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KRAS Polyclonal antibody
KRAS Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IHC, IF, FC,ELISA
Conjugate
Unconjugated
Cat no : 12063-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, A549 cells, human brain tissue, human kidney tissue, rat liver tissue, rat kidney tissue, mouse kidney tissue |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human lung cancer tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HeLa cells |
| Positive FC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| KD/KO | See 8 publications below |
| WB | See 58 publications below |
| IHC | See 12 publications below |
| IF | See 6 publications below |
| IP | See 1 publications below |
Product Information
12063-1-AP targets KRAS in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KRAS fusion protein Ag2700 |
| Full Name | v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog |
| Calculated molecular weight | 188 aa, 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC013572 |
| Gene symbol | KRAS |
| Gene ID (NCBI) | 3845 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. KRAS has two isoforms, a and b. This KRAS antibody (12063-1-AP) has weaker cross reaction to HRAS and NRAS.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for KRAS antibody 12063-1-AP | Download protocol |
| IHC protocol for KRAS antibody 12063-1-AP | Download protocol |
| IF protocol for KRAS antibody 12063-1-AP | Download protocol |
| IP protocol for KRAS antibody 12063-1-AP | Download protocol |
| FC protocol for KRAS antibody 12063-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Chem Biol HTiP: High-Throughput Immunomodulator Phenotypic Screening Platform to Reveal IAP Antagonists as Anti-cancer Immune Enhancers. | ||
Cell Death Differ MEK nuclear localization promotes YAP stability via sequestering β-TrCP in KRAS mutant cancer cells. | ||
Nat Commun Lung cancer deficient in the tumor suppressor GATA4 is sensitive to TGFBR1 inhibition. | ||
Food Chem Toxicol Final-2 targeted glycolysis mediated apoptosis and autophagy in human lung adenocarcinoma cells but failed to inhibit xenograft in nude mice. | ||
Cancer Cell Int UBN2 promotes tumor progression via the Ras/MAPK pathway and predicts poor prognosis in colorectal cancer. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Kishor (Verified Customer) (12-13-2018) | I am very much satisfied with this antibody. I got good results for cells and rat liver tissue protein.
|
FH Christopher (Verified Customer) (12-11-2018) | Antibody is much better than comparable antibodies. Because of weak expression an very low differences between K-Ras/H-Ras/N-Ras it's quite difficult to detect only K-Ras in a specific way. That is also the problem of this antibody. Please look at my attached blot. 1. Fermentas prestained marker2. PANC-1 whole cell lysate3. PANC-1/EGFP-K-Ras4. HEK293/EGFP-K-Ras5. HEK293/EGFP-H-Ras6.HEK293/EGFP-N-Ras7. 20 ng recombinant K-Ras (His-tagged)Unfortunately, K-Ras (12063-1-AP) recognizes also H-Ras and in less amounts N-Ras. Looking at endogenous K-Ras detection, you have to keep in mind, that the upper band is K-Ras and the lower one H-Ras. Because of the fact, that N-Ras is only recognized in very low amounts (probably almost not on endogenous level) this antibody is great detecting endogenous K-Ras in whole cell lysates with some little problems
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