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LMOD1 Polyclonal antibody

LMOD1 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15117-1-AP

Synonyms

1D, 64 kDa autoantigen 1D, 64 kDa autoantigen 1D3, 64 kDa autoantigen D1, 64kD, D1, Leiomodin 1, leiomodin 1 (smooth muscle), Leiomodin, muscle form, LMOD1, SM LMOD, Smooth muscle leiomodin



Tested Applications

Positive WB detected inmouse skeletal muscle tissue, mouse bladder tissue, mouse uterus tissue, HT-29 cells
Positive IP detected inmouse skeletal muscle tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:5000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15117-1-AP targets LMOD1 in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LMOD1 fusion protein Ag7176
Full Name leiomodin 1 (smooth muscle)
Calculated molecular weight 67 kDa
Observed molecular weight 85 kDa, 64 kDa
GenBank accession numberBC001755
Gene symbol LMOD1
Gene ID (NCBI) 25802
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LMOD1 was initially identified as a 64-kDa autoantigen in an expression screen with sera from patients with Hashimoto’s thyroiditis. Recently immunohistochemistry and immunoblotting of LMOD1 demonstrated its highly restrictive pattern of expression in SMC lineages,like adult aorta, and bladder. LMOD1 is also shown to exhibit an embryonic and adult expression pattern. These data identify LMOD1 as a new SMC-restricted gene associated with the SMC differentiated phenotype.

Protocols

Product Specific Protocols
WB protocol for LMOD1 antibody 15117-1-APDownload protocol
IHC protocol for LMOD1 antibody 15117-1-APDownload protocol
IF protocol for LMOD1 antibody 15117-1-APDownload protocol
IP protocol for LMOD1 antibody 15117-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Redox Biol

Vascular smooth muscle-MAPK14 is required for neointimal hyperplasia by suppressing VSMC differentiation and inducing proliferation and inflammation.

Authors - Wen Wu
humanIF

Pathogens

Putative Autoantigen Leiomodin-1 Is Expressed in the Human Brain and in the Membrane Fraction of Newly Formed Neurons.

Authors - David W Nauen
mouseWB,IHC

J Biol Chem

Leiomodin 1, a new serum response factor-dependent target gene expressed preferentially in differentiated smooth muscle cells.

Authors - Nanda Vivek V
humanWB

Arterioscler Thromb Vasc Biol

Identification and initial functional characterization of a human vascular cell-enriched long noncoding RNA.

Authors - Robert D Bell
mouseWB

Arterioscler Thromb Vasc Biol

CRISPR-Cas9 genome editing of a single regulatory element nearly abolishes target gene expression in mice--brief report.

Authors - Yu Han
mouseIF

J Neurosci

Gene Expression by Mouse Inner Ear Hair Cells during Development.

Authors - Déborah I Scheffer