ATG13 Polyclonal antibody

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  WB analysis of SH-SY5Y using 18258-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18258-1-AP

  WB result of ATG13 antibody (18258-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ATG13 transfected HeLa cells.

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  WB analysis using 18258-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18258-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 18258-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 18258-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 18258-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse cerebellum using 18258-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BGC-823 using 18258-1-AP

  BGC-823 cells were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 18258-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 18258-1-AP (ATG13 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SH-SY5Y using 18258-1-AP

  IP result of anti-ATG13 (IP:18258-1-AP, 4ug; Detection:18258-1-AP 1:300) with SH-SY5Y cells lysate 1600ug.

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  IHC staining of mouse heart using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18258-1-AP (ATG13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 18258-1-AP (ATG13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18258-1-AP (ATG13 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 18258-1-AP (ATG13 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse brain using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18258-1-AP (ATG13 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18258-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18258-1-AP (ATG13 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of SH-SY5Y using 18258-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using 18258-1-AP (ATG13 antibody) at dilution of 1:50 and CoraLite488-Conjugated Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

ATG13 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 18258-1-AP

Publications(19)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

ATG13, Autophagy related protein 13, KIAA0652

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, BGC-823 cells, HeLa cells, human brain tissue, mouse cerebellum tissue, mouse testis tissue, mouse thymus tissue, Jurkat cells, HEK-293 cells, MCF-7 cells
• Positive IP detected in: SH-SY5Y cells
• Positive IHC detected in: mouse heart tissue, mouse brain tissue, human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 17 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

18258-1-AP targets ATG13 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ATG13 fusion protein Ag13090
• Full Name: KIAA0652
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57-63 kDa
• GenBank Accession Number: BC001331
• Gene Symbol: ATG13
• Gene ID (NCBI): 9776
• RRID: AB_2130658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75143
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ATG13 is one component protein of the ULK1 complex which is required for autophagosome formation and mitophagy. ATG13 has two nutrient regulatory phosphorylation sites and the phosphorylation status of ATG13 affect regulation of autophagy by modulating enzyme activity and cellular localization of ULK1. Besides, it has been reported the nonautophagic function of ATG13 on cardiac development for ATG13-deficient embryos show myocardial growth defects.(PMID:27387056, 26801615, 26644405)

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Cell; Noncanonical autophagy promotes the visual cycle.; Authors - Kim Ji-Young JY ; KD Validated
human	WB	J Clin Invest; p53/microRNA-214/ULK1 axis impairs renal tubular autophagy in diabetic kidney disease.; Authors - Zhengwei Ma
mouse		Autophagy; The ALS-FTD-linked gene product, C9orf72, regulates neuronal morphogenesis via autophagy.; Authors - Wan Yun Ho
human	WB	Oxid Med Cell Longev; Silencing TUFM Inhibits Development of Monocrotaline-Induced Pulmonary Hypertension by Regulating Mitochondrial Autophagy via AMPK/mTOR Signal Pathway; Authors - Ruyuan Wei
mouse	WB	Stem Cell Res Ther; Chaperone-mediated autophagy plays an important role in regulating retinal progenitor cell homeostasis.; Authors - Caixia Jin
human	WB	Ecotoxicol Environ Saf; Downregulation of beclin 1 restores arsenite-induced impaired autophagic flux by improving the lysosomal function in the brain.; Authors - Hongmei Zhou