LATS2-Specific Polyclonal antibody

• Featured Product
• KD/KO Validated

LATS2-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugate

Unconjugated

Publications

29

Cat no : 20276-1-AP

Synonyms

KPM, LATS2, LATS2-Specific, Warts like kinase

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Validation Data Gallery

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  WB analysis of SGC-7901 using 20276-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 20276-1-AP (LATS2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 20276-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 20276-1-AP (LATS2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20276-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20276-1-AP (LATS2-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 20276-1-AP

  IP result of anti-LATS2-Specific (IP:20276-1-AP, 4ug; Detection:20276-1-AP 1:300) with HEK-293 cells lysate 2320 ug.

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  IHC staining of human liver cancer using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human colon using 20276-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 20276-1-AP

  Immunofluorescent analysis of HepG2 cells using 20276-1-AP (LATS2-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 20276-1-AP

  1X10^6 HeLa cells were stained with 0.2ug LATS2-Specific antibody (20276-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: SGC-7901 cells, mouse brain tissue, human heart tissue
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human liver cancer tissue, human colon tissue, mouse skeletal muscle tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 26 publications below
• IHC: See 7 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below

Product Information

20276-1-AP targets LATS2-Specific in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: LATS, large tumor suppressor, homolog 2 (Drosophila)
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 130-150 kDa
• GenBank Accession Number: NM_014572
• Gene Symbol: LATS2
• Gene ID (NCBI): 26524
• RRID: AB_10697657
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LATS2, also named as KPM, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. LATS2 is a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. It negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. It is a negative regulator of the androgen receptor. LATS2 has a calculated molecular mass of 120 kDa and an apparent molecular mass of 150 kDa (PMID: 10871863). The antibody is specific to LATS2.

Publications

Species	Application	Title
mouse	WB	Cell Metab; Cholesterol Stabilizes TAZ in Hepatocytes to Promote Experimental Non-alcoholic Steatohepatitis.; Authors - Xiaobo Wang ; KD Validated
human	WB	Cell Death Differ; The Hippo signaling component LATS2 enhances innate immunity to inhibit HIV-1 infection through PQBP1-cGAS pathway.; Authors - Tian-Sheng He ; KO Validated
human	WB, CoIP	Clin Transl Med; Tumor-derived exosomal miR-19b-3p facilitates M2 macrophage polarization and exosomal LINC00273 secretion to promote lung adenocarcinoma metastasis via Hippo pathway.; Authors - Jing Chen
human	WB,IHC	Oxid Med Cell Longev; miR-25 Promotes Cell Proliferation, Migration, and Invasion of Non-Small-Cell Lung Cancer by Targeting the LATS2/YAP Signaling Pathway.; Authors - Tangwei Wu
human	WB	Oncogene; TRIB3 confers radiotherapy resistance in esophageal squamous cell carcinoma by stabilizing TAZ.; Authors - Sha Zhou
human	WB	Cell Death Dis; YAP triggers the Wnt/β-catenin signalling pathway and promotes enterocyte self-renewal, regeneration and tumorigenesis after DSS-induced injury.; Authors - Feihong Deng