TALPID3 Polyclonal antibody

TALPID3 Polyclonal Antibody for IHC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF, CoIP,ELISA

Conjugate

Unconjugated

Cat no : 24421-1-AP

Synonyms

KIAA0586, KIAA0586 isoform, Talpid3



Tested Applications

Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 24421-1-AP is TALPID3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity Caenorhabditis elegans, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TALPID3 fusion protein Ag19813
Full Name KIAA0586
Calculated molecular weight 1533 aa, 169 kDa
GenBank accession numberBC066647
Gene symbol KIAA0586
Gene ID (NCBI) 9786
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

KIAA0586 also named as Talpid3 is a 1472 amino-acid protein, which belongs to Talpid3 family. Talpid3 is a gene essential for vertebrate development. Studies in chicken, mouse, zebrafish and human have shown that TALPID3 is a centrosomal protein, mutants null for TALPID3 fail to produce cilia, resulting in far reaching signalling defects such as polydactyly, dorsalised neural tube, craniofacial and vascular defects often due to abnormalities in Hedgehog signal transduction. Talpid3 is absolutely required for the function of both Gli repressor and activator in the intracellular Hedgehog pathway and ciliogenesis. TALPID3 may be identified as a subcellular maker of centrosome and needed to further studied.

Protocols

Product Specific Protocols
IHC protocol for TALPID3 antibody 24421-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

EMBO Rep

Centriolar satellites are required for efficient ciliogenesis and ciliary content regulation.

Authors - Ezgi Odabasi
mouseIF,CoIP

Cell Rep

Talpid3-Mediated Centrosome Integrity Restrains Neural Progenitor Delamination to Sustain Neurogenesis by Stabilizing Adherens Junctions.

Authors - Jingjing Wang
  • KD Validated
mouse

Development

Centrosomal protein CP110 controls maturation of mother centriole during cilia biogenesis.

Authors - Sharda Prasad Yadav
mouseIF

Elife

TALPID3 controls centrosome and cell polarity and the human ortholog KIAA0586 is mutated in Joubert syndrome (JBTS23).

Authors - Louise A Stephen
human,Caenorhabditis elegansWB,IF,CoIP

Nat Commun

TALPID3 and ANKRD26 selectively orchestrate FBF1 localization and cilia gating.

Authors - Hao Yan
  • KD Validated