|Positive WB detected in||SH-SY5Y cells, mouse thymus tissue, human brain tissue, mouse cerebellum tissue, mouse testis tissue, BGC-823 cells|
|Positive IP detected in||SH-SY5Y cells|
|Positive IHC detected in||mouse heart tissue, mouse brain tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||SH-SY5Y cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
18258-1-AP targets ATG13 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||ATG13 fusion protein Ag13090|
|Calculated molecular weight||57 kDa|
|Observed molecular weight||57-63 kDa|
|GenBank accession number||BC001331|
|Gene ID (NCBI)||9776|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)
|Product Specific Protocols|
|WB protocol for ATG13 antibody 18258-1-AP||Download protocol|
|IHC protocol for ATG13 antibody 18258-1-AP||Download protocol|
|IF protocol for ATG13 antibody 18258-1-AP||Download protocol|
|IP protocol for ATG13 antibody 18258-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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