Cat no : lcg
DNA plasmid encoding for anti-Lamin VHH (anti-Lamin Nanobody) fused to TagGFP2.
|Applications||Live cell imaging|
|Specificity||Lamin A/C, tested in human cell lines (HeLa, U2OS) and insect S2 cells.|
|Vector type||Mammalian expression vector|
|Encoded Nanobody/ VHH||Monoclonal anti-Lamin single domain antibody (sdAb) fragment|
|Sequence||With the Lamin-Chromobody plasmid you receive the sequence information of the Alpaca antibody to Lamin fused to TagGFP2, as well as the full vector sequence.|
|Microscopy techniques||Wide-field epifluorescence microscopy; confocal microscopy|
|Transfection||Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.|
|Storage Condition||Shipped at ambient temperature. Store at -20 °C|
A Strategy to Optimize the Generation of Stable Chromobody Cell Lines for Visualization and Quantification of Endogenous Proteins in Living Cells.
Cell Mol Bioeng
Characterization of 3D Printed Stretching Devices for Imaging Force Transmission in Live-Cells.
A novel epitope tagging system to visualize and monitor antigens in live cells with chromobodies.
J Cell Sci
The prophase oocyte nucleus is a homeostatic G-actin buffer.
Fluorescent protein tagging of adenoviral proteins pV and pIX reveals 'late virion accumulation compartment'.
GPCR-induced calcium transients trigger nuclear actin assembly for chromatin dynamics.