VWA8 Polyclonal antibody

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  WB analysis using 31348-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 31348-1-AP (KIAA0564 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 31348-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31348-1-AP (VWA8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 31348-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 31348-1-AP (VWA8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 31348-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using VWA8 antibody (31348-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

VWA8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 31348-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RP97, P7BP2, KIAA0564

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Tested Applications

• Positive WB detected in: HepG2 cells
• Positive IHC detected in: mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31348-1-AP targets VWA8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: VWA8 fusion protein Ag35404
• Full Name: KIAA0564
• Calculated Molecular Weight: 215kDa，1905aa
• Observed Molecular Weight: 204-215 kDa, 117 kDa
• GenBank Accession Number: NM_015058.1
• Gene Symbol: KIAA0564
• Gene ID (NCBI): 23078
• RRID: AB_3669955
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: A3KMH1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

VWA8, also known as KIAA0564, is a mitochondrial matrix-targeted protein having a putative ATPase activity (PMID: 34660594). It has 3 isoforms with the MW of 215, 204, and 117 kDa.