• Featured Product
  • KD/KO Validated

TALPID3 Polyclonal antibody

TALPID3 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine and More (1)

Applications

WB, IF, IHC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 24421-1-AP

Synonyms

KIAA0586, KIAA0586 isoform, Talpid3



Tested Applications

Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24421-1-AP targets TALPID3 in WB, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, canine samples.

Tested Reactivity human, mouse, canine
Cited Reactivityhuman, mouse, Caenorhabditis elegans
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TALPID3 fusion protein Ag19813
Full Name KIAA0586
Calculated Molecular Weight 1533 aa, 169 kDa
GenBank Accession NumberBC066647
Gene Symbol TALPID3
Gene ID (NCBI) 9786
RRIDAB_2879539
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KIAA0586 also named as Talpid3 is a 1472 amino-acid protein, which belongs to Talpid3 family. Talpid3 is a gene essential for vertebrate development. Studies in chicken, mouse, zebrafish and human have shown that TALPID3 is a centrosomal protein, mutants null for TALPID3 fail to produce cilia, resulting in far reaching signalling defects such as polydactyly, dorsalised neural tube, craniofacial and vascular defects often due to abnormalities in Hedgehog signal transduction. Talpid3 is absolutely required for the function of both Gli repressor and activator in the intracellular Hedgehog pathway and ciliogenesis. TALPID3 may be identified as a subcellular maker of centrosome and needed to further studied.

Protocols

Product Specific Protocols
IHC protocol for TALPID3 antibody 24421-1-APDownload protocol
IF protocol for TALPID3 antibody 24421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,Caenorhabditis elegansWB,IF,CoIP

Nat Commun

TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating.

Authors - Hao Yan
  • KD Validated
mouseIF

Elife

TALPID3 controls centrosome and cell polarity and the human ortholog KIAA0586 is mutated in Joubert syndrome (JBTS23).

Authors - Louise A Stephen
humanIF

J Cell Biol

A ciliopathy complex builds distal appendages to initiate ciliogenesis.

Authors - Dhivya Kumar
humanIF

Elife

Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis

Authors - Emma A Hall
humanWB

EMBO Rep

Aurora Kinase A proximity map reveals centriolar satellites as regulators of its ciliary function.

Authors - Melis D Arslanhan
mouse

Development

Centrosomal protein CP110 controls maturation of mother centriole during cilia biogenesis.

Authors - Sharda Prasad Yadav

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Elisa (Verified Customer) (12-14-2022)

RPE1 cells were fixed in cold methanol for 10' at -20C. Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-488-Anti-rabbit was used as secondary antibody for Talpid3 (1:600 dilution) (1h at room temperature). In pink (Talpid3); in green (GT335, decorating centrioles) and in blue (Hoechst, DNA).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: RPE1 cells
TALPID3 Antibody Immunofluorescence validation (1:200 dilution) in RPE1 cells (Cat no:24421-1-AP)