ChromoTek Lamin-Chromobody® plasmid (TagGFP2)
DNA plasmid encoding for anti-Lamin VHH (anti-Lamin Nanobody) fused to TagGFP2.
Vector type
Mammalian expression vector
Promoter
PCMV IE
Reporter
TagGFP2
Epitope
Lamin
Cat no : lcg
Synonyms
Validation Data Gallery
Product Information
DNA plasmid encoding for anti-Lamin VHH (anti-Lamin Nanobody) fused to TagGFP2.
| Applications | Live cell imaging |
| Specificity | Lamin A/C, tested in human cell lines (HeLa, U2OS) and insect S2 cells. |
| Vector type | Mammalian expression vector |
| Promoter | PCMV IE |
| Reporter | TagGFP2 |
| Codon usage | Mammalian |
| Selection | Kan/Neo |
| Encoded Nanobody/ VHH | Monoclonal anti-Lamin single domain antibody (sdAb) fragment |
| Sequence | With the Lamin-Chromobody plasmid you receive the sequence information of the Alpaca antibody to Lamin fused to TagGFP2, as well as the full vector sequence. |
| Microscopy techniques | Wide-field epifluorescence microscopy; confocal microscopy |
| Transfection | Transfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type. |
| Storage | Shipped at ambient temperature. Store at -20 °C |
Publications
| Application | Title |
|---|---|
Antibodies (Basel) A Strategy to Optimize the Generation of Stable Chromobody Cell Lines for Visualization and Quantification of Endogenous Proteins in Living Cells. | |
Cell Mol Bioeng Characterization of 3D Printed Stretching Devices for Imaging Force Transmission in Live-Cells. | |
Sci Rep A novel epitope tagging system to visualize and monitor antigens in live cells with chromobodies. | |
PLoS Pathog Fluorescent protein tagging of adenoviral proteins pV and pIX reveals 'late virion accumulation compartment'. | |
Nat Commun GPCR-induced calcium transients trigger nuclear actin assembly for chromatin dynamics. | |
Nat Cell Biol A transient pool of nuclear F-actin at mitotic exit controls chromatin organization. |