|Positive WB detected in||A431 cells, HeLa cells, HT-1080 cells, NCCIT cell|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||mouse embryo tissue, human tonsil tissue, human spleen tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human embronic stem cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11880-1-AP targets KLF4 in WB, IP, IHC, IF, chIP,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLF4 fusion protein Ag2480|
|Full Name||Kruppel-like factor 4 (gut)|
|Calculated molecular weight||504 aa, 54 kDa|
|Observed molecular weight||50-60 kDa|
|GenBank accession number||BC030811|
|Gene ID (NCBI)||9314|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The mammalian Krüppel-like transcription factor 4 (Klf4) is an evolutionarily conserved zinc finger-containing transcription factor with diverse regulatory functions in cell growth, proliferation, differentiation and embryogenesis. It also plays a significant role in the process of tumorigenesis. Klf4 is one of the key transcription factors that have been used to reprogram mouse and human fibroblasts to a pluripotent state also called induced pluripotent stem (iPS) cells. Affinity purified rabbit anti- Klf4 is for the detection of Klf4 transgene expression in the process of reprogramming. There are two isoforms of KLF4[PMID:15561714], with MW range from 50 to 60kDa,and major band is 55kDa.
Int J Biol Sci
SRC-3 protects intestine from DSS-induced colitis by inhibiting inflammation and promoting goblet cell differentiation through enhancement of KLF4 expression.
Toxicol Appl Pharmacol
Multi-walled carbon nanotubes promoted lipid accumulation in human aortic smooth muscle cells.
J Cell Biochem
SP1 regulates KLF4 via SP1 binding motif governed by DNA methylation during odontoblastic differentiation of human dental pulp cells.
J Bone Miner Res
Klf4 Promotes Dentinogenesis and Odontoblastic Differentiation via Modulation of TGF-β Signaling Pathway and Interaction With Histone Acetylation.
Biochim Biophys Acta
The histone methyltransferase SETD1A regulates thrombomodulin transcription in vascular endothelial cells.
Biochem Biophys Res Commun
Krüppel-like factor 4 synergizes with CREB to increase the activity of apolipoprotein E gene promoter in macrophages.