Validation Data Gallery
|Positive WB detected in
|MCF-7 cells, pig heart tissue, mouse liver tissue, mouse heart tissue, rat heart tissue
|Positive IHC detected in
|human liver tissue, mouse heart tissue, mouse brown adipose tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)
|WB : 1:2000-1:10000
|IHC : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
26951-1-AP targets Perilipin 5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, pig, mouse, rat samples.
|human, pig, mouse, rat
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|Perilipin 5 fusion protein Ag25272
|lipid storage droplet protein 5
|Calculated molecular weight
|463 aa, 51 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Perilipin 5 (also known as LSDP5/OXPAT) is a lipid droplet (LD) coat protein that promotes association of LDs with mitochondria and is mainly present in tissues with a high fat-oxidative capacity, such as heart, skeletal muscles and brown adipose tissue. It plays an important role in the regulation of cardiac lipid storage and function.
J Mol Cell Biol
Knockdown of hepatocyte Perilipin-3 mitigates hepatic steatosis and steatohepatitis caused by hepatocyte CGI-58 deletion in mice
miR-183 and miR-96 orchestrate both glucose and fat utilization in skeletal muscle.
Int J Mol Sci
Effects of Simvastatin on Lipid Metabolism in Wild-Type Mice and Mice with Muscle PGC-1α Overexpression.
Perilipin 5 protects against oxygen-glucose deprivation/reoxygenation-elicited neuronal damage by inhibiting oxidative stress and inflammatory injury via the Akt-GSK-3β-Nrf2 pathway.
Aryl hydrocarbon receptor promotes lipid droplet biogenesis and metabolic shift in respiratory Club cells.
Biochem Biophys Res Commun
Perilipin 5 ameliorates high-glucose-induced podocyte injury via Akt/GSK-3β/Nrf2-mediated suppression of apoptosis, oxidative stress, and inflammation.