LIN28A Polyclonal antibody

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  WB analysis using 11724-1-AP

  Various lysate were subjected to SDS PAGE followed by western blot with 11724-1-AP (LIN28 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT cells using 11724-1-AP

  WB data of LIN28 antibody (11724-1-AP, 1:500) with si-control and si-LIN28 transfected NCCIT cells.

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  IP experiment of K-562 using 11724-1-AP

  IP result of anti-LIN28 (IP:11724-1-AP, 4ug; Detection:11724-1-AP 1:2000) with K-562 cells lysate 1360 ug.

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  IHC staining of mouse testis using 11724-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11724-1-AP (LIN28 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 11724-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11724-1-AP (LIN28 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human embronic stem cells using 11724-1-AP

  Confocal immunofluorescent analysis of human embronic stem cells with 11724-1-AP at dilution of 1:200. The PE shows staining with 11724-1-AP/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI. (10X).

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  IF Staining of human embronic stem cells using 11724-1-AP

  Confocal immunofluorescent analysis of human embronic stem cells with 11724-1-AP at dilution of 1:400. The PE shows staining with 11724-1-AP/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI. (20X).

• Featured Product
• KD/KO Validated

LIN28A Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 11724-1-AP

Publications(57)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Synonyms

Protein lin 28 homolog A, Lin-28A, LIN28, Lin 28A, LIN 28

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Tested Applications

• Positive WB detected in: K-562 cells, NCCIT cells, mouse embryo tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: mouse testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: human embronic stem cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 29 publications below
• IHC: See 18 publications below
• IF: See 14 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Product Information

11724-1-AP targets LIN28A in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIN28A fusion protein Ag2312
• Full Name: lin-28 homolog (C. elegans)
• Calculated Molecular Weight: 209 aa, 23 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC028566
• Gene Symbol: LIN28
• Gene ID (NCBI): 79727
• RRID: AB_2135039
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H9Z2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell[PMID: 20139967]. LIN28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic mRNA-binding protein that binds to and enhances the translation of the IGF2 mRNA[PMID: 21057460]. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells[PMID: 22078496]. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against full length LIN28 of human origin. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IHC	Nature; A role for Lin28 in primordial germ-cell development and germ-cell malignancy.; Authors - West Jason A JA
human,mouse	WB,IHC	Nat Genet; Lin28 promotes transformation and is associated with advanced human malignancies.; Authors - Viswanathan Srinivas R SR
mouse	IHC	Nat Genet; Lin28a transgenic mice manifest size and puberty phenotypes identified in human genetic association studies.; Authors - Zhu Hao H
human	WB,IF	Nat Methods; A nonviral minicircle vector for deriving human iPS cells.; Authors - Jia Fangjun F
human	IF	Cell Stem Cell; Human iPSC-Derived Neural Progenitors Are an Effective Drug Discovery Model for Neurological mtDNA Disorders.; Authors - Carmen Lorenz
mouse	IHC	Cell Res; Ythdc2 is an N(6)-methyladenosine binding protein that regulates mammalian spermatogenesis.; Authors - Phillip J Hsu