Tested Applications
| Positive WB detected in | U-937 cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-937 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17938-1-AP targets LGALS9/Galectin-9 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12209 Product name: Recombinant human LGALS9, Galectin-9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-323 aa of BC110340 Sequence: MAFSGSQAPYLSPAVPFSGTIQGGLQDGLQITVNGTVLSSSGTRFAVNFQTGFSGNDIAFHFNPRFEDGGYVVCNTRQNGSWGPEERKTHMPFQKGMPFDLCFLVQSSDFKVMVNGILFVQYFHRVPFHRVDTISVNGSVQLSYISFQPPGVWPANPAPITQTVIHTVQSAPGQMFSTPAIPPMMYPHPAYPMPFITTILGGLYPSKSILLSGTVLPSAQRFHINLCSGNHIAFHLNPRFDENAVVRNTQIDNSWGSEERSLPRKMPFVRGQSFSVWILCEAHCLKVAVDGQHLFEYYHRLRNLPTINRLEVGGDIQLTHVQT Predict reactive species |
| Full Name | lectin, galactoside-binding, soluble, 9 |
| Calculated Molecular Weight | 323 aa, 36 kDa |
| Observed Molecular Weight | 34-40 kDa |
| GenBank Accession Number | BC110340 |
| Gene Symbol | Galectin-9 |
| Gene ID (NCBI) | 3965 |
| RRID | AB_2137233 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O00182 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
The galectins, formerly known as S-type lectins, are a family of β-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Galectin-9 (LGALS9) is a tandem repeat-type member of the galectin family. It has three isoforms (named galectin-9L, galectin-9M, and galectin-9S): long type of 355 amino acids, medium type of 323 amino acids, and short type of 311 amino acids. Galectin-9 is ubiquitously expressed in a variety of tissues, including lymph nodes and spleen, and overexpressed in Hodgkin disease tissue. It is involved in chemoattraction, apoptosis, and the regulation of cell differentiation and has anti-allergic effects. It has been reported that galectin-9 is a ligand for Tim-3, through which galectin-9 can induce T-helper type 1 lymphocyte (Th1) death.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LGALS9/Galectin-9 antibody 17938-1-AP | Download protocol |
| IHC protocol for LGALS9/Galectin-9 antibody 17938-1-AP | Download protocol |
| WB protocol for LGALS9/Galectin-9 antibody 17938-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LGALS9/Galectin-9 Polyclonal antibody (Cat# 17938-1-AP) has 34 citations. They appear in journals including Mol Cancer, Neuron, Cell Death Differ, Cell Rep Med, Acta Pharm Sin B, Front Immunol, J Biol Chem, Cell Mol Life Sci, Stem Cell Res Ther, Int J Oncol, Cancer Genet, Sci Rep, Cell Signal, Clin Transl Med, Front Mol Neurosci, Parasit Vectors, Viruses, and others. Research fields span oncology and immunology (tumor microenvironment, immune checkpoints), neurology (Parkinson's disease, neuroinflammation), infectious disease, and liver disease.
| Species | Application | Title |
|---|---|---|
Mol Cancer Targeting the CD47-HCK-LGALS9 axis disrupts proliferation-immunosuppression coupling in early-stage endometrial cancer. | ||
Neuron C5aR1+ microglia exacerbate neuroinflammation and cerebral edema in acute brain injury | ||
Acta Pharm Sin B PKCα inhibitors promote breast cancer immune evasion by maintaining PD-L1 stability | ||
Cell Rep Med Lnc-H19-derived protein shapes the immunosuppressive microenvironment of glioblastoma | ||
Cell Death Differ Galectin-9 activates microglial asparagine endopeptidase and promotes α-synuclein pathology in Parkinson's disease. | ||
Research (Wash D C) Single-Cell RNA Transcriptomics and Multi-omics Analyses Reveal the Clinical Effects of Acupuncture on Methadone Reduction |
