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LIN28 Antibody 36 Publications

Rabbit Polyclonal| Catalog number: 11724-1-AP

Featured Product KD/KO validated

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Con: 47 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

LIN28 Antibody IP K-562 cells 11724-1-AP

IP Result of anti-LIN28 (IP:11724-1-AP, 3ug; Detection:11724-1-AP 1:500) with K-562 cells lysate 1000ug.

LIN28 Antibody IHC human prostate cancer tissue 11724-1-AP

Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11724-1-AP(LIN28 antibody) at dilution of 1:50 (under 40x lens)

LIN28 Antibody IF human embronic stem cells 11724-1-AP

Confocal immunofluorescent analysis of human embronic stem cells with 11724-1-AP at dilution of 1:200. The PE shows staining with 11724-1-AP/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI.(10X)

LIN28 Antibody IF human embronic stem cells 11724-1-AP

Confocal immunofluorescent analysis of human embronic stem cells with 11724-1-AP at dilution of 1:400. The PE shows staining with 11724-1-AP/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI. (20X)

LIN28 Antibody WB NCCIT cell 11724-1-AP

NCCIT cell were subjected to SDS PAGE followed by western blot with 11724-1-AP( LIN28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

Species specificity:
human, mouse, rat, dog
Positive WB detected in:
NCCIT cell, K-562 cells, mouse embryo tissue, s
Positive IP detected in:
K-562 cells
Positive IHC detected in:
human prostate cancer tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
human embronic stem cells
Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:200
IF : 1:20-1:200
Sample-dependent, check data in Validation tab

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
LIN28 fusion protein Ag2312
Full name:
lin-28 homolog (C. elegans)
Calculated molecular weight:
209aa,23 kDa
Observed molecular weight:
28 kDa
GenBank accession number:
BC028566
Gene ID (NCBI):
79727
Gene symbol
LIN28
Synonyms
CSDD1, LIN 28, lin 28 homolog (C. elegans), Lin 28A, LIN28, Lin28A, Protein lin 28 homolog A, ZCCHC1

Background

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell[PMID: 20139967]. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna[PMID: 21057460]. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells[PMID: 22078496]. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against full length LIN28 of human origin. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.


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