Kir2.1 Polyclonal antibody

Kir2.1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 19965-1-AP

Synonyms

HHBIRK1, HHIRK1, hIRK1, IRK 1, IRK1, KCNJ2, Kir2.1, LQT7, SQT3

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Validation Data Gallery

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  WB analysis of A549 using 19965-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19965-1-AP (Kir2.1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 19965-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 19965-1-AP (Kir2.1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 19965-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19965-1-AP (Kir2.1 antibody at dilution of 1:50.

• 

  IHC staining of human brain using 19965-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 19965-1-AP (Kir2.1 antibody at dilution of 1:50.

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Tested Applications

• Positive WB detected in: A549 cells
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 8 publications below
• IHC: See 2 publications below

Product Information

19965-1-AP targets Kir2.1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, rabbit
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: potassium inwardly-rectifying channel, subfamily J, member 2
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 50 kDa, 60 kDa
• GenBank Accession Number: NM_000891
• Gene Symbol: Kir2.1
• Gene ID (NCBI): 3759
• RRID: AB_10859827
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KCNJ2, also named as HHBIRK1, HHIRK1, IRK1, KIR2.1, LQT7 and SQT3, belongs to the inward rectifier-type potassium channel family. KCNJ2 probably participates in establishing action potential waveform and excitability of neuronal and muscle tissues. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. KCNJ2 can be blocked by extracellular barium or cesium. Defects in KCNJ2 are the cause of long QT syndrome type 7 (LQT7). Defects in KCNJ2 are the cause of short QT syndrome type 3 (SQT3). The antibody recognizes the C-term of KCNJ2.

Publications

Species	Application	Title
mouse	WB	Nat Commun; Kir2.1-mediated membrane potential promotes nutrient acquisition and inflammation through regulation of nutrient transporters.; Authors - Weiwei Yu
human	WB,IHC	Cell Commun Signal; KCNJ2/HIF1α positive-feedback loop promotes the metastasis of osteosarcoma; Authors - Mao Shen
human	WB	Stem Cell Res Ther; Overexpression of KCNJ2 enhances maturation of human-induced pluripotent stem cell-derived cardiomyocytes; Authors - Jingjun Zhou
rabbit	WB	J Mol Cell Cardiol; Altered long non-coding RNA expression profile in rabbit atria with atrial fibrillation: TCONS_00075467 modulates atrial electrical remodeling by sponging miR-328 to regulate CACNA1C.; Authors - Zhan Li
rabbit	WB	J Cell Mol Med; Long noncoding RNA TCONS-00106987 promotes atrial electrical remodelling during atrial fibrillation by sponging miR-26 to regulate KCNJ2.; Authors - Juanjuan Du
rat	WB	J Cell Mol Med; Nucleoporin 107 facilitates the nuclear export of Scn5a mRNA to regulate cardiac bioelectricity.; Authors - Yi Guan