|Positive IHC detected in||human brain tissue, |
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
The immunogen of 24421-1-AP is TALPID3 Fusion Protein expressed in E. coli.
|Tested Reactivity||human, mouse|
|Cited Reactivity||C. elegans, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TALPID3 fusion protein Ag19813|
|Calculated molecular weight||1533 aa, 169 kDa|
|GenBank accession number||BC066647|
|Gene ID (NCBI)||9786|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
KIAA0586 also named as Talpid3 is a 1472 amino-acid protein, which belongs to Talpid3 family. Talpid3 is a gene essential for vertebrate development. Studies in chicken, mouse, zebrafish and human have shown that TALPID3 is a centrosomal protein, mutants null for TALPID3 fail to produce cilia, resulting in far reaching signalling defects such as polydactyly, dorsalised neural tube, craniofacial and vascular defects often due to abnormalities in Hedgehog signal transduction. Talpid3 is absolutely required for the function of both Gli repressor and activator in the intracellular Hedgehog pathway and ciliogenesis. TALPID3 may be identified as a subcellular maker of centrosome and needed to further studied.
Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation.
Talpid3-Mediated Centrosome Integrity Restrains Neural Progenitor Delamination to Sustain Neurogenesis by Stabilizing Adherens Junctions.
Centrosomal protein CP110 controls maturation of mother centriole during cilia biogenesis.
TALPID3 controls centrosome and cell polarity and the human ortholog KIAA0586 is mutated in Joubert syndrome (JBTS23).
TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating.