Validation Data Gallery
|Positive WB detected in||A431 cells, HT-1080 cells, NCCIT cells, HeLa cells, C2C12 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||mouse embryo tissue, human tonsil tissue, human spleen tissue, human colon tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human embronic stem cells|
|Western Blot (WB)||WB : 1:1000-1:8000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11880-1-AP targets KLF4 in WB, IP, IHC, IF, FC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLF4 fusion protein Ag2480|
|Full Name||Kruppel-like factor 4 (gut)|
|Calculated molecular weight||504 aa, 54 kDa|
|Observed molecular weight||50-60 kDa|
|GenBank accession number||BC030811|
|Gene ID (NCBI)||9314|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The mammalian Krüppel-like transcription factor 4 (Klf4) is an evolutionarily conserved zinc finger-containing transcription factor with diverse regulatory functions in cell growth, proliferation, differentiation and embryogenesis. It also plays a significant role in the process of tumorigenesis. Klf4 is one of the key transcription factors that have been used to reprogram mouse and human fibroblasts to a pluripotent state also called induced pluripotent stem (iPS) cells. Affinity purified rabbit anti- Klf4 is for the detection of Klf4 transgene expression in the process of reprogramming. There are two isoforms of KLF4 (PMID: 15561714), with MW range from 50 to 60 kDa,and major band is 55 kDa. KLF4 protein can be ubiquitin modified.
Klf4 glutamylation is required for cell reprogramming and early embryonic development in mice.
LncBRM initiates YAP1 signalling activation to drive self-renewal of liver cancer stem cells.
J Hematol Oncol
A novel lncRNA ROPM-mediated lipid metabolism governs breast cancer stem cell properties.
Fatty acid synthesis is critical for stem cell pluripotency via promoting mitochondrial fission.
J Hazard Mater
Cyanidin-3-O-glucoside ameliorates cadmium induced uterine epithelium proliferation in mice.
The rRNA m6A methyltransferase METTL5 is involved in pluripotency and developmental programs.