LDLR Monoclonal antibody

LDLR Monoclonal Antibody for IHC, WB,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

CloneNo.

1A2A3

Cat no : 66414-1-Ig

Synonyms

FH, FHC, LDL receptor, LDLR



Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, HepG2 cells, MCF-7 cells, Raji cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:15000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

WBSee 2 publications below

Product Information

66414-1-Ig targets LDLR in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen LDLR fusion protein Ag1236
Full Name low density lipoprotein receptor
Calculated molecular weight 95 kDa
Observed molecular weight 120 kDa, 160 kDa
GenBank accession numberBC014514
Gene symbol LDLR
Gene ID (NCBI) 3949
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LDLR (low density lipoprotein receptor) is a member of the LDL receptor gene family and is involved in receptor-mediated endocytosis of specific ligands. The LDLR is a cell surface glycoprotein that scavenges LDL from the blood and regulates plasma LDL cholesterol. The cytoplasmic domain of the LDL receptor is necessary for the receptor to cluster in coated pits, which promotes the rapid endocytosis of bound LDL. The protein is highly glycosylated through N- and O-linkages and thus migrates at 100 to 160 kDa bands on SDS-PAGE.

Protocols

Product Specific Protocols
WB protocol for LDLR antibody 66414-1-IgDownload protocol
IHC protocol for LDLR antibody 66414-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Int J Mol Med

Hydrogen sulfide inhibits PCSK9 expression through the PI3K/Akt‑SREBP‑2 signaling pathway to influence lipid metabolism in HepG2 cells.

Authors - Jun Xiao
humanWB

Acta Pharmacol Sin

AMPK activator C24 inhibits hepatic lipogenesis and ameliorates dyslipidemia in HFHC diet-induced animal models.

Authors - Shui-Mei Sun