LBP Polyclonal antibody

LBP Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP,ELISA

Conjugate

Unconjugated

Cat no : 23559-1-AP

Synonyms

LBP



Tested Applications

Positive WB detected inmouse liver tissue
Positive IP detected inmouse liver tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 23559-1-AP is LBP Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivity bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LBP fusion protein Ag13485
Full Name lipopolysaccharide binding protein
Calculated molecular weight 481 aa, 53 kDa
Observed molecular weight 53-60 kDa
GenBank accession numberBC022256
Gene symbol LBP
Gene ID (NCBI) 3929
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LBP (lipopolysaccharide-binding protein) is an acute-phase protein, predominantly synthesized by hepatocytes. It is present in normal serum at concentrations of 5 to 10 μg/ml, rising up to 200 μg/ml 24 h after induction of an acute-phase response. LBP binds to the lipid A moiety of bacterial lipopolysaccharides (LPS), a glycolipid present in the outer membrane of all Gram-negative bacteria, and acts as an affinity enhancer for CD14, facilitating its association with LPS. (PMID: 9665271; 11598069)

Protocols

Product Specific Protocols
WB protocol for LBP antibody 23559-1-APDownload protocol
IP protocol for LBP antibody 23559-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
bovineWB

J Proteomics

Characterization of bovine mammary gland dry secretions and their proteome from the end of lactation through day 21 of the dry period.

Authors - Timothy A Reinhardt