TALPID3 Polyclonal antibody
TALPID3 Polyclonal Antibody for IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IHC, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 24421-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive IHC detected in | human brain tissue, Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 2 publications below |
WB | See 2 publications below |
IF | See 4 publications below |
CoIP | See 2 publications below |
Product Information
The immunogen of 24421-1-AP is TALPID3 Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse |
Cited Reactivity | C. elegans, human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TALPID3 fusion protein Ag19813 |
Full Name | KIAA0586 |
Calculated molecular weight | 1533 aa, 169 kDa |
GenBank accession number | BC066647 |
Gene symbol | KIAA0586 |
Gene ID (NCBI) | 9786 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KIAA0586 also named as Talpid3 is a 1472 amino-acid protein, which belongs to Talpid3 family. Talpid3 is a gene essential for vertebrate development. Studies in chicken, mouse, zebrafish and human have shown that TALPID3 is a centrosomal protein, mutants null for TALPID3 fail to produce cilia, resulting in far reaching signalling defects such as polydactyly, dorsalised neural tube, craniofacial and vascular defects often due to abnormalities in Hedgehog signal transduction. Talpid3 is absolutely required for the function of both Gli repressor and activator in the intracellular Hedgehog pathway and ciliogenesis. TALPID3 may be identified as a subcellular maker of centrosome and needed to further studied.
Protocols
Product Specific Protocols | |
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IHC protocol for TALPID3 antibody 24421-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
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EMBO Rep Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation. | ||
Cell Rep Talpid3-Mediated Centrosome Integrity Restrains Neural Progenitor Delamination to Sustain Neurogenesis by Stabilizing Adherens Junctions.
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Development Centrosomal protein CP110 controls maturation of mother centriole during cilia biogenesis. | ||
Elife TALPID3 controls centrosome and cell polarity and the human ortholog KIAA0586 is mutated in Joubert syndrome (JBTS23). | ||
Nat Commun TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating.
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