ChromoTek Lamin-Chromobody® plasmid (TagGFP2)

DNA plasmid encoding for anti-Lamin VHH (anti-Lamin Nanobody) fused to TagGFP2.

Vector type

Mammalian expression vector

Promoter

PCMV IE

Reporter

TagGFP2

Epitope

Lamin

Publications

7

Cat no : lcg

Synonyms

Chromobody, Lamin Chromobody

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Validation Data Gallery

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  U2OS cell line expressing Lamin-Chromobody fused to the green fluorescent protein TagGFP

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Product Information

DNA plasmid encoding for anti-Lamin VHH (anti-Lamin Nanobody) fused to TagGFP2.

• Applications: Live cell imaging
• Specificity: Lamin A/C, tested in human cell lines (HeLa, U2OS) and insect S2 cells.
• Vector type: Mammalian expression vector
• Promoter: PCMV IE
• Reporter: TagGFP2
• Codon usage: Mammalian
• Selection: Kan/Neo
• Encoded Nanobody/ VHH: Monoclonal anti-Lamin single domain antibody (sdAb) fragment
• Sequence: With the Lamin-Chromobody plasmid you receive the sequence information of the Alpaca antibody to Lamin fused to TagGFP2, as well as the full vector sequence.
• Microscopy techniques: Wide-field epifluorescence microscopy; confocal microscopy
• Transfection: Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
• Storage Condition: Shipped at ambient temperature. Store at -20 °C

Publications

Application	Title
	Antibodies (Basel); A Strategy to Optimize the Generation of Stable Chromobody Cell Lines for Visualization and Quantification of Endogenous Proteins in Living Cells.; Authors - Bettina-Maria Keller
	Cell Mol Bioeng; Characterization of 3D Printed Stretching Devices for Imaging Force Transmission in Live-Cells.; Authors - Carl R Mayer
	Sci Rep; A novel epitope tagging system to visualize and monitor antigens in live cells with chromobodies.; Authors - Bjoern Traenkle
IF	J Cell Sci; The prophase oocyte nucleus is a homeostatic G-actin buffer.; Authors - Kathleen Scheffler
	PLoS Pathog; Fluorescent protein tagging of adenoviral proteins pV and pIX reveals 'late virion accumulation compartment'.; Authors - Søren Pfitzner
	Nat Commun; GPCR-induced calcium transients trigger nuclear actin assembly for chromatin dynamics.; Authors - Ying Wang