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KRAS Antibody 36 Publications

Rabbit Polyclonal| Catalog number: 12063-1-AP

Featured Product KD/KO validated

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Reviews(2)

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

KRAS Antibody WB HeLa cells 12063-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours

KRAS Antibody IHC human lung cancer tissue 12063-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 10x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

KRAS Antibody IHC human lung cancer tissue 12063-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12063-1-AP (KRAS antibody) at dilution of 1:800 (under 40x lens) heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

KRAS Antibody IP HeLa cells 12063-1-AP

IP result of anti-KRAS(IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:1000) with HeLa cells lysate 2680 ug

KRAS Antibody IF HeLa cells 12063-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 12063-1-AP (KRAS antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

KRAS Antibody FC HeLa cells 12063-1-AP

1X10^6 HeLa cells were stained with 0.2ug KRAS antibody (12063-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, A549 cells, mouse kidney tissue, human brain tissue, human kidney tissue, HEK-293 cells, rat kidney tissue
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human lung cancer tissue, human pancreas cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:5000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:400-1:1600
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
KRAS fusion protein Ag2700
Full name:
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight:
188aa,21 kDa
Observed molecular weight:
21 kDa
GenBank accession number:
BC013572
Gene ID (NCBI):
3845
Gene symbol
KRAS
Synonyms
C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2B speicifc, Krasa, Krasb, NS3, RASK2

Background

KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. KRAS has two isoforms, a and b. This KRAS antibody (12063-1-AP) has weaker cross reaction to HRAS and NRAS.

Customer Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Share your scientific insight on this antibody

KRAS Rabbit Polyclonal antibody

Kishor (Verified Customer), 12-13-2018
Applications
Western Blot,
Primary Antibody Dilution
1:1000
No image submitted with review

No image submitted with review

Cell/Tissue Type
H69, HUCCT-1, and Rat Liver tissues
Lot Number
I am very much satisfied with this antibody. I got good results for cells and rat liver tissue protein.

KRAS Rabbit Polyclonal antibody

Christopher (Verified Customer), 12-11-2018
Applications
Western Blot,Immunofluorescence,Immunoprecipitation,
Primary Antibody Dilution
1:1000
KRAS Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:1000 dilution) in pancreatic cancer cells (PANC-1) and HEK293 overexpressing EGFP-tagged K-Ras, H-Ras and N-Ras (Cat no:12063-1-AP)

KRAS Antibody Western Blot,Immunofluorescence,Immunoprecipitation, validation (1:1000 dilution) in pancreatic cancer cells (PANC-1) and HEK293 overexpressing EGFP-tagged K-Ras, H-Ras and N-Ras (Cat no:12063-1-AP)

Cell/Tissue Type
pancreatic cancer cells (PANC-1) and HEK293 overexpressing EGFP-tagged K-Ras, H-Ras and N-Ras
Lot Number
Antibody is much better than comparable antibodies. Because of weak expression an very low differences between K-Ras/H-Ras/N-Ras it's quite difficult to detect only K-Ras in a specific way. That is also the problem of this antibody. Please look at my attached blot. 1. Fermentas prestained marker 2. PANC-1 whole cell lysate 3. PANC-1/EGFP-K-Ras 4. HEK293/EGFP-K-Ras 5. HEK293/EGFP-H-Ras 6.HEK293/EGFP-N-Ras 7. 20 ng recombinant K-Ras (His-tagged) Unfortunately, K-Ras (12063-1-AP) recognizes also H-Ras and in less amounts N-Ras. Looking at endogenous K-Ras detection, you have to keep in mind, that the upper band is K-Ras and the lower one H-Ras. Because of the fact, that N-Ras is only recognized in very low amounts (probably almost not on endogenous level) this antibody is great detecting endogenous K-Ras in whole cell lysates with some little problems

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