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KRAS Antibody 33 Publications

Rabbit Polyclonal| Catalog number: 12063-1-AP

Featured Product KD/KO validated

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Con: 39 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, FC, ELISA
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Images See all

KRAS Antibody WB HeLa cells 12063-1-AP

Various lysates were subjected to SDS PAGE followed by western blot with 12063-1-AP (KRAS antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours

KRAS Antibody IHC human lung cancer tissue 12063-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12063-1-AP( KRAS Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

KRAS Antibody IHC human lung cancer tissue 12063-1-AP

Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 12063-1-AP( KRAS Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer(pH9).

KRAS Antibody IP HeLa cells 12063-1-AP

IP result of anti-KRAS(IP:12063-1-AP, 4ug; Detection:12063-1-AP 1:1000) with HeLa cells lysate 2680 ug

KRAS Antibody IF HeLa cells 12063-1-AP

Immunofluorescent analysis of ( -20°C Ethanol) fixed HeLa cells using 12063-1-AP (KRAS antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)

KRAS Antibody FC HeLa cells 12063-1-AP

1X10^6 HeLa cells were stained with 0.2ug KRAS antibody (12063-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

Species specificity:
human, mouse, rat
Positive WB detected in:
HeLa cells, human brain tissue, human kidney tissue, mouse kidney tissue, HEK-293 cells, rat kidney tissue, mouse brain tissue, rat brain tissue, A549 cells
Positive IP detected in:
HeLa cells
Positive IHC detected in:
human lung cancer tissue, human breast cancer tissue, human colon cancer tissue
Positive IF detected in:
HeLa cells
Positive FC detected in:
HeLa cells
Recommended dilution:
WB : 1:1000-1:5000
IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
IHC : 1:50-1:500
IF : 1:50-1:500

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
KRAS fusion protein Ag2700
Full name:
v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog
Calculated molecular weight:
188aa,21 kDa
Observed molecular weight:
21 kDa
GenBank accession number:
BC013572
Gene ID (NCBI):
3845
Gene symbol
KRAS
Synonyms
C K RAS, c Ki ras, GTPase KRas, K Ras 2, K RAS2A, K RAS2B, K RAS4A, K RAS4B, KI RAS, KRAS, KRAS1, KRAS2, KRAS2B speicifc, Krasa, Krasb, NS3, RASK2

Background

KRAS, also called p21, is a member of the Ras superfamily of proteins. It is located on human chromosome 12, and contains four coding exons and a 5' non-coding exon (PMID: 12778136). KRAS is a membrane-anchored guanosine triphosphate/guanosine diphosphate (GTP/GDP)-binding protein and is widely expressed in most human cells. Like other members of the Ras family, the KRAS protein is a GTPase, and it is involved in intracellular signal transduction and mainly responsible for EGFR-signaling activation (PMID: 19117687). KRAS mutations have been found in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. KRAS has two isoforms, a and b. This KRAS antibody (12063-1-AP) has weaker cross reaction to HRAS and NRAS.


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