ChromoTek Lamin-Chromobody® plasmid (TagGFP2)

DNA plasmid encoding for anti-Lamin VHH (anti-Lamin Nanobody) fused to TagGFP2.

Specificity

Lamin

Applications

IF, Live cell imaging

Vector type

Mammalian expression vector

Reporter

TagGFP2

Cat no : lcg

Synonyms

Chromobody, Lamin Chromobody



Product Information

DNA plasmid encoding for anti-Lamin VHH (anti-Lamin Nanobody) fused to TagGFP2.

DescriptionThe Lamin-Chromobody visualizes the nuclear lamina.

• Easily transfect your cells with the Lamin-Chromobody plasmid

• Visualize the nuclear lamina without interfering with its cellular functions

• Monitor the nuclear integrity and morphology during live cell microscopy

• Stable fluorescent HeLa and U2OS cell lines ready to go for cellular screening

ApplicationsTrace the nuclear lamina in live cells in real-time
Monitor the nuclear integrity and morphology
Use the Lamin-Chromobody® as a biosensor for real-time assays of mitosis or apoptosis
Specificity/TargetLamin A/C, tested in human cell lines (HeLa, U2OS) and insect S2 cells.
ReporterTagGFP2
Vector typeMammalian expression vector
PromoterConstitutive CMV IE
Codon usageMammalian
SelectionKan/Neo
SequenceWith the Lamin-Chromobody plasmid you receive the sequence information of the Alpaca antibody to Lamin fused to TagGFP2, as well as the full vector sequence.
TransfectionTransfection of Chromobody plasmids into mammalian cells can be done with standard DNA-transfection methods, e.g. lipofection (Lipofectamine 2000® from Thermo Scientific), according to the manufacturer’s protocol for the transfection reagent. Please choose the transfection method that works the best for your cell type.
Microscopy techniquesWide-field epifluorescence microscopy; confocal microscopy
Storage ConditionShipped at ambient temperature. Store at -20 °C

Publications

ApplicationTitle

Nat Cell Biol

A transient pool of nuclear F-actin at mitotic exit controls chromatin organization.

Authors - Christian Baarlink

Nat Commun

GPCR-induced calcium transients trigger nuclear actin assembly for chromatin dynamics.

Authors - Ying Wang

PLoS Pathog

Fluorescent protein tagging of adenoviral proteins pV and pIX reveals 'late virion accumulation compartment'.

Authors - Søren Pfitzner
IF

J Cell Sci

The prophase oocyte nucleus is a homeostatic G-actin buffer.

Authors - Kathleen Scheffler

Sci Rep

A novel epitope tagging system to visualize and monitor antigens in live cells with chromobodies.

Authors - Bjoern Traenkle

Cell Mol Bioeng

Characterization of 3D Printed Stretching Devices for Imaging Force Transmission in Live-Cells.

Authors - Carl R Mayer