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  • KD/KO Validated

LATS2-Specific Polyclonal antibody

LATS2-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 20276-1-AP

Synonyms

KPM, LATS2, LATS2-Specific, Warts like kinase



Tested Applications

Positive WB detected inSGC-7901 cells, mouse brain tissue, human heart tissue
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human colon tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

20276-1-AP targets LATS2-Specific in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name LATS, large tumor suppressor, homolog 2 (Drosophila)
Calculated molecular weight 120 kDa
Observed molecular weight 150 kDa
GenBank accession numberNM_014572
Gene symbol LATS2
Gene ID (NCBI) 26524
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LATS2, also named as KPM, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. LATS2 is a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. It negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. It is a negative regulator of the androgen receptor. LATS2 has a calculated molecular mass of 120 kDa and an apparent molecular mass of 150 kDa (PMID: 10871863). The antibody is specific to LATS2.

Protocols

Product Specific Protocols
WB protocol for LATS2-Specific antibody 20276-1-APDownload protocol
IHC protocol for LATS2-Specific antibody 20276-1-APDownload protocol
IF protocol for LATS2-Specific antibody 20276-1-APDownload protocol
IP protocol for LATS2-Specific antibody 20276-1-APDownload protocol
FC protocol for LATS2-Specific antibody 20276-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Cell Metab

Cholesterol Stabilizes TAZ in Hepatocytes to Promote Experimental Non-alcoholic Steatohepatitis.

Authors - Xiaobo Wang
  • KD Validated
humanWB

Cell Death Differ

The Hippo signaling component LATS2 enhances innate immunity to inhibit HIV-1 infection through PQBP1-cGAS pathway.

Authors - Tian-Sheng He
  • KO Validated
humanWB, CoIP

Clin Transl Med

Tumor-derived exosomal miR-19b-3p facilitates M2 macrophage polarization and exosomal LINC00273 secretion to promote lung adenocarcinoma metastasis via Hippo pathway.

Authors - Jing Chen
humanWB

Oncogene

TRIB3 confers radiotherapy resistance in esophageal squamous cell carcinoma by stabilizing TAZ.

Authors - Sha Zhou
humanWB

Cell Death Dis

YAP triggers the Wnt/β-catenin signalling pathway and promotes enterocyte self-renewal, regeneration and tumorigenesis after DSS-induced injury.

Authors - Feihong Deng
humanWB,IF

Cancers (Basel)

SFN Enhanced the Radiosensitivity of Cervical Cancer Cells via Activating LATS2 and Blocking Rad51/MDC1 Recruitment to DNA Damage Site.

Authors - Shiyu Wang