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KAT5 Polyclonal antibody

KAT5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10827-1-AP

Synonyms

60 kDa Tat interactive protein, cPLA(2) interacting protein, cPLA2, ESA1, Histone acetyltransferase KAT5, HIV 1 Tat interactive protein, HTATIP, HTATIP1, K(lysine) acetyltransferase 5, KAT5, Lysine acetyltransferase 5, PLIP, TIP, TIP60



Tested Applications

Positive WB detected inPC-3 cells, rat liver tissue, HepG2 cells, Jurkat cells, HeLa cells, mouse liver tissue
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10827-1-AP targets KAT5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KAT5 fusion protein Ag1261
Full Name K(lysine) acetyltransferase 5
Calculated molecular weight 60 kDa
Observed molecular weight 55-60 kDa
GenBank accession numberBC000166
Gene symbol KAT5
Gene ID (NCBI) 10524
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KAT5, also named as The histone acetyltransferase (HAT) Tat interactive protein 60 kD belongs to the MYST protein family that contains atypical zinc finger and histone acetyltransferase domains. Also, KAT5 contains a chromodomain. KAT5 is a catalytic subunit of the NuA4 histone acetyltransferase complex. The NuA4 HAT complex plays a role in transcriptional activation of select genes mainly by acetylation of nucleosomal histone H4 and H2A, which influence nucleosome-DNA interaction and promotes interaction of the modified histones with other proteins that could regulate transcription positively. This complex also involves in the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, DNA repair and apoptosis. KAT5 exists some isoforms with MV 53-60 kDa

Protocols

Product Specific Protocols
WB protocol for KAT5 antibody 10827-1-APDownload protocol
IHC protocol for KAT5 antibody 10827-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Biochim Biophys Acta

MDR1 mediated chemoresistance: BMI1 and TIP60 in action.

Authors - Soumyajit Banerjee Mustafi
ratIHC

Front Pediatr

Impaired FGF10 Signaling and Epithelial Development in Experimental Lung Hypoplasia With Esophageal Atresia.

Authors - Jun Wang
humanWB

Cell Physiol Biochem

Tip60 Suppresses Cholangiocarcinoma Proliferation and Metastasis via PI3k-AKT.

Authors - Yaodong Zhang
human

Hum Reprod

Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility.

Authors - Yi-Min Cheng
humanWB

Mol Cell Proteomics

Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).

Authors - Heguo Yu
humanWB

Nat Commun

Requirement for p62 acetylation in the aggregation of ubiquitylated proteins under nutrient stress.

Authors - Zhiyuan You
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