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  • KD/KO Validated

ATG13 Polyclonal antibody

ATG13 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 18258-1-AP


ATG13, Autophagy related protein 13, KIAA0652

Tested Applications

Positive WB detected inSH-SY5Y cells, mouse testis tissue, BGC-823 cells, mouse thymus tissue, human brain tissue, HeLa cells, mouse cerebellum tissue, Jurkat cells, HEK-293 cells, MCF-7 cells
Positive IP detected inSH-SY5Y cells
Positive IHC detected inmouse heart tissue, mouse brain tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inSH-SY5Y cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18258-1-AP targets ATG13 in WB, IP, IF, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ATG13 fusion protein Ag13090
Full Name KIAA0652
Calculated Molecular Weight 57 kDa
Observed Molecular Weight 57-63 kDa
GenBank Accession NumberBC001331
Gene Symbol KIAA0652
Gene ID (NCBI) 9776
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)


Product Specific Protocols
WB protocol for ATG13 antibody 18258-1-APDownload protocol
IHC protocol for ATG13 antibody 18258-1-APDownload protocol
IF protocol for ATG13 antibody 18258-1-APDownload protocol
IP protocol for ATG13 antibody 18258-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols




Noncanonical autophagy promotes the visual cycle.

Authors - Kim Ji-Young JY
  • KD Validated

J Clin Invest

p53/microRNA-214/ULK1 axis impairs renal tubular autophagy in diabetic kidney disease.

Authors - Zhengwei Ma


The ALS-FTD-linked gene product, C9orf72, regulates neuronal morphogenesis via autophagy.

Authors - Wan Yun Ho

Oxid Med Cell Longev

Silencing TUFM Inhibits Development of Monocrotaline-Induced Pulmonary Hypertension by Regulating Mitochondrial Autophagy via AMPK/mTOR Signal Pathway

Authors - Ruyuan Wei

Stem Cell Res Ther

Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis.

Authors - Caixia Jin

Ecotoxicol Environ Saf

Downregulation of beclin 1 restores arsenite-induced impaired autophagic flux by improving the lysosomal function in the brain.

Authors - Hongmei Zhou