ChromoTek Lamin-Chromobody® plasmid (TagGFP2)

Validation Data Gallery

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  U2OS cell line expressing Lamin-Chromobody fused to the green fluorescent protein TagGFP

DNA plasmid encoding for anti-Lamin VHH (anti-Lamin Nanobody) fused to TagGFP2.

Cat No. lcg

Publications(9)

Specificity

Lamin

Applications

IF, Live cell imaging

Vector type

Mammalian expression vector

Reporter

TagGFP2

Synonyms

Chromobody, Lamin Chromobody

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Product Information

DNA plasmid encoding for anti-Lamin VHH (anti-Lamin Nanobody) fused to TagGFP2.

• Description: The Lamin-Chromobody visualizes the nuclear lamina.

  • Easily transfect your cells with the Lamin-Chromobody plasmid

  • Visualize the nuclear lamina without interfering with its cellular functions

  • Monitor the nuclear integrity and morphology during live cell microscopy

• Applications: Trace the nuclear lamina in live cells in real-time; Monitor the nuclear integrity and morphology; Use the Lamin-Chromobody® as a biosensor for real-time assays of mitosis or apoptosis
• Specificity/Target: Lamin A/C, tested in human cell lines (HeLa, U2OS) and insect S2 cells.
• Reporter: TagGFP2
• Vector type: Mammalian expression vector
• Promoter: Constitutive CMV IE
• Codon usage: Mammalian
• Selection: Kan/Neo
• Sequence: With the Lamin-Chromobody plasmid you receive the sequence information of the Alpaca antibody to Lamin fused to TagGFP2, as well as the full vector sequence.
• Transfection: Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
• Microscopy techniques: Wide-field epifluorescence microscopy; confocal microscopy
• Storage Condition: Shipped at ambient temperature. Store at -20 °C

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Publications

Application	Title
	Nat Cell Biol; A transient pool of nuclear F-actin at mitotic exit controls chromatin organization.; Authors - Christian Baarlink
	Nat Commun; GPCR-induced calcium transients trigger nuclear actin assembly for chromatin dynamics.; Authors - Ying Wang
	PLoS Pathog; Fluorescent protein tagging of adenoviral proteins pV and pIX reveals 'late virion accumulation compartment'.; Authors - Søren Pfitzner
IF	J Cell Sci; The prophase oocyte nucleus is a homeostatic G-actin buffer.; Authors - Kathleen Scheffler
	Sci Rep; A novel epitope tagging system to visualize and monitor antigens in live cells with chromobodies.; Authors - Bjoern Traenkle
	Cell Mol Bioeng; Characterization of 3D Printed Stretching Devices for Imaging Force Transmission in Live-Cells.; Authors - Carl R Mayer