Cytokeratin 7-specific Polyclonal antibody

Cytokeratin 7-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

18

Cat no : 17513-1-AP

Synonyms

CK 7, CK7, Cytokeratin 7, Cytokeratin 7-specific, K2C7, K7, keratin 7, KRT7, Sarcolectin, SCL, Type II keratin Kb7

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Validation Data Gallery

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  WB analysis of A431 using 17513-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 17513-1-AP (Cytokeratin 7-specific antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 17513-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 17513-1-AP

  Immunofluorescent analysis of HepG2 cells using 17513-1-AP (Cytokeratin 7-specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IP experiment of HepG2 using 17513-1-AP

  IP Result of anti-Cytokeratin 7-specific (IP:17513-1-AP, 4ug; Detection:17513-1-AP 1:1000) with HepG2 cells lysate 3600ug.

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Tested Applications

• Positive WB detected in: A431 cells, HeLa cells, HepG2 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human lung cancer tissue, human kidney tissue, human ovary tumor tissue, human breast cancer tissue, human cervical cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IHC: See 12 publications below
• IF: See 5 publications below

Product Information

17513-1-AP targets Cytokeratin 7-specific in WB, IP, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: keratin 7
• Calculated molecular weight: 51 kDa
• Observed molecular weight: 51 kDa
• GenBank accession number: NM_005556
• Gene symbol: KRT7
• Gene ID (NCBI): 3855
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. KRT7 is a type II keratin. It is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. This antibody is specifically against KRT7.

Publications

Species	Application	Title
human	IHC	Oncol Lett; Cytokeratin expression in epidermal stem cells in skin adnexal tumors.; Authors - Xiaoqiu Zhou
human	IF	Int J Mol Med; FasL on decidual macrophages mediates trophoblast apoptosis: A potential cause of recurrent miscarriage.; Authors - Jinli Ding
	IF	Neuroreport; Noninvasive urine-derived cell lines derived from neurological genetic patients.; Authors - Zhang Qi-Jie QJ
human	IHC	Biomed Pharmacother; Leukemia inhibitory factor promote trophoblast invasion via urokinase-type plasminogen activator receptor in preeclampsia.; Authors - Qin Zheng
human	IHC	Int J Clin Exp Pathol; Pregnancy-associated plasma protein A up-regulated by progesterone promotes adhesion and proliferation of trophoblastic cells.; Authors - Jiao Wang
human	IHC	Oncol Rep; Pulmonary tumors associated with the JC virus T-antigen in a transgenic mouse model.; Authors - Noguchi Akira A