Tested Applications
| Positive WB detected in | L02 cells, pig liver tissue, mouse liver tissue, HEK-293 cells, HUVEC cells, Jurkat cells, HeLa cells, HepG2 cells |
| Positive IHC detected in | human liver cancer tissue, mouse liver tissue, human lung cancer tissue, human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
This antibody is not recommended for immunocytofluorescent assays. Mouse monoclonal antibodies of IgA isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
66301-1-Ig targets CD107b / LAMP2 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | human, mouse, pig, chicken, bovine, sheep, xenopus |
| Host / Isotype | Mouse / IgA |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0595 Product name: Recombinant human LAMP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 255-390 aa of BC002965 Sequence: NPNTTHSTGSCRSHTALLRLNSSTIKYLDFVFAVKNENRFYLKEVNISMYLVNGSVFSIANNNLSYWDAPLGSSYMCNKEQTVSVSGAFQINTFDLRVQPFNVTQGKYSTAQECSLDDDTILIPIIVGAGLSGLII Predict reactive species |
| Full Name | lysosomal-associated membrane protein 2 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 120 kDa |
| GenBank Accession Number | BC002965 |
| Gene Symbol | LAMP2 |
| Gene ID (NCBI) | 3920 |
| ENSEMBL Gene ID | ENSG00000005893 |
| RRID | AB_2881684 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Thiophilic affinity chromatograph |
| UNIPROT ID | P13473 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Lysosomal-associated membrane protein 2 (LAMP2, synonyms: LAMPB, CD107b) is a member of a family of membrane glycoproteins. This glycoprotein provides selectins with carbohydrate ligands. LAMP2 may play a role in tumor cell metastasis. It may also function in the protection, maintenance, and adhesion of the lysosome. Prior to posttranslational modification, LAMP2 is a ~45 kDa polypeptide. Mature, functional LAMP2 is extensively glycosylated with a variety of different N-linked and O-linked oligosaccharides.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD107b / LAMP2 antibody 66301-1-Ig | Download protocol |
| WB protocol for CD107b / LAMP2 antibody 66301-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD107b/LAMP2 monoclonal antibody (clone 2D3B9), catalog number 66301-1-Ig, has accumulated 204 citations across journals including Nature, Cell, Cell Metabolism, Autophagy, Nature Communications, Cell Death & Disease, Redox Biology, Molecular Cell, and Theranostics. Research fields span autophagy, ferroptosis, lysosomal biology, neurodegeneration (Alzheimer's, Parkinson's), cancer, lipid metabolism, inflammation, kidney and liver disease, virology, and immunology.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1. | ||
Cell Metab Adipocyte-derived glutathione promotes obesity-related breast cancer by regulating the SCARB2-ARF1-mTORC1 complex | ||
Cell Metab Hepatic lipid remodeling in cold exposure uncovers direct regulation of bis(monoacylglycero)phosphate lipids by phospholipase A2 group XV | ||
Immunity Deficiency of CBL and CBLB ubiquitin ligases leads to hyper T follicular helper cell responses and lupus by reducing BCL6 degradation |
Reviews
What customers say
Across 9 reviews, users report strong performance in Western blot and immunofluorescence across human, mouse, and rat samples. Most reviewers rate it 4–5 stars, praising clear signal in cell lines, brain tissue, and primary neurons. A few noted background or nonspecific bands requiring optimization, particularly in microglia and at certain dilutions. Overall, the antibody is well-received for detecting LAMP2 with reliable results in both WB and IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Boyan (Verified Customer) (06-29-2026) | good for WB
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Baptiste (Verified Customer) (06-08-2026) | Good signal but strong background.
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X (Verified Customer) (10-17-2024) | It can detect endo LAMP2 at the right MW, but also with some other bands nearby. Need optimization.
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Laura (Verified Customer) (04-08-2024) | Antibody works nicely in ICC-IF and WB in different cell lines of intestine and pancreas origin.
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LENIN (Verified Customer) (06-12-2022) | Antibody works very well for culture cells and Western blots.
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NYDIA (Verified Customer) (03-12-2022) | This antibody works great for immunofluorescence. No background. I highly recommend this product.
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Eric (Verified Customer) (03-13-2021) | I had success with some and others not. I used microglia and I did not get strong expression but in macrophages I got better bands with same dilution and treatment. Many nonspecific bands
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Tanusree (Verified Customer) (12-03-2019) | This antibody works good in western blotting analysis using mouse tissues.
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miguel (Verified Customer) (12-11-2018) | The antibody was used with an inhouse protocol for Immunohistochemistry. The results varied from good to great for both stains and imaging.
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