Cytokeratin 13 Polyclonal antibody

Cytokeratin 13 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10164-2-AP

Synonyms

CK 13, CK13, Cytokeratin 13, K13, keratin 13, KRT13



Tested Applications

Positive WB detected inA431 cells, mouse skin tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman cervical cancer tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10164-2-AP targets Cytokeratin 13 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 13 fusion protein Ag0217
Full Name keratin 13
Calculated molecular weight 50 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC002661
Gene symbol KRT13
Gene ID (NCBI) 3860
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 13 antibody 10164-2-APDownload protocol
IHC protocol for Cytokeratin 13 antibody 10164-2-APDownload protocol
IF protocol for Cytokeratin 13 antibody 10164-2-APDownload protocol
IP protocol for Cytokeratin 13 antibody 10164-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Oncotarget

Significance of the NOR1-FOXA1/HDAC2-Slug regulatory network in epithelial-mesenchymal transition of tumor cells.

Authors - Wei Wang
humanIHC

Ann Diagn Pathol

The molecular-based differentiation of Heck's disease from its mimics including oral condyloma and white sponge nevus.

Authors - Ziv Schwartz
humanIHC

Mol Vis

Expression analysis of human pterygium shows a predominance of conjunctival and limbal markers and genes associated with cell migration.

Authors - Jaworski C J CJ
humanIF

Stem Cells Int

Pyridoxal-5'-Phosphate Promotes Immunomodulatory Function of Adipose-Derived Mesenchymal Stem Cells through Indoleamine 2,3-Dioxygenase-1 and TLR4/NF-κB Pathway.

Authors - Cong Li
humanIF

Am J Respir Cell Mol Biol

Do airway epithelium air-liquid cultures represent the in vivo airway epithelium transcriptome?

Authors - Dvorak Anna A
humanWB

Cell Commun Signal

Liprin-α1 modulates cancer cell signaling by transmembrane protein CD82 in adhesive membrane domains linked to cytoskeleton.

Authors - Henna Pehkonen