LCAT Polyclonal antibody

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  WB analysis of mouse testis using 12243-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12243-1-AP (LCAT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 12243-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12243-1-AP (LCAT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

LCAT Polyclonal Antibody for WB, ELISA

Cat No. 12243-1-AP

Publications(4)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

1-alkyl-2-acetylglycerophosphocholine esterase, EC:2.3.1.43, EC:3.1.1.47, Lecithin-cholesterol acyltransferase, Phosphatidylcholine-sterol acyltransferase

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Tested Applications

• Positive WB detected in: mouse testis tissue, HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

12243-1-AP targets LCAT in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LCAT fusion protein Ag2887
• Full Name: lecithin-cholesterol acyltransferase
• Calculated Molecular Weight: 440 aa, 50 kDa
• Observed Molecular Weight: 55 kDa
• GenBank Accession Number: BC014781
• Gene Symbol: LCAT
• Gene ID (NCBI): 3931
• RRID: AB_10638476
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P04180
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LCAT (Lecithin-cholesterol acyltransferase) is a lipoprotein-associated enzyme responsible for the synthesis of most of the plasma cholesteryl esters (CE), and as a consequence, for the maturation of high density-lipoproteins (HDL). LCAT is a 416 amino acid protein that binds to lipoproteins or is present in lipid-free form in plasma and is secreted by the liver in humans (PMID: 34256778, 26607351). Both mice and rabbits transgenic for human LCAT have marked increases in plasma HDL cholesterol and apolipoprotein (apo) A-I levels (PMID: 9054454).

Publications

Species	Application	Title
mouse		Cell Physiol Biochem; Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF.; Authors - Dandan Zhong
human	WB	Invest Ophthalmol Vis Sci; Alteration in Lysophospholipids and Converting Enzymes in Glaucomatous Optic Nerves.; Authors - Sasha M Milbeck
rat	WB	Lipids Health Dis; Chronic unpredictable mild stress combined with a high-fat diets aggravates atherosclerosis in rats.; Authors - Shuling Wang
human	WB	Heliyon; The anti-hyperlipidemia effect of Atractylodes macrocephala Rhizome increased HDL via reverse cholesterol transfer; Authors - Bo Li