Cytokeratin 16 Polyclonal antibody

Cytokeratin 16 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 17265-1-AP

Synonyms

CK 16, CK16, Cytokeratin 16, K16, K1CP, keratin 16, KRT16, KRT16A, NEPPK

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Validation Data Gallery

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  WB analysis using 17265-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17265-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A375 using 17265-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 17265-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human oesophagus cancer using 17265-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 17265-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 17265-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skin using 17265-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 17265-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 17265-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 17265-1-AP (Cytokeratin 16 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 17265-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 17265-1-AP (Cytokeratin 16 antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human skin using 17265-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin tissue slide using 17265-1-AP (Cytokeratin 16 antibody at dilution of 1:200 (under 40x lens).

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  IF Staining of A431 using 17265-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using Cytokeratin 16 antibody (17265-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of A431 using 17265-1-AP

  1X10^6 A431 cells were intracellularly stained with 0.2 ug Anti-Human Cytokeratin 16 (17265-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH.

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Tested Applications

• Positive WB detected in: HeLa cells, A375 cells, MCF-7 cells
• Positive IHC detected in: human oesophagus cancer tissue, human skin tissue, human cervical cancer tissue, mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: A431 cells
• Positive FC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 2 publications below

Product Information

17265-1-AP targets Cytokeratin 16 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Cytokeratin 16 fusion protein Ag10999
• Full Name: keratin 16
• Calculated Molecular Weight: 473 aa, 51 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC039169
• Gene Symbol: KRT16
• Gene ID (NCBI): 3868
• RRID: AB_2132894
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin 16 is a type I cytokeratin. It is paired with keratin 6 in a number of epithelial tissues, including nail bed, esophagus, tongue, and hair follicles.

Publications

Species	Application	Title
human,mouse	WB,IF	Phytother Res; Epigallocatechin-3-gallate promotes wound healing response in diabetic mice by activating keratinocytes and promoting re-epithelialization; Authors - Yongling Ning
mouse	IF	Front Pharmacol; Protective effect of Saussurea involucrata polysaccharide against skin dryness induced by ultraviolet radiation; Authors - Lusheng Wang