JMJD2B / KDM4B antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ICC, IF, IP, WB

Cat No : 61221,61222

Synonyms

JMJD2B, KDM4B, JmjC domain-containing histone demethylation protein 3B Jumonji domain-containing protein 2B, JHDM3B, histone demethylase, H3K9me3, H3k9me, MLL2, ERα, transcriptional activation, stem cell, stem cells, sample

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Validation Data Gallery

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  JMJD2B / KDM4B antibody (pAb) tested by Immunofluorescence. Formaldehyde fixed HeLa cells stained with JMJD2B / KDM4B antibody at a 1:2,000 dilution.

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  JMJD2B / KDM4B antibody (pAb) tested by Immunoprecipitation. 4 ul of JMJD2B / KDM4B antibody was used to immunoprecipitate JMJD2B / KDM4B from 500 ug of K562 whole cell extract (lane 2). 4 ug of mouse IgG was used as a negative control (lane 1). The immunoprecipitated protein was detected by Western blotting using the JMJD2B / KDM4B antibody at a dilution of 1:500.

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  JMJD2B / KDM4B antibody (pAb) tested by Western blot. Nuclear extract of K-562 cells (40 ug) probed with JMJD2B antibody (pAb) at a dilution of 1:500.

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Product Information

• Tested Applications: ICC, IF, IP, WB

  Applications Validated by Active Motif: ICC/IF: 1:2,000 dilution IP: 10 ul per IP WB*: 1:500 - 1:1,000 dilution *Note: many chromatin-bound proteins are not soluble in a low salt nuclear extract and fractionate to the pellet. Therefore, we recommend a High Salt / Sonication Protocol when preparing nuclear extracts for Western blot. For JMJD2B / KDM4B, we also offer AbFlex JMJD2B / KDM4B Recombinant Antibody (rAb). For details, see Catalog No. 91305.

• Tested Reactivity: Human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: This JMJD2B / KDM4B antibody was raised against a peptide within the C-terminal region of human JMJD2B.
• Full Name: JMJD2B / KDM4B antibody (pAb)
• Synonyms: JMJD2B, KDM4B, JmjC domain-containing histone demethylation protein 3B Jumonji domain-containing protein 2B, JHDM3B, histone demethylase, H3K9me3, H3k9me, MLL2, ERα, transcriptional activation, stem cell, stem cells, sample
• Molecular weight: 142 kDa
• GenBank accession number: NP_055830
• RRID: AB_2615033
• Purification Method: Affinity Purified
• Buffer: Purified IgG in PBS with 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
• Storage: Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

Background Information

JMJD2B / KDM4B (JmjC domain-containing histone demethylation protein 3B, Jumonji domain-containing protein 2B, JHDM3B) is a histone demethylase that has been shown to demethylate histone H3 trimethylated at lysine 9. JMJD2B / KDM4B is physically associated with the MLL2 methyltransferase complex where it serves as a co-factor for ERa-mediated transcriptional activation.