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Galectin-9 Polyclonal antibody

Galectin-9 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 17938-1-AP

Synonyms

Ecalectin, Gal 9, Galectin 9, HUAT, LGALS9, LGALS9A, Tumor antigen HOM HD 21



Tested Applications

Positive WB detected inU-937 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, HL-60 cells, SGC-7901 cells
Positive IP detected inU-937 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

17938-1-AP targets Galectin-9 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Galectin-9 fusion protein Ag12209
Full Name lectin, galactoside-binding, soluble, 9
Calculated molecular weight 323 aa, 36 kDa
Observed molecular weight 45 kDa
GenBank accession numberBC110340
Gene symbol LGALS9
Gene ID (NCBI) 3965
RRIDAB_2137233
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The galectins, formerly known as S-type lectins, are a family of β-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-9 (LGALS9) is a tandem repeat-type member of the galectin family. It has three isoforms (named galectin-9L, galectin-9M, and galectin-9S): long type of 355 amino acids, medium type of 323 amino acids, and short type of 311 amino acids. Galectin-9 is ubiquitously expressed in a variety of tissues, including lymph nodes and spleen, and overexpressed in Hodgkin disease tissue. It is involved in chemoattraction, apoptosis, and the regulation of cell differentiation and has anti-allergic effects. It has been reported that galectin-9 is a ligand for Tim-3, through which galectin-9 can induce T-helper type 1 lymphocyte (Th1) death.

Protocols

Product Specific Protocols
WB protocol for Galectin-9 antibody 17938-1-APDownload protocol
IF protocol for Galectin-9 antibody 17938-1-APDownload protocol
IP protocol for Galectin-9 antibody 17938-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Front Mol Neurosci

Galectin-9/Tim-3 pathway mediates dopaminergic neurodegeneration in MPTP-induced mouse model of Parkinson’s disease

Authors - Qinyu Peng
humanWB

J Nutr Biochem

Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression

Authors - Qi Zhang
humanWB,IHC

Front Immunol

Galectin-9/TIM-3 as a Key Regulator of Immune Response in Gliomas With Chromosome 1p/19q Codeletion.

Authors - Guanzhang Li
humanWB,IHC

Front Oncol

Prognostic Value and Biological Function of Galectins in Malignant Glioma.

Authors - Hongtao Zhu
humanWB

Stem Cell Res Ther

Mesenchymal stem cells alleviate experimental autoimmune cholangitis through immunosuppression and cytoprotective function mediated by galectin-9.

Authors - Junyu Fan
humanWB,IHC,IP

Immunotherapy

Galectin-9 and PD-L1 antibody blockade combination therapy inhibits tumour progression in pancreatic cancer

Authors - Enliang Li