LIN28 Polyclonal antibody

• Featured Product
• KD/KO Validated

LIN28 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

41

Cat no : 11724-1-AP

Synonyms

CSDD1, LIN 28, lin 28 homolog (C. elegans), Lin 28A, LIN28, Lin28A, Protein lin 28 homolog A, ZCCHC1

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Validation Data Gallery

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  WB analysis of NCCIT cells using 11724-1-AP

  WB data of LIN28 antibody (11724-1-AP, 1:500) with si-control and si-LIN28 transfected NCCIT cells.

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  IP experiment of K-562 using 11724-1-AP

  IP Result of anti-LIN28 (IP:11724-1-AP, 3ug; Detection:11724-1-AP 1:500) with K-562 cells lysate 1000ug.

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  IHC staining of human prostate cancer using 11724-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11724-1-AP (LIN28 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of human embronic stem cells using 11724-1-AP

  Confocal immunofluorescent analysis of human embronic stem cells with 11724-1-AP at dilution of 1:200. The PE shows staining with 11724-1-AP/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI. (10X).

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  IF Staining of human embronic stem cells using 11724-1-AP

  Confocal immunofluorescent analysis of human embronic stem cells with 11724-1-AP at dilution of 1:400. The PE shows staining with 11724-1-AP/PE. The DAPI shows nuclear staining by DAPI. The MERGE is the merge of PE and DAPI. (20X).

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  WB analysis of NCCIT using 11724-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 11724-1-AP (LIN28 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: NCCIT cell, NCCIT cells, K-562 cells, mouse embryo tissue
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human embronic stem cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IHC: See 18 publications below
• IF: See 8 publications below
• IP: See 1 publications below

Product Information

11724-1-AP targets LIN28 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: canine, human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LIN28 fusion protein Ag2312
• Full Name: lin-28 homolog (C. elegans)
• Calculated molecular weight: 209 aa, 23 kDa
• Observed molecular weight: 28 kDa
• GenBank accession number: BC028566
• Gene symbol: LIN28
• Gene ID (NCBI): 79727
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell[PMID: 20139967]. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna[PMID: 21057460]. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells[PMID: 22078496]. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against full length LIN28 of human origin. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.

Publications

Species	Application	Title
human	IHC	Clin Endocrinol (Oxf); Expression of LIN28 and its regulatory microRNAs in adult adrenocortical cancer.; Authors - André M Faria
human	WB	Cell Death Discov; ETV4 promotes breast cancer cell stemness by activating glycolysis and CXCR4-mediated sonic Hedgehog signaling.; Authors - Tao Zhu
	WB	Cell Rep; Non-microRNA binding competitively inhibits LIN28 regulation.; Authors - Frederick E Tan
human	IHC	Pediatr Dev Pathol; Immunohistochemical expression of embryonic stem cell markers in malignant rhabdoid tumors.; Authors - Deisch Jeremy J
human		Pediatr Surg Int; RNA-binding protein LIN28 is a sensitive marker of pediatric yolk sac tumors.; Authors - Shaoguang Feng
human	WB	Cell J; Rational Development of A Polycistronic Plasmid with A CpG-Free Bacterial Backbone as A Potential Tool for Direct Reprogramming.; Authors - Kianoush Dormiani

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