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LRDD Antibody 0 Publications

Rabbit Polyclonal| Catalog number: 12119-1-AP

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Con: 45 μg/150 μl

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Tested Applications

Applications:
WB, IP, IHC, IF, ELISA
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Images See all

LRDD Antibody WB L02 cells 12119-1-AP

L02 cells were subjected to SDS PAGE followed by western blot with 12119-1-AP(LRDD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours

LRDD Antibody IP L02 cells 12119-1-AP

IP Result of anti-LRDD (IP:12119-1-AP, 4ug; Detection:12119-1-AP 1:500) with L02 cells lysate 2800ug.

LRDD Antibody IHC human pancreas tissue 12119-1-AP

Immunohistochemical of paraffin-embedded human pancreas using 12119-1-AP(LRDD antibody) at dilution of 1:50 (under 40x lens)

LRDD Antibody IF Hela cells 12119-1-AP

Immunofluorescent analysis of Hela cells, using LRDD antibody 12119-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Species specificity:
human, mouse, rat
Positive WB detected in:
L02 cells, HEK-293 cells
Positive IP detected in:
L02 cells
Positive IHC detected in:
human pancreas tissue, human liver tissue

Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected in:
Hela cells
Recommended dilution:
WB : 1:200-1:1000
IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
IHC : 1:20-1:200
IF : 1:10-1:100

Protocols

Product-specific protocols

Standard Protocols

Product Information

Source:
Rabbit
Purification method:
Antigen affinity purification
Isotype:
IgG
Storage:
PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. Aliquoting is unnecessary for -20oC storage.

Immunogen Information

Immunogen:
LRDD fusion protein Ag2761
Full name:
leucine-rich repeats and death domain containing
Calculated molecular weight:
893aa,98 kDa
Observed molecular weight:
55 kDa
GenBank accession number:
BC014904
Gene ID (NCBI):
55367
Gene symbol
LRDD
Synonyms
LRDD, PIDD

Background

P53-induced protein with a death domain (PIDD/LRDD) is a component of the PIDDosome. PIDD contains 910 residues with 7 leucine rich repeats (LRRs), 2 ZU-5 domains and a C-terminal death domain (DD). PIDD can be cleaved into shorter fragments generating a PIDD-N fragment of 48 kD (residues 1-446), a PIDD-C fragment of 51 kD (residues 447-910) and a PIDD-CC fragment of 37 kD (residues 589-910). Auto-cleavage of PIDD determines the downstream signaling events. The PIDD-C fragment mediates activation of NFκB via the recruitment of RIP1 and NEMO, while PIDD-CC causes caspase-2 activation, which leads to apoptosis.


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