TALPID3 Polyclonal antibody

• Featured Product
• KD/KO Validated

TALPID3 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, canine and More (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 24421-1-AP

Synonyms

KIAA0586, KIAA0586 isoform, Talpid3

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Validation Data Gallery

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  IHC staining of human brain using 24421-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24421-1-AP (TALPID3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 24421-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 24421-1-AP (TALPID3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MDCK using 24421-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using TALPID3 antibody (24421-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MDCK cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:1000-1:4000

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IF: See 5 publications below
• CoIP: See 2 publications below

Product Information

The immunogen of 24421-1-AP is TALPID3 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, canine
• Cited Reactivity: human, mouse, Caenorhabditis elegans
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TALPID3 fusion protein Ag19813
• Full Name: KIAA0586
• Calculated molecular weight: 1533 aa, 169 kDa
• GenBank accession number: BC066647
• Gene symbol: KIAA0586
• Gene ID (NCBI): 9786
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

KIAA0586 also named as Talpid3 is a 1472 amino-acid protein, which belongs to Talpid3 family. Talpid3 is a gene essential for vertebrate development. Studies in chicken, mouse, zebrafish and human have shown that TALPID3 is a centrosomal protein, mutants null for TALPID3 fail to produce cilia, resulting in far reaching signalling defects such as polydactyly, dorsalised neural tube, craniofacial and vascular defects often due to abnormalities in Hedgehog signal transduction. Talpid3 is absolutely required for the function of both Gli repressor and activator in the intracellular Hedgehog pathway and ciliogenesis. TALPID3 may be identified as a subcellular maker of centrosome and needed to further studied.

Publications

Species	Application	Title
human	WB,IF	EMBO Rep; Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation.; Authors - Ezgi Odabasi
mouse	IF,CoIP	Cell Rep; Talpid3-Mediated Centrosome Integrity Restrains Neural Progenitor Delamination to Sustain Neurogenesis by Stabilizing Adherens Junctions.; Authors - Jingjing Wang ; KD Validated
human,mouse	WB	Commun Biol; FBW7 couples structural integrity with functional output of primary cilia.; Authors - Eleni Petsouki ; KD Validated
mouse		Development; Centrosomal protein CP110 controls maturation of mother centriole during cilia biogenesis.; Authors - Sharda Prasad Yadav
human	WB	EMBO Rep; Aurora Kinase A proximity map reveals centriolar satellites as regulators of its ciliary function.; Authors - Melis D Arslanhan
human	IF	J Cell Biol; A ciliopathy complex builds distal appendages to initiate ciliogenesis.; Authors - Dhivya Kumar