|Positive WB detected in||U-937 cells, HeLa cells, HepG2 cells, NIH/3T3 cells, HL-60 cells, SGC-7901 cells|
|Positive IP detected in||SGC-7901 cells|
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
17938-1-AP targets Galectin-9 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Galectin-9 fusion protein Ag12209|
|Full Name||lectin, galactoside-binding, soluble, 9|
|Calculated molecular weight||323 aa, 36 kDa|
|Observed molecular weight||45 kDa|
|GenBank accession number||BC110340|
|Gene ID (NCBI)||3965|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
The galectins, formerly known as S-type lectins, are a family of β-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-9 (LGALS9) is a tandem repeat-type member of the galectin family. It has three isoforms (named galectin-9L, galectin-9M, and galectin-9S): long type of 355 amino acids, medium type of 323 amino acids, and short type of 311 amino acids. Galectin-9 is ubiquitously expressed in a variety of tissues, including lymph nodes and spleen, and overexpressed in Hodgkin disease tissue. It is involved in chemoattraction, apoptosis, and the regulation of cell differentiation and has anti-allergic effects. It has been reported that galectin-9 is a ligand for Tim-3, through which galectin-9 can induce T-helper type 1 lymphocyte (Th1) death.
Critical role of Tim-3 mediated autophagy in chronic stress induced immunosuppression.
Int J Oncol
Role of downregulation of galectin-9 in the tumorigenesis of gastric cancer.
Stem Cell Res Ther
Mesenchymal stem cells alleviate experimental autoimmune cholangitis through immunosuppression and cytoprotective function mediated by galectin-9.