LPHN1 Polyclonal antibody

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  IHC staining of mouse brain using 30142-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 30142-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 30142-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 30142-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 30142-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30142-1-AP (LPHN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse kidney using 30142-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using LPHN1 antibody (30142-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse kidney using 30142-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse kidney tissue using LPHN1 antibody (30142-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LPHN1 Polyclonal Antibody for IHC, IF-P, ELISA

Cat No. 30142-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, IF-P, ELISA

Synonyms

CIRL-1, CIRL1, CIRL 1, Calcium-independent alpha-latrotoxin receptor 1, Adhesion G protein-coupled receptor L1

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Tested Applications

• Positive IHC detected in: mouse brain tissue, rat kidney tissue, mouse kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: mouse kidney tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30142-1-AP targets LPHN1 in IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LPHN1 fusion protein Ag31093
• Full Name: latrophilin 1
• Calculated Molecular Weight: 163 kDa
• GenBank Accession Number: NM_001008701
• Gene Symbol: LPHN1
• Gene ID (NCBI): 22859
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94910
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Latrophilin-1 (LPHN1) was isolated as the main high-affinity receptor for α-latrotoxin from black widow spider venom, a powerful presynaptic secretagogue (PMID: 30967757). aGPCR latrophilin 1 (ADGRL1/LPHN1) is involved in controlling energy balance and food intake. Although this receptor is present in adipocytes and many neurons, where it is essential for axon growth cone formation and neuronal. It is present in Adipocytes and many neurons, where it is essential for Axon growth cone formation and neuronal function (PMID: 38664368).