Validation Data Gallery
|Positive WB detected in||NCCIT cell, NCCIT cells, K-562 cells, mouse embryo tissue|
|Positive IP detected in||K-562 cells|
|Positive IHC detected in||human prostate cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||human embronic stem cells|
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:200|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
11724-1-AP targets LIN28 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||canine, human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||LIN28 fusion protein Ag2312|
|Full Name||lin-28 homolog (C. elegans)|
|Calculated molecular weight||209 aa, 23 kDa|
|Observed molecular weight||28 kDa|
|GenBank accession number||BC028566|
|Gene ID (NCBI)||79727|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell[PMID: 20139967]. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna[PMID: 21057460]. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells[PMID: 22078496]. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against full length LIN28 of human origin. The calculated molecular weight of LIN28 is 23 kDa, but the modified LIN28 is about 28 kDa.
Clin Endocrinol (Oxf)
Expression of LIN28 and its regulatory microRNAs in adult adrenocortical cancer.
Cell Death Discov
ETV4 promotes breast cancer cell stemness by activating glycolysis and CXCR4-mediated sonic Hedgehog signaling.
Non-microRNA binding competitively inhibits LIN28 regulation.
Pediatr Dev Pathol
Immunohistochemical expression of embryonic stem cell markers in malignant rhabdoid tumors.
Pediatr Surg Int
RNA-binding protein LIN28 is a sensitive marker of pediatric yolk sac tumors.
Rational Development of A Polycistronic Plasmid with A CpG-Free Bacterial Backbone as A Potential Tool for Direct Reprogramming.