immune detection and anti-cancer immunity via editing cancer metabolism

Authors - Ling Huang

Antioxidants (Basel)

Fibroblast Upregulation of Vitamin D Receptor Represents a Self-Protective Response to Limit Fibroblast Proliferation and Activation during Pulmonary Fibrosis

Authors - Juan Wei

Antioxidants (Basel)

Periplaneta americana Extract Pretreatment Alleviates Oxidative Stress and Inflammation and Increases the Abundance of Gut Akkermansia muciniphila in Diquat-Induced Mice

Authors - Shiyi Lu

Oxid Med Cell Longev

Protective Effect of Methane-Rich Saline on Acetic Acid-Induced Ulcerative Colitis via Blocking the TLR4/NF-κB/MAPK Pathway and Promoting IL-10/JAK1/STAT3-Mediated Anti-inflammatory Response.

Authors - Guanghui Wang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Christine (Verified Customer) (07-12-2022)

Signal is at the expected molecular weight, very low aspecific staining on the membrane, signal is proportional to the lysate quantity loaded. Need quite a large quantity of lysates.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: RPE1 cells and Huh7 cells (human)
JAK1 Antibody Western Blot validation (1:1000 dilution) in RPE1 cells and Huh7 cells (human) (Cat no:66466-1-Ig)