|Positive WB detected in||mouse lung tissue, A375 cells, A549 cells, mouse thymus tissue, rat lung tissue|
|Positive IP detected in||A549 cells|
|Positive IHC detected in||human lung tissue, human placenta tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12632-1-AP targets LAMP3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||LAMP3 fusion protein Ag3325|
|Full Name||lysosomal-associated membrane protein 3|
|Calculated molecular weight||416 aa, 44 kDa|
|Observed molecular weight||70 kDa|
|GenBank accession number||BC032940|
|Gene ID (NCBI)||27074|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
LAMP3 (lysosome-associated membrane protein 3), also known as DC-LAMP (DC-lysosome-associated membrane glycoprotein), TSC403 or CD208, is a highly glycosylated lysosomal membrane protein that belongs to the LAMP family. The mature glycoprotein has an apparent molecular mass of 70-90 kDa, which is considerably larger than its predicted mass of 44 kDa (PMID: 9768752). LAMP3 is expressed in lung type II pneumocytes, lymphoid organs and dendritic cells. It might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells. LAMP3 mRNA is up-regulated in some human cancers (PMID:9721848). LAMP3 overexpression is associated with an enhanced metastatic potential and may be a prognostic factor for cervical cancer (PMID: 16204031).
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Differences in the Immune Response of the Nonmetastatic Axillary Lymph Nodes Between Triple-Negative and Luminal A Breast Cancer Surrogate Subtypes.
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Suppression of Influenza A Virus Replication in Human Lung Epithelial Cells by Non-cytotoxic Concentrations Bafilomycin A1.
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The immune response in non-metastatic axillary lymph nodes is associated with the presence of axillary metastasis and breast cancer patient outcome.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ying (Verified Customer) (08-19-2019)
very good antibody