Cytokeratin 16 Polyclonal antibody

Cytokeratin 16 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17265-1-AP

Synonyms

CK 16, CK16, Cytokeratin 16, K16, K1CP, keratin 16, KRT16, KRT16A, NEPPK



Tested Applications

Positive WB detected inHeLa cells, A375 cells, MCF-7 cells
Positive IHC detected inhuman oesophagus cancer tissue, human skin tissue, human cervical cancer tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

17265-1-AP targets Cytokeratin 16 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 16 fusion protein Ag10999
Full Name keratin 16
Calculated molecular weight 473 aa, 51 kDa
Observed molecular weight 48 kDa
GenBank accession numberBC039169
Gene symbol KRT16
Gene ID (NCBI) 3868
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.

Protocols

Product Specific Protocols
WB protocol for Cytokeratin 16 antibody 17265-1-APDownload protocol
IHC protocol for Cytokeratin 16 antibody 17265-1-APDownload protocol
IF protocol for Cytokeratin 16 antibody 17265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols