ATG13 Polyclonal antibody

• Featured Product
• KD/KO Validated

ATG13 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 18258-1-AP

Synonyms

ATG13, Autophagy related protein 13, KIAA0652

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Validation Data Gallery

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  WB analysis of SH-SY5Y using 18258-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis using 18258-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse thymus using 18258-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 18258-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18258-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 18258-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of BGC-823 using 18258-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of SH-SY5Y using 18258-1-AP

  IP Result of anti-ATG13 (IP:18258-1-AP, 4ug; Detection:18258-1-AP 1:300) with SH-SY5Y cells lysate 1600ug.

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  IHC staining of mouse heart using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18258-1-AP (ATG13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18258-1-AP (ATG13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18258-1-AP (ATG13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18258-1-AP (ATG13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse brain using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18258-1-AP (ATG13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18258-1-AP (ATG13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 18258-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using 18258-1-AP (ATG13 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse thymus tissue, human brain tissue, mouse cerebellum tissue, mouse testis tissue, BGC-823 cells
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: mouse heart tissue, mouse brain tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• CoIP: See 1 publications below

Product Information

18258-1-AP targets ATG13 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG13 fusion protein Ag13090
• Full Name: KIAA0652
• Calculated molecular weight: 57 kDa
• Observed molecular weight: 57-63 kDa
• GenBank accession number: BC001331
• Gene symbol: KIAA0652
• Gene ID (NCBI): 9776
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)

Publications

Species	Application	Title
human	WB,CoIP	Mil Med Res; Intracellular accumulation of tau inhibits autophagosome formation by activating TIA1-amino acid-mTORC1 signaling.; Authors - Meng-Zhu Li
human	WB	Mol Cell Neurosci; Elevated ATG13 in serum of patients with ME/CFS stimulates oxidative stress response in microglial cells via activation of receptor for advanced glycation end products (RAGE).; Authors - Gunnar Gottschalk ; KD Validated
rat	WB	Int Immunopharmacol; 10-Hydroxydecanoic acid inhibits LPS-induced inflammation by targeting p53 in microglial cells.; Authors - Mengmeng You
mouse	WB	Eur J Nutr; Trans-10-hydroxy-2-decenoic acid protects against LPS-induced neuroinflammation through FOXO1-mediated activation of autophagy.; Authors - Mengmeng You
human	WB	Ecotoxicol Environ Saf; Downregulation of beclin 1 restores arsenite-induced impaired autophagic flux by improving the lysosomal function in the brain.; Authors - Hongmei Zhou
mouse	WB	Stem Cell Res Ther; Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis.; Authors - Caixia Jin