ChromoTek Lamin-Chromobody® plasmid (TagGFP2)

DNA plasmid encoding for anti-Lamin VHH (anti-Lamin Nanobody) fused to TagGFP2.

Vector type

Mammalian expression vector

Promoter

PCMV IE

Reporter

TagGFP2

Epitope

Lamin

Cat no : lcg

Synonyms



Product Information

DNA plasmid encoding for anti-Lamin VHH (anti-Lamin Nanobody) fused to TagGFP2.

Applications Live cell imaging
SpecificityLamin A/C, tested in human cell lines (HeLa, U2OS) and insect S2 cells.
Vector typeMammalian expression vector
PromoterPCMV IE
ReporterTagGFP2
Codon usageMammalian
SelectionKan/Neo
Encoded Nanobody/ VHHMonoclonal anti-Lamin single domain antibody (sdAb) fragment
SequenceWith the Lamin-Chromobody plasmid you receive the sequence information of the Alpaca antibody to Lamin fused to TagGFP2, as well as the full vector sequence.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
StorageShipped at ambient temperature. Store at -20 °C

Publications

ApplicationTitle

Antibodies (Basel)

A Strategy to Optimize the Generation of Stable Chromobody Cell Lines for Visualization and Quantification of Endogenous Proteins in Living Cells.

Authors - Bettina-Maria Keller

Cell Mol Bioeng

Characterization of 3D Printed Stretching Devices for Imaging Force Transmission in Live-Cells.

Authors - Carl R Mayer

Sci Rep

A novel epitope tagging system to visualize and monitor antigens in live cells with chromobodies.

Authors - Bjoern Traenkle

PLoS Pathog

Fluorescent protein tagging of adenoviral proteins pV and pIX reveals 'late virion accumulation compartment'.

Authors - Søren Pfitzner

Nat Commun

GPCR-induced calcium transients trigger nuclear actin assembly for chromatin dynamics.

Authors - Ying Wang

Nat Cell Biol

A transient pool of nuclear F-actin at mitotic exit controls chromatin organization.

Authors - Christian Baarlink