Cytokeratin 7-specific Polyclonal antibody

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• Cytokeratin 7-specific Polyclonal antibody (17513-1-AP)

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Cytokeratin 7-specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Cat No. 17513-1-AP

Publications(79)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Synonyms

Cytokeratin 7, KRT7, CK 7, CK7, CK-7

• 

  WB analysis using 17513-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of T-47D using 17513-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 17513-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 17513-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17513-1-AP (Cytokeratin 7-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 17513-1-AP

  IP result of anti-Cytokeratin 7-specific (IP:17513-1-AP, 4ug; Detection:17513-1-AP 1:1000) with HepG2 cells lysate 3600ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human bowen disease using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human bowen disease using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:20000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human ovary tumor using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). heat mediated antigen retrieved with .

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). heat mediated antigen retrieved with .

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 17513-1-AP (KRT7-Specific antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human cervical cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 17513-1-AP (KRT7-Specific antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse liver using 17513-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of mouse liver using 17513-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 17513-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HepG2 using 17513-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 17513-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 7-specific antibody (17513-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  FC experiment of HeLa using 17513-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug Cytokeratin 7-specific Polyclonal antibody (17513-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Intracellular Flow Cytometry Fixation & Permeabilization Buffer Kit (PF00019).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, T-47D cells, mouse bladder tissue, mouse lung tissue, rat bladder tissue, rat lung tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human lung cancer tissue, human bowen disease tissue, human breast cancer tissue, human cervical cancer tissue, human kidney tissue, human liver tissue, human ovary tumor tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse liver tissue
• Positive IF/ICC detected in: HeLa cells, HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:10000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17513-1-AP targets Cytokeratin 7-specific in WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: keratin 7
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 51 kDa
• GenBank Accession Number: NM_005556
• Gene Symbol: Cytokeratin 7
• Gene ID (NCBI): 3855
• RRID: AB_2134468
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08729
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. This antibody is specifically against KRT7.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Signal Transduct Target Ther; Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma.; Authors - Panpan Zhang
mouse	IHC	J Hematol Oncol; Novel roles of DC-SIGNR in colon cancer cell adhesion, migration, invasion, and liver metastasis.; Authors - Heya Na
human	IHC	Mol Cancer; DC - SIGNR by influencing the lncRNA HNRNPKP2 upregulates the expression of CXCR4 in gastric cancer liver metastasis.; Authors - Yu Zhang
mouse	IHC	Cell Res; Genomic and transcriptomic dynamics in the stepwise progression of lung adenocarcinoma.; Authors - Fangqiu Fu
human	IF	Bioact Mater; Biomimetic hydrogels with mesoscale collagen architecture for patient-derived tumor organoids culture; Authors - Jiaxin Wang
human	IF	Bioact Mater; Probiotic-inspired hybrid nanovesicles for enhancing immune checkpoint therapy efficiency via tumor immune microenvironment modulation.; Authors - Fang Wang