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Perilipin 5 Polyclonal antibody

Perilipin 5 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, pig, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 26951-1-AP

Synonyms

LSDP5, OXPAT, Perilipin 5, PLIN5



Tested Applications

Positive WB detected inMCF-7 cells, pig heart tissue, mouse liver tissue, mouse heart tissue, rat heart tissue
Positive IHC detected inhuman liver tissue, mouse heart tissue, mouse brown adipose tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

26951-1-AP targets Perilipin 5 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, pig, mouse, rat samples.

Tested Reactivity human, pig, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Perilipin 5 fusion protein Ag25272
Full Name lipid storage droplet protein 5
Calculated molecular weight 463 aa, 51 kDa
Observed molecular weight 51-55 kDa
GenBank accession numberBC131524
Gene symbol LSDP5
Gene ID (NCBI) 440503
RRIDAB_2880699
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Perilipin 5 (also known as LSDP5/OXPAT) is a lipid droplet (LD) coat protein that promotes association of LDs with mitochondria and is mainly present in tissues with a high fat-oxidative capacity, such as heart, skeletal muscles and brown adipose tissue. It plays an important role in the regulation of cardiac lipid storage and function.

Protocols

Product Specific Protocols
WB protocol for Perilipin 5 antibody 26951-1-APDownload protocol
IHC protocol for Perilipin 5 antibody 26951-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

J Mol Cell Biol

Knockdown of hepatocyte Perilipin-3 mitigates hepatic steatosis and steatohepatitis caused by hepatocyte CGI-58 deletion in mice

Authors - Xinyu Bao
mouseWB

EMBO Rep

miR-183 and miR-96 orchestrate both glucose and fat utilization in skeletal muscle.

Authors - Hui Wang
mouseWB

Int J Mol Sci

Effects of Simvastatin on Lipid Metabolism in Wild-Type Mice and Mice with Muscle PGC-1α Overexpression.

Authors - Miljenko V Panajatovic
mouseWB

Int Immunopharmacol

Perilipin 5 protects against oxygen-glucose deprivation/reoxygenation-elicited neuronal damage by inhibiting oxidative stress and inflammatory injury via the Akt-GSK-3β-Nrf2 pathway.

Authors - Kang Huo
humanWB,IF

Hum Cell

Aryl hydrocarbon receptor promotes lipid droplet biogenesis and metabolic shift in respiratory Club cells.

Authors - Hsueh-Chun Wang
mouseWB

Biochem Biophys Res Commun

Perilipin 5 ameliorates high-glucose-induced podocyte injury via Akt/GSK-3β/Nrf2-mediated suppression of apoptosis, oxidative stress, and inflammation.

Authors - Jie Feng