LRP1 Polyclonal antibody, PBS Only

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  WB analysis using 26106-1-AP (same clone as 26106-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 26106-1-AP (LRP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26106-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse liver using 26106-1-AP (same clone as 26106-1-PBS)

  IP result of anti-LRP1 (IP:26106-1-AP, 4ug; Detection:26106-1-AP 1:300) with mouse liver tissue lysate 4000ug. This data was developed using the same antibody clone with 26106-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 26106-1-AP (same clone as 26106-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26106-1-AP (LRP1 antibody) at dilution of 1:200 (under 0x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 26106-1-PBS in a different storage buffer formulation.

LRP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 26106-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, ELISA

Synonyms

APR, Apolipoprotein E receptor, APOER, Alpha-2-macroglobulin receptor, Alpha 2 macroglobulin receptor

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Product Information

26106-1-PBS targets LRP1 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LRP1 fusion protein Ag23098
• Full Name: low density lipoprotein-related protein 1 (alpha-2-macroglobulin receptor)
• Calculated Molecular Weight: 505 kDa
• Observed Molecular Weight: 85-90 kDa
• GenBank Accession Number: BC045107
• Gene Symbol: LRP1
• Gene ID (NCBI): 4035
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q07954
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LRP1 (Prolow-density lipoprotein receptor-related protein 1), also known as A2MR, is a multifunctional cell surface receptor that interacts through its cytoplasmic tail with adaptor and scaffold proteins that participate in cellular signaling. It is synthesized in the endoplasmic reticulum as a transmembrane glycosylated precursor that migrates with an apparent molecular mass of about 600 kd on SDS-polyacrylamide gels. After it reaches the Golgi complex, the protein is cleaved to generate two subunits with apparent molecular masses of approximately 515 and 85 kd respectively (PMID: 2112085). It involved in cellular lipid homeostasis. Involved in the plasma clearance of chylomicron remnants and activated LRPAP1 (alpha 2-macroglobulin), as well as the local metabolism of complexes between plasminogen activators and their endogenous inhibitors. Acts as an LRPAP1 alpha-2-macroglobulin receptor. May modulate cellular events, such as APP metabolism, kinase-dependent intracellular signaling, neuronal calcium signaling as well as neurotransmission (PMID:12888553).