|Positive WB detected in||mouse lung tissue, A375 cells, A549 cells, mouse thymus tissue, rat lung tissue|
|Positive IP detected in||A549 cells|
|Positive IHC detected in||human lung tissue, human placenta tissue, human testis tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
12632-1-AP targets LAMP3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, monkey|
|Host / Isotype||Rabbit / IgG|
|Immunogen||LAMP3 fusion protein Ag3325|
|Full Name||lysosomal-associated membrane protein 3|
|Calculated molecular weight||416 aa, 44 kDa|
|Observed molecular weight||70 kDa|
|GenBank accession number||BC032940|
|Gene ID (NCBI)||27074|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
LAMP3 (lysosome-associated membrane protein 3), also known as DC-LAMP (DC-lysosome-associated membrane glycoprotein), TSC403 or CD208, is a highly glycosylated lysosomal membrane protein that belongs to the LAMP family. The mature glycoprotein has an apparent molecular mass of 70-90 kDa, which is considerably larger than its predicted mass of 44 kDa (PMID: 9768752). LAMP3 is expressed in lung type II pneumocytes, lymphoid organs and dendritic cells. It might change the lysosome function after the transfer of peptide-MHC class II molecules to the surface of dendritic cells. LAMP3 mRNA is up-regulated in some human cancers (PMID:9721848). LAMP3 overexpression is associated with an enhanced metastatic potential and may be a prognostic factor for cervical cancer (PMID: 16204031).
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Ying (Verified Customer) (08-19-2019)
very good antibody