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LIN28 Antibody

LIN28 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11724-1-AP

Synonyms

CSDD1, LIN 28, lin 28 homolog (C. elegans), Lin 28A, LIN28, Lin28A, Protein lin 28 homolog A, ZCCHC1



Tested Applications

Positive WB detected inNCCIT cell, NCCIT cells, K-562 cells, mouse embryo tissue
Positive IP detected inK-562 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman embronic stem cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11724-1-AP targets LIN28 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity dog, human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LIN28 fusion protein Ag2312
Full Name lin-28 homolog (C. elegans)
Calculated molecular weight 209aa,23 kDa
Observed molecular weight 28 kDa
GenBank accession number BC028566
Gene symbol LIN28
Gene ID (NCBI) 79727
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell[PMID: 20139967]. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna[PMID: 21057460]. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells[PMID: 22078496]. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against full length LIN28 of human origin. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.

Protocols

Product Specific Protocols
WB protocol for LIN28 antibody 11724-1-APDownload protocol
IHC protocol for LIN28 antibody 11724-1-APDownload protocol
IP protocol for LIN28 antibody 11724-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Clin Endocrinol (Oxf)

Expression of LIN28 and its regulatory microRNAs in adult adrenocortical cancer.

Authors - André M Faria
humanIHC

Pediatr Dev Pathol

Immunohistochemical expression of embryonic stem cell markers in malignant rhabdoid tumors.

Authors - Deisch Jeremy J
human

Pediatr Surg Int

RNA-binding protein LIN28 is a sensitive marker of pediatric yolk sac tumors.

Authors - Shaoguang Feng
humanWB

Cell J

Rational Development of A Polycistronic Plasmid with A CpG-Free Bacterial Backbone as A Potential Tool for Direct Reprogramming.

Authors - Kianoush Dormiani
humanIHC

Pathol Res Pract

Expression of RNA-binding protein LIN28 in classic gastric hepatoid carcinomas, gastric fetal type gastrointestinal adenocarcinomas, and hepatocellular carcinomas: An immunohistochemical study with comparison to SALL4, alpha-fetoprotein, glypican-3, and Hep Par1.

Authors - Ming Zhao
dogWB,IHC,IF

Anim Reprod Sci

Analysis of putative biomarkers of undifferentiated spermatogonia in dog testis.

Authors - Won-Young Lee