Cytokeratin 16 Polyclonal antibody

Cytokeratin 16 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 17265-1-AP


CK 16, CK16, Cytokeratin 16, K16, K1CP, keratin 16, KRT16, KRT16A, NEPPK

Tested Applications

Positive WB detected inHeLa cells, A375 cells, MCF-7 cells
Positive IHC detected inhuman oesophagus cancer tissue, human skin tissue, human cervical cancer tissue, mouse skin tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA431 cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

17265-1-AP targets Cytokeratin 16 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Cytokeratin 16 fusion protein Ag10999
Full Name keratin 16
Calculated Molecular Weight 473 aa, 51 kDa
Observed Molecular Weight 48 kDa
GenBank Accession NumberBC039169
Gene Symbol Cytokeratin 16
Gene ID (NCBI) 3868
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.


Product Specific Protocols
WB protocol for Cytokeratin 16 antibody 17265-1-APDownload protocol
IHC protocol for Cytokeratin 16 antibody 17265-1-APDownload protocol
IF protocol for Cytokeratin 16 antibody 17265-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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