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- KD/KO Validated
LATS2-Specific Polyclonal antibody
LATS2-Specific Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA
Cat no : 20276-1-AP
Validation Data Gallery
|Positive WB detected in||SGC-7901 cells, mouse brain tissue, human heart tissue|
|Positive IP detected in||HEK-293 cells|
|Positive IHC detected in||human liver cancer tissue, human colon tissue, mouse skeletal muscle tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
20276-1-AP targets LATS2-Specific in WB, IP, IHC, IF, FC, CoIP, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||LATS, large tumor suppressor, homolog 2 (Drosophila)|
|Calculated molecular weight||120 kDa|
|Observed molecular weight||150 kDa|
|GenBank accession number||NM_014572|
|Gene ID (NCBI)||26524|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
LATS2, also named as KPM, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. LATS2 is a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. It negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. It is a negative regulator of the androgen receptor. LATS2 has a calculated molecular mass of 120 kDa and an apparent molecular mass of 150 kDa (PMID: 10871863). The antibody is specific to LATS2.
|Product Specific Protocols|
|WB protocol for LATS2-Specific antibody 20276-1-AP||Download protocol|
|IHC protocol for LATS2-Specific antibody 20276-1-AP||Download protocol|
|IF protocol for LATS2-Specific antibody 20276-1-AP||Download protocol|
|IP protocol for LATS2-Specific antibody 20276-1-AP||Download protocol|
|FC protocol for LATS2-Specific antibody 20276-1-AP||Download protocol|
|Click here to view our Standard Protocols|
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