Validation Data Gallery
|Positive WB detected in||hESC cells, human brain tissue, human heart tissue, NCCIT cell, mouse liver tissue|
|Positive IHC detected in||human small intestine tissue, human ovary tissue, human heart tissue, human testis tissue, human spleen tissue, human lung tissue, human brain tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:200-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
16177-1-AP targets Lin28A-specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Full Name||lin-28 homolog (C. elegans)|
|Calculated molecular weight||23 kDa|
|Observed molecular weight||28 kDa|
|GenBank accession number||NM_024674|
|Gene ID (NCBI)||79727|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human LIN28.This antibody recognize Lin28A specifically.
Cell Death Differ
LIN28A enhances regenerative capacity of human somatic tissue stem cells via metabolic and mitochondrial reprogramming.
Activation of the Lin28/let-7 Axis by Loss of ESE3/EHF Promotes a Tumorigenic and Stem-like Phenotype in Prostate Cancer.
Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.
The human FOXM1 homolog promotes basal progenitor cell proliferation and cortical folding in mouse.
Cell Death Dis
Long noncoding RNA TUG1 facilitates osteogenic differentiation of periodontal ligament stem cells via interacting with Lin28A.
Hepatitis C virus-induced cancer stem cell-like signatures in cell culture and murine tumor xenografts.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Carolina (Verified Customer) (09-26-2019)
Antibody was more specific than the previous Lin28A antibody.
KUNHUA (Verified Customer) (02-01-2019)
Got a very strong band at 25-37kd with 1:1000 concentration.