LIN28 Antibody 32 Publications

Rabbit Polyclonal| Catalog number: 11724-1-AP

Featured Product KD/KO validated

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Freight/Packing

Con: 47 μg/150 μl

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Species specificity:
human, mouse, rat, dog

Positive WB detected in:
NCCIT cell,K-562 cells, mouse embryo tissue, NCCIT cells

Positive IP detected in:
K-562 cells

Positive IHC detected in:
human prostate cancer tissue

Positive IF detected in:
human embronic stem cells

Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:50-1:200
IF : 1:20-1:200

Product Information


Source:
Rabbit

Purification method:
Antigen affinity purification

Isotype:
IgG

Storage:
PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information


Full name:
lin-28 homolog (C. elegans)

Calculated molecular weight:
209aa,23 kDa

Observed molecular weight:
28 kDa

GenBank accession number:

Gene ID (NCBI):

Gene symbol
LIN28

Synonyms
CSDD1, FLJ12457, LIN 28, lin 28 homolog (C. elegans), Lin 28A, LIN28, Lin28A, Protein lin 28 homolog A, ZCCHC1
Background

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell[PMID: 20139967]. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna[PMID: 21057460]. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells[PMID: 22078496]. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against full length LIN28 of human origin. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.


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