LHCGR Polyclonal antibody

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  WB analysis using 19968-1-AP

  Unboiled mouse testis tissue was subjected to SDS PAGE followed by western blot with 19968-1-AP (LHCGR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse ovary using 19968-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 19968-1-AP (LHCGR antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 19968-1-AP

  IP result of anti-LHCGR (IP:19968-1-AP, 4ug; Detection:19968-1-AP 1:300) with mouse testis tissue lysate 4000ug.

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  IF Staining of mouse ovary using 19968-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse ovary tissue using LHCGR antibody (19968-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• Featured Product
• KD/KO Validated

LHCGR Polyclonal Antibody for WB, IF-P, IP, ELISA

Cat No. 19968-1-AP

Publications(33)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF-P, IP, ELISA

Synonyms

LHR, LCGR, LGR2, LH R, LH receptor

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Tested Applications

• Positive WB detected in: unboiled mouse testis tissue, mouse ovary tissue
• Positive IP detected in: mouse testis tissue
• Positive IF-P detected in: mouse ovary tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 33 publications below
• IHC: See 5 publications below

Product Information

19968-1-AP targets LHCGR in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, sheep
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: LHCGR
• Calculated Molecular Weight: 79 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: NM_000233
• Gene Symbol: LHCGR
• Gene ID (NCBI): 3973
• RRID: AB_10697685
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P22888
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lutropin-choriogonadotropic hormone receptor (LHCGR, also known as LHR or LSHR) belongs to the rhodopsin-like family of G protein-coupled receptors (GPCRs). It plays a crucial role in the regulation of gonadal functions by stimulating steroidogenesis in response to luteinizing hormone (LH) or hCG (PMID: 15866423). Mutations in the LHCGR gene result in familial male-limited precocious puberty, Leydig cell hypoplasia, and empty follicle syndrome (PMID: 30711030). LHCGR protein consists of 699 amino acids with a calculated molecular weight of 79 kDa. Due to massive glycosylation, the molecular mass of the mature LHCGR is higher (85-100 kDa) than the predicted molecular protein mass of 79 kDa (PMID: 23686864; 15866423; 29075189; 15969756).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	J Hazard Mater; GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP facilitates iodoacetic acid-mediated testosterone decline; Authors - Li Mou
mouse	WB	Environ Pollut; Chronic exposure to polystyrene microplastics induced LHR reduction and decreased testosterone levels through NF-κB pathway; Authors - Haibo Jin
mouse	WB	Part Fibre Toxicol; Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.; Authors - Haibo Jin
mouse	WB	Int J Nanomedicine; La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice.; Authors - Lu Yuan
pig	WB	Biol Res; Unraveling the impact of hyperleptinemia on female reproduction: insights from transgenic pig model; Authors - Muhammad Ameen Jamal
human	WB	Reprod Biol Endocrinol; miR-6881-3p contributes to diminished ovarian reserve by regulating granulosa cell apoptosis by targeting SMAD4; Authors - Wenhan Ju