LHCGR Polyclonal antibody

LHCGR Polyclonal Antibody for FC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, FC, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 19968-1-AP

Synonyms

LCGR, LGR2, LH/CG R, LH/CGR, LHCGR, LHR, LHRHR, LSH R, Luteinizing Hormone Receptor

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Validation Data Gallery

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  WB analysis using 19968-1-AP

  Unboiled mouse testis tissue was subjected to SDS PAGE followed by western blot with 19968-1-AP (LHCGR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 19968-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19968-1-AP (LHCGR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 19968-1-AP

  IP Result of anti-LHCGR (IP:19968-1-AP, 4ug; Detection:19968-1-AP 1:300) with mouse testis tissue lysate 4000ug.

• 

  FC experiment of Jurkat using 19968-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human LHCGR (19968-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive WB detected in: unboiled mouse testis tissue, mouse ovary tissue
• Positive IP detected in: mouse testis tissue
• Positive FC detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:150-1:600 for WB

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 17 publications below
• IHC: See 3 publications below

Product Information

19968-1-AP targets LHCGR in WB, IP, IHC, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: LHCGR
• Calculated molecular weight: 79 kDa
• Observed molecular weight: 90-100 kDa
• GenBank accession number: NM_000233
• Gene symbol: LHCGR
• Gene ID (NCBI): 3973
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lutropin-choriogonadotropic hormone receptor (LHCGR, also known as LHR or LSHR) belongs to the rhodopsin-like family of G protein-coupled receptors (GPCRs) and plays a crucial role in the regulation of gonadal functions by stimulating steroidogenesis in response to luteinizing hormone (LH) or hCG (PMID: 15866423). Mutations in LHCGR gene result in familial male-limited precocious puberty, Leydig cell hypoplasia, and empty follicle syndrome (PMID: 30711030). LHCGR protein consists of 699 amino acids with a calculated molecular weight of 79 kDa. Due to massive glycosylation, the molecular mass of the mature LHCGR is higher (85-100 kDa) than the predicted molecular protein mass of 79 kDa (PMID: 23686864; 15866423; 29075189; 15969756).

Publications

Species	Application	Title
mouse	WB	Part Fibre Toxicol; Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.; Authors - Haibo Jin
human	WB	Am J Pathol; Amyloid Precursor Protein Overexpression in Down Syndrome Trophoblast Reduces Cell Invasiveness and Interferes with Syncytialization.; Authors - Oscar Gee Wan Wong
mouse	WB	PLoS One; The RNA-Binding Protein, ZFP36L2, Influences Ovulation and Oocyte Maturation.; Authors - Christopher B Ball
mouse	WB	Chemosphere; PMID: 28081510; Authors - Xiaoyang Wang
mouse	WB,IHC	Front Endocrinol (Lausanne); Downregulation of LHCGR Attenuates COX-2 Expression and Induces Luteinized Unruptured Follicle Syndrome in Endometriosis.; Authors - Ting Geng
mouse	WB, IHC	Nutr Metab (Lond); Effects of increasing intake of soybean oil on synthesis of testosterone in Leydig cells.; Authors - Yu Su

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