LIMK2 Polyclonal antibody

LIMK2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 12350-1-AP


LIM domain kinase 2, LIMK 2, LIMK2

Tested Applications

Positive WB detected inNIH/3T3 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12350-1-AP targets LIMK2 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIMK2 fusion protein Ag3011
Full Name LIM domain kinase 2
Calculated Molecular Weight 686 aa, 78 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC013051
Gene Symbol LIMK2
Gene ID (NCBI) 3985
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The family of LIM kinase (LIMK) includes two similar proteins, LIMK1 and LIMK2, that share overall identity of 53% with 75%, 55%, and 42% amino acid identity in the LIM, kinase, and PDZ domains, respectively. LIMK2 regulates actin cytoskeletal reorganization through phosphorylating and inactivating cofilin, an actin-depolymerizing factor of actin filaments. It also may associate with gamma-tubulin and play a role in mitotic spindle assembly. High levels of LIMK2 are evident in uterus, thymus, and spleen whereas lower levels are observed in brain, heart, liver, stomach, skeletal muscle, and testes according to the results of western blotting(PMID:16399995). It has 3 isoforms produced by alternative splicing of the molecular weight of 70-78 kDa. It can be detected a band of 47 kDa or 50-55 kDa in the testes because the antibody is recognizing the testis-specific variant of LIMK2 and the this difference could be due to species variation(PMID:17928627).


Product Specific Protocols
WB protocol for LIMK2 antibody 12350-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Adv Healthc Mater

Modification of PLGA Scaffold by MSC-Derived Extracellular Matrix Combats Macrophage Inflammation to Initiate Bone Regeneration via TGF-β-Induced Protein.

Authors - Moyuan Deng

J Cachexia Sarcopenia Muscle

RhoA/ROCK2 signalling is enhanced by PDGF-AA in fibro-adipogenic progenitor cells: implications for Duchenne muscular dystrophy.

Authors - Esther Fernández-Simón


NUDT21 inhibits bladder cancer progression through ANXA2 and LIMK2 by alternative polyadenylation.

Authors - Ming Xiong

Cell Death Dis

Imbalanced LIMK1 and LIMK2 expression leads to human colorectal cancer progression and metastasis via promoting β-catenin nuclear translocation.

Authors - Yue Zhang

PLoS One

ECM-dependent HIF induction directs trophoblast stem cell fate via LIMK1-mediated cytoskeletal rearrangement.

Authors - Choi Hwa J HJ

Mol Biotechnol

Stratifin (SFN) Regulates Cervical Cancer Cell Proliferation, Apoptosis, and Cytoskeletal Remodeling and Metastasis Progression Through LIMK2/Cofilin Signaling

Authors - Naiyi Du