JNK Polyclonal antibody

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• JNK Polyclonal antibody (51151-1-AP)

JNK Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 51151-1-AP Copied!

Publications (141)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

c Jun N terminal kinase 1, JNK 46, JNK1, JNK1A2, JNK21B1/2, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1

• 

  WB analysis using 51151-1-AP

  4T1 cells were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 51151-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 51151-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 51151-1-AP (JNK antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 51151-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon cancer using 51151-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 51151-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 51151-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 51151-1-AP (JNK antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of SH-SY5Y using 51151-1-AP

  Immunofluorescent analysis of (-20℃ Acetone) fixed SH-SY5Y cells using 51151-1-AP (JNK antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: 4T1 cells, mouse brain tissue, A431 cells, HEK-293 cells, HepG2 cells, Jurkat cells, Neuro-2a cells, C6 cells, NIH/3T3 cells
• Positive IHC detected in: human colon cancer tissue, human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

51151-1-AP targets JNK in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat, pig, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mitogen-activated protein kinase 8
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 44-48 kDa, 50-55 kDa
• GenBank Accession Number: NM_138982
• Gene Symbol: JNK
• Gene ID (NCBI): 5599
• RRID: AB_2140710
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P45983
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Function

JNK1, also known as mitogen-activated protein kinase 8 - MAPK8, is a serine/threonine-protein kinase. It is an important player in signaling transduction pathways governing cellular response to external stimuli, including ultraviolet radiation (UV), inflammatory cytokines, and reactive oxygen species (ROS). JNK1 phosphorylates several transcription factors, such as c-Jun, Elk-1, and ATF2, affecting their transcription activity.

Tissue specificity

JNK1 is ubiquitously expressed.

Involvement in disease

• JNK-mediated phosphorylation governs a variety of important cellular processes, and abnormalities of JNK1 activity have been implicated in obesity and cancer.

Isoforms

There are four isoforms of JNK1 (PMID: 8654373), giving rise to 46 kDa and 55 kDa isoforms. JNK1 isoforms differ in their substrate specificity and tissue expression profiles.

Post-translational modifications

JNK1 is phosphorylated by MAPK kinases (MAPKK).

Cellular localization

JNK1 is present both in the nucleus and in the cytoplasm. Stress conditions and other stimuli can cause a shift in JNK1 subcellular distribution.  

Publications

What published studies show

The JNK Polyclonal Antibody (Cat# 51151-1-AP) has 141 total citations published in journals including Nature Communications, Cell Research, Cell Death & Differentiation, Journal of Ethnopharmacology, Free Radical Biology and Medicine, FASEB Journal, and many others. Research fields covered include MAPK/JNK signaling, inflammation, apoptosis, cancer biology, cardiovascular disease, neuroprotection, liver injury, bone metabolism, pharmacology, and infectious diseases.

Automatically generated from the 141 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell Res; Mlkl knockout mice demonstrate the indispensable role of Mlkl in necroptosis.; Authors - Wu Jianfeng J
mouse	WB	Nat Commun; Pla2g7 regulates bone homeostasis via Alox12/12-HETE/Gpr31 signaling axis.; Authors - Jiayan Jin
human	WB,IP,CoIP	Nat Commun; Proteogenomics of different urothelial bladder cancer stages reveals distinct molecular features for papillary cancer and carcinoma in situ; Authors - Zhenmei Yao
rat	WB	Int J Oral Sci; Activation of mitogen-activated protein kinases in satellite glial cells of the trigeminal ganglion contributes to substance P-mediated inflammatory pain.; Authors - Yanyan Zhang
mouse, rat	WB	J Nanobiotechnology; Gut microbiota dysfunction mediates stress-exacerbated aortic dissection via the bacteroides vulgatus-outer membrane vesicles-stearic acid-JNK/MAPK axis.; Authors - Jiancheng Cheng
human	WB	J Exp Clin Cancer Res; BDH2 triggers ROS-induced cell death and autophagy by promoting Nrf2 ubiquitination in gastric cancer.; Authors - Jia-Zhou Liu