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  • KD/KO Validated

Lin28A-specific Polyclonal antibody

Lin28A-specific Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 16177-1-AP

Synonyms

CSDD1, LIN 28, lin 28 homolog (C. elegans), Lin 28A, LIN28, Lin28A, Lin28A-specific, Protein lin 28 homolog A, ZCCHC1



Tested Applications

Positive WB detected inhESC cells, human brain tissue, human heart tissue, NCCIT cell, mouse liver tissue
Positive IHC detected inhuman small intestine tissue, human ovary tissue, human heart tissue, human testis tissue, human spleen tissue, human lung tissue, human brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

16177-1-AP targets Lin28A-specific in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name lin-28 homolog (C. elegans)
Calculated molecular weight 23 kDa
Observed molecular weight 28 kDa
GenBank accession numberNM_024674
Gene symbol LIN28
Gene ID (NCBI) 79727
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LIN28 is one of the four key human factors (OCT4, SOX2, NANOG and LIN28) used to reprogram human fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (Ips) cell. Lin28 is a marker of undifferentiated human embryonic stem cells and a cytoplasmic Mrna-binding protein that binds to and enhances the translation of the IGF2 Mrna. LIN28 has also been shown to bind to the let-7 pre-miRNA and block production of the mature let-7 microRNA in mouse embryonic stem cells. Affinity purified rabbit anti-LIN28 can be used to demonstrate pluripotency of ES and Ips cells, and to detect LIN28 transgene expression in the process of reprogramming. This antibody is a rabbit polyclonal antibody raised against a peptide mapping within human LIN28.This antibody recognize Lin28A specifically.

Protocols

Product Specific Protocols
WB protocol for Lin28A-specific antibody 16177-1-APDownload protocol
IHC protocol for Lin28A-specific antibody 16177-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell Death Dis

Lin28A promotes IRF6-regulated aerobic glycolysis in glioma cells by stabilizing SNHG14.

Authors - Jinjing Lu
human,mouse,ratWB,IF

FASEB J

Lin 28A/Occludin axis: An aberrantly activated pathway in intestinal epithelial cells leading to impaired barrier function under total parenteral nutrition.

Authors - Yang Liu
  • KD Validated
humanWB

J Virol

Hepatitis C virus-induced cancer stem cell-like signatures in cell culture and murine tumor xenografts.

Authors - Ali Naushad N
humanWB

Cell Death Dis

Long noncoding RNA TUG1 facilitates osteogenic differentiation of periodontal ligament stem cells via interacting with Lin28A.

Authors - Qin He
humanWB

EMBO Rep

Lin28 enhances de novo fatty acid synthesis to promote cancer progression via SREBP-1.

Authors - Yang Zhang
  • KD Validated
humanWB

Cancer Res

Activation of the Lin28/let-7 Axis by Loss of ESE3/EHF Promotes a Tumorigenic and Stem-like Phenotype in Prostate Cancer.

Authors - Domenico Albino

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Carolina (Verified Customer) (09-26-2019)

Antibody was more specific than the previous Lin28A antibody.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:20
  • Cell Tissue Type: Human Testis
FH

KUNHUA (Verified Customer) (02-01-2019)

Got a very strong band at 25-37kd with 1:1000 concentration.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: human erythroid progenitor