LBP Polyclonal antibody

LBP Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 23559-1-AP

Synonyms

LBP

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Validation Data Gallery

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  WB analysis of mouse liver using 23559-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23559-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 23559-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 23559-1-AP (LBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 23559-1-AP

  IP result of anti-LBP(IP:23559-1-AP, 4ug; Detection:23559-1-AP 1:500) with mouse liver tissue lysate 3040 ug.

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Tested Applications

• Positive WB detected in: mouse liver tissue
• Positive IP detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

The immunogen of 23559-1-AP is LBP Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LBP fusion protein Ag13485
• Full Name: lipopolysaccharide binding protein
• Calculated molecular weight: 481 aa, 53 kDa
• Observed molecular weight: 53-60 kDa
• GenBank accession number: BC022256
• Gene symbol: LBP
• Gene ID (NCBI): 3929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LBP (lipopolysaccharide-binding protein) is an acute-phase protein, predominantly synthesized by hepatocytes. It is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. (PMID: 9665271; 11598069)

Publications

Species	Application	Title
mouse	WB	Antioxidants (Basel); Antioxidative Self-Assembling Nanoparticles Attenuate the Development of Steatohepatitis and Inhibit Hepatocarcinogenesis in Mice; Authors - Takahisa Watahiki
human	WB	Front Microbiol; Proteomics Analysis of Exosomes From Patients With Active Tuberculosis Reveals Infection Profiles and Potential Biomarkers.; Authors - Min Zhang
bovine	WB	J Proteomics; Characterization of bovine mammary gland dry secretions and their proteome from the end of lactation through day 21 of the dry period.; Authors - Timothy A Reinhardt
human,rat	IHC	Nutrition; Branched-chain amino acids protect the liver from cirrhotic injury via suppression of activation of lipopolysaccharide-binding protein, toll-like receptor 4, and signal transducer and activator of transcription 3, as well as Enterococcus faecalis translocation; Authors - Akiko Eguchi