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LHCGR Polyclonal antibody
LHCGR Polyclonal Antibody for WB, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, IF-P, IP, ELISA
Conjugate
Unconjugated
Cat no : 19968-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | unboiled mouse testis tissue, mouse ovary tissue |
Positive IP detected in | mouse testis tissue |
Positive IF-P detected in | mouse ovary tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 2 publications below |
WB | See 26 publications below |
IHC | See 4 publications below |
Product Information
19968-1-AP targets LHCGR in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Peptide |
Full Name | LHCGR |
Calculated Molecular Weight | 79 kDa |
Observed Molecular Weight | 90-100 kDa |
GenBank Accession Number | NM_000233 |
Gene Symbol | LHCGR |
Gene ID (NCBI) | 3973 |
RRID | AB_10697685 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Lutropin-choriogonadotropic hormone receptor (LHCGR, also known as LHR or LSHR) belongs to the rhodopsin-like family of G protein-coupled receptors (GPCRs). It plays a crucial role in the regulation of gonadal functions by stimulating steroidogenesis in response to luteinizing hormone (LH) or hCG (PMID: 15866423). Mutations in the LHCGR gene result in familial male-limited precocious puberty, Leydig cell hypoplasia, and empty follicle syndrome (PMID: 30711030). LHCGR protein consists of 699 amino acids with a calculated molecular weight of 79 kDa. Due to massive glycosylation, the molecular mass of the mature LHCGR is higher (85-100 kDa) than the predicted molecular protein mass of 79 kDa (PMID: 23686864; 15866423; 29075189; 15969756).
Protocols
Product Specific Protocols | |
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WB protocol for LHCGR antibody 19968-1-AP | Download protocol |
IF protocol for LHCGR antibody 19968-1-AP | Download protocol |
IP protocol for LHCGR antibody 19968-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Part Fibre Toxicol Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway. | ||
Int J Nanomedicine La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice. | ||
Reprod Biol Endocrinol miR-6881-3p contributes to diminished ovarian reserve by regulating granulosa cell apoptosis by targeting SMAD4 | ||
Am J Pathol Amyloid Precursor Protein Overexpression in Down Syndrome Trophoblast Reduces Cell Invasiveness and Interferes with Syncytialization. | ||