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Cytokeratin 13 Polyclonal antibody

Cytokeratin 13 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Cat no : 10164-2-AP

Synonyms

KRT13, Cytokeratin-13, Cytokeratin,Keratin, ck17, CK-13



Tested Applications

Positive WB detected inA431 cells, mouse skin tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman cervical cancer tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHaCaT cells, A431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10164-2-AP targets Cytokeratin 13 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 13 fusion protein Ag0217
Full Name keratin 13
Calculated Molecular Weight 50 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC002661
Gene Symbol Cytokeratin 13
Gene ID (NCBI) 3860
RRIDAB_2134679
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 13 antibody 10164-2-APDownload protocol
IHC protocol for Cytokeratin 13 antibody 10164-2-APDownload protocol
IF protocol for Cytokeratin 13 antibody 10164-2-APDownload protocol
IP protocol for Cytokeratin 13 antibody 10164-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Theranostics

Differential effect of cancer-associated fibroblast-derived extracellular vesicles on cisplatin resistance in oral squamous cell carcinoma via miR-876-3p

Authors - Soo Hyun Kang
humanIF

Elife

Chloride channels regulate differentiation and barrier functions of the mammalian airway.

Authors - Mu He
humanWB

Cell Commun Signal

Liprin-α1 modulates cancer cell signaling by transmembrane protein CD82 in adhesive membrane domains linked to cytoskeleton.

Authors - Henna Pehkonen
humanIF

Am J Respir Cell Mol Biol

Do airway epithelium air-liquid cultures represent the in vivo airway epithelium transcriptome?

Authors - Dvorak Anna A
humanIF

Stem Cells Int

Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.

Authors - Cong Li
humanIHC

Mol Vis

Expression analysis of human pterygium shows a predominance of conjunctival and limbal markers and genes associated with cell migration.

Authors - Jaworski C J CJ