|Positive WB detected in||A431 cells, mouse skin tissue|
|Positive IP detected in||A431 cells|
|Positive IHC detected in||human cervical cancer tissue, human oesophagus tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||A431 cells|
|Western Blot (WB)||WB : 1:5000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10164-2-AP targets Cytokeratin 13 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Cytokeratin 13 fusion protein Ag0217|
|Full Name||keratin 13|
|Calculated molecular weight||50 kDa|
|Observed molecular weight||50 kDa|
|GenBank accession number||BC002661|
|Gene ID (NCBI)||3860|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Keratin 13 is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in keratin 13 gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2.
|Product Specific Protocols|
|WB protocol for Cytokeratin 13 antibody 10164-2-AP||Download protocol|
|IHC protocol for Cytokeratin 13 antibody 10164-2-AP||Download protocol|
|IF protocol for Cytokeratin 13 antibody 10164-2-AP||Download protocol|
|IP protocol for Cytokeratin 13 antibody 10164-2-AP||Download protocol|
|Click here to view our Standard Protocols|
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