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  • KD/KO Validated

KLF4 Polyclonal antibody

KLF4 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, chIP,ELISA

Conjugate

Unconjugated

Cat no : 11880-1-AP

Synonyms

EZF, GKLF, KLF4, Krueppel like factor 4, Kruppel like factor 4 (gut)



Tested Applications

Positive WB detected inA431 cells, HeLa cells, HT-1080 cells, NCCIT cell
Positive IP detected inHeLa cells
Positive IHC detected inmouse embryo tissue, human tonsil tissue, human spleen tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman embronic stem cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11880-1-AP targets KLF4 in WB, IP, IHC, IF, chIP,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen KLF4 fusion protein Ag2480
Full Name Kruppel-like factor 4 (gut)
Calculated molecular weight 504 aa, 54 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC030811
Gene symbol KLF4
Gene ID (NCBI) 9314
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The mammalian Krüppel-like transcription factor 4 (Klf4) is an evolutionarily conserved zinc finger-containing transcription factor with diverse regulatory functions in cell growth, proliferation, differentiation and embryogenesis. It also plays a significant role in the process of tumorigenesis. Klf4 is one of the key transcription factors that have been used to reprogram mouse and human fibroblasts to a pluripotent state also called induced pluripotent stem (iPS) cells. Affinity purified rabbit anti- Klf4 is for the detection of Klf4 transgene expression in the process of reprogramming. There are two isoforms of KLF4[PMID:15561714], with MW range from 50 to 60kDa,and major band is 55kDa.

Protocols

Product Specific Protocols
WB protocol for KLF4 antibody 11880-1-APDownload protocol
IHC protocol for KLF4 antibody 11880-1-APDownload protocol
IP protocol for KLF4 antibody 11880-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Int J Biol Sci

SRC-3 protects intestine from DSS-induced colitis by inhibiting inflammation and promoting goblet cell differentiation through enhancement of KLF4 expression.

Authors - Wenbo Chen
humanWB

Toxicol Appl Pharmacol

Multi-walled carbon nanotubes promoted lipid accumulation in human aortic smooth muscle cells.

Authors - Haiyin Yang
mouseWB

J Cell Biochem

SP1 regulates KLF4 via SP1 binding motif governed by DNA methylation during odontoblastic differentiation of human dental pulp cells.

Authors - Zheyi Sun
mouseWB,IF,IP

J Bone Miner Res

Klf4 Promotes Dentinogenesis and Odontoblastic Differentiation via Modulation of TGF-β Signaling Pathway and Interaction With Histone Acetylation.

Authors - Huangheng Tao
humanWB,chIP

Biochim Biophys Acta

The histone methyltransferase SETD1A regulates thrombomodulin transcription in vascular endothelial cells.

Authors - Zilong Li
  • KD Validated
humanWB

Biochem Biophys Res Commun

Krüppel-like factor 4 synergizes with CREB to increase the activity of apolipoprotein E gene promoter in macrophages.

Authors - Simona Stavri