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LHCGR Polyclonal antibody

LHCGR Polyclonal Antibody for FC, IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 19968-1-AP

Synonyms

LCGR, LGR2, LH/CG R, LH/CGR, LHCGR, LHR, LHRHR, LSH R, Luteinizing Hormone Receptor



Tested Applications

Positive WB detected inunboiled mouse testis tissue, mouse ovary tissue
Positive IP detected inmouse testis tissue
Positive IF detected inmouse ovary tissue
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

19968-1-AP targets LHCGR in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name LHCGR
Calculated molecular weight 79 kDa
Observed molecular weight 90-100 kDa
GenBank accession numberNM_000233
Gene symbol LHCGR
Gene ID (NCBI) 3973
RRIDAB_10697685
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lutropin-choriogonadotropic hormone receptor (LHCGR, also known as LHR or LSHR) belongs to the rhodopsin-like family of G protein-coupled receptors (GPCRs) and plays a crucial role in the regulation of gonadal functions by stimulating steroidogenesis in response to luteinizing hormone (LH) or hCG (PMID: 15866423). Mutations in LHCGR gene result in familial male-limited precocious puberty, Leydig cell hypoplasia, and empty follicle syndrome (PMID: 30711030). LHCGR protein consists of 699 amino acids with a calculated molecular weight of 79 kDa. Due to massive glycosylation, the molecular mass of the mature LHCGR is higher (85-100 kDa) than the predicted molecular protein mass of 79 kDa (PMID: 23686864; 15866423; 29075189; 15969756).

Protocols

Product Specific Protocols
WB protocol for LHCGR antibody 19968-1-APDownload protocol
IF protocol for LHCGR antibody 19968-1-APDownload protocol
IP protocol for LHCGR antibody 19968-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Part Fibre Toxicol

Chronic exposure to polystyrene microplastics induced male reproductive toxicity and decreased testosterone levels via the LH-mediated LHR/cAMP/PKA/StAR pathway.

Authors - Haibo Jin
mouseWB

Int J Nanomedicine

La2O3 Nanoparticles Induce Reproductive Toxicity Mediated by the Nrf-2/ARE Signaling Pathway in Kunming Mice.

Authors - Lu Yuan
humanWB

Am J Pathol

Amyloid Precursor Protein Overexpression in Down Syndrome Trophoblast Reduces Cell Invasiveness and Interferes with Syncytialization.

Authors - Oscar Gee Wan Wong
mouseWB

PLoS One

The RNA-Binding Protein, ZFP36L2, Influences Ovulation and Oocyte Maturation.

Authors - Christopher B Ball
mouseWB

Chemosphere

PMID: 28081510

Authors - Xiaoyang Wang
mouseWB,IHC

Front Endocrinol (Lausanne)

Downregulation of LHCGR Attenuates COX-2 Expression and Induces Luteinized Unruptured Follicle Syndrome in Endometriosis.

Authors - Ting Geng