Galectin-9 Polyclonal antibody

• Featured Product
• KD/KO Validated

Galectin-9 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 17938-1-AP

Synonyms

Ecalectin, Gal 9, Galectin 9, HUAT, LGALS9, LGALS9A, Tumor antigen HOM HD 21

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Validation Data Gallery

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  WB analysis of NIH/3T3 using 17938-1-AP

  WB result of Galectin 9 antibody (17938-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Galectin 9 transfected NIH/3T3 cells.

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  WB analysis of U-937 using 17938-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 17938-1-AP (Galectin 9 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17938-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17938-1-AP (Galectin 9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 17938-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 17938-1-AP (Galectin 9 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HL-60 using 17938-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 17938-1-AP (Galectin 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SGC-7901 using 17938-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 17938-1-AP (Galectin 9 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of SGC-7901 using 17938-1-AP

  IP Result of anti-Galectin 9 (IP:17938-1-AP, 4ug; Detection:17938-1-AP 1:500) with SGC-7901 cells lysate 2400ug.

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  IF Staining of HeLa using 17938-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 17938-1-AP (Galectin 9 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: U-937 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, HL-60 cells, SGC-7901 cells
• Positive IP detected in: SGC-7901 cells
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 6 publications below
• IHC: See 3 publications below

Product Information

17938-1-AP targets Galectin-9 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Galectin-9 fusion protein Ag12209
• Full Name: lectin, galactoside-binding, soluble, 9
• Calculated molecular weight: 323 aa, 36 kDa
• Observed molecular weight: 45 kDa
• GenBank accession number: BC110340
• Gene symbol: LGALS9
• Gene ID (NCBI): 3965
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The galectins, formerly known as S-type lectins, are a family of β-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-9 (LGALS9) is a tandem repeat-type member of the galectin family. It has three isoforms (named galectin-9L, galectin-9M, and galectin-9S): long type of 355 amino acids, medium type of 323 amino acids, and short type of 311 amino acids. Galectin-9 is ubiquitously expressed in a variety of tissues, including lymph nodes and spleen, and overexpressed in Hodgkin disease tissue. It is involved in chemoattraction, apoptosis, and the regulation of cell differentiation and has anti-allergic effects. It has been reported that galectin-9 is a ligand for Tim-3, through which galectin-9 can induce T-helper type 1 lymphocyte (Th1) death.

Publications

Species	Application	Title
mouse	WB	Front Mol Neurosci; Galectin-9/Tim-3 pathway mediates dopaminergic neurodegeneration in MPTP-induced mouse model of Parkinson’s disease; Authors - Qinyu Peng
human	WB	J Nutr Biochem; Isothiocyanates attenuate immune checkpoint blockage therapy in gastric cancer via induction of PD-L1 expression; Authors - Qi Zhang
human	WB,IHC	Front Immunol; Galectin-9/TIM-3 as a Key Regulator of Immune Response in Gliomas With Chromosome 1p/19q Codeletion.; Authors - Guanzhang Li
human	WB,IHC	Front Oncol; Prognostic Value and Biological Function of Galectins in Malignant Glioma.; Authors - Hongtao Zhu
human	WB	Stem Cell Res Ther; Mesenchymal stem cells alleviate experimental autoimmune cholangitis through immunosuppression and cytoprotective function mediated by galectin-9.; Authors - Junyu Fan
		Int J Oncol; Role of downregulation of galectin-9 in the tumorigenesis of gastric cancer.; Authors - Jiaxing Yang