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  • KD/KO Validated

KLF5 Monoclonal antibody

KLF5 Monoclonal Antibody for WB, FC (Intra), ELISA
Cat No. 66850-1-Ig
Clone No.2C11C5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHeLa cells, A549 cells, NCI-H1299 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66850-1-Ig targets KLF5 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen KLF5 fusion protein Ag15403 Predict reactive species
Full Name Kruppel-like factor 5 (intestinal)
Calculated Molecular Weight 457 aa, 51 kDa
Observed Molecular Weight 51-57 kDa
GenBank Accession NumberBC042131
Gene Symbol KLF5
Gene ID (NCBI) 688
RRIDAB_2882190
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13887
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

KLF5, also known as BTEB2 and IKLF, is a member of the Krueppel-like transcription factor family. It is a transcription factor for SMemb/non-muscle myosin heavy chain-B (NMHC-B). It is a molecular marker of phenotypically modulated smooth-muscle cells. KLF5 seems to be a key element linking external stress and cardiovascular remodeling.

Protocols

Product Specific Protocols
WB protocol for KLF5 antibody 66850-1-IgDownload protocol
FC protocol for KLF5 antibody 66850-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Adv Sci (Weinh)

Positive Feedback Regulation between KLF5 and XPO1 Promotes Cell Cycle Progression of Basal like Breast Cancer

Authors - Yu Tang
  • KD Validated
humanIHC

Int J Biol Sci

Targeting the KLF5-EphA2 axis can restrain cancer stemness and overcome chemoresistance in basal-like breast cancer

Authors - Ping Zhao
humanWB

Breast Cancer Res

UCHL1 contributes to insensitivity to endocrine therapy in triple-negative breast cancer by deubiquitinating and stabilizing KLF5

Authors - Juan Li
humanWB

BMC Nephrol

Depression of LncRNA DANCR alleviates tubular injury in diabetic nephropathy by regulating KLF5 through sponge miR-214-5p

Authors - Yongling Kuang
humanWB

Cancer Lett

Energy Stress-activated AMPK Phosphorylates Snail1 and Suppresses its Stability and Oncogenic Function

Authors - Mei Li
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