KLF5 Recombinant antibody, PBS Only (Capture/Detector)

KLF5 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230509C4

Cat no : 83205-1-PBS

Synonyms

Colon krueppel-like factor, CKLF, BTE-binding protein 2, BTEB2, Basic transcription element-binding protein 2

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00155-2

  Sandwich ELISA standard curve of MP00155-2, Human KLF5 Recombinant Matched Antibody Pair - PBS only. 83205-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag15267. 83205-2-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL

• 

  Sample test of MP00155-2

  Hela and A549 cell lysates were measured. The human KLF5 concentration of detected samples was determined to be 7,055.7 pg/mL (based on a 3.2 mg/mL extract load) in Hela cell lyaste and 6,173.2 pg/mL (based on a 2.2 mg/mL extract load) in A549 cell lysate.

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  Cytometric bead array standard curve of MP00155-1

  Cytometric bead array standard curve of MP00155-1, KLF5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83205-3-PBS. Detection antibody: 83205-1-PBS. Standard: Ag15267. Range: 0.625-20 ng/mL.

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  Cytometric bead array standard curve of MP00155-2

  Cytometric bead array standard curve of MP00155-2, KLF5 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83205-1-PBS. Detection antibody: 83205-2-PBS. Standard: Ag15267. Range: 1.25-20 ng/mL.

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Product Information

83205-1-PBS targets KLF5 as part of a matched antibody pair:

MP00155-1: 83205-3-PBS capture and 83205-1-PBS detection (validated in Cytometric bead array)

MP00155-2: 83205-1-PBS capture and 83205-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: KLF5 fusion protein Ag15267
• Full Name: Kruppel-like factor 5 (intestinal)
• Calculated Molecular Weight: 457 aa, 51 kDa
• GenBank Accession Number: BC042131
• Gene Symbol: KLF5
• Gene ID (NCBI): 688
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.