TIP47 Polyclonal antibody

TIP47 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 10694-1-AP

Synonyms

M6PRBP1, Perilipin 3, Placental protein 17, PLIN3, TIP47



Tested Applications

Positive WB detected in3T3-L1 cells, Jurkat cells, HEK-293 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inmouse liver tissue, mouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMEF cells, oleic acid treated HeLa cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2500
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

10694-1-AP targets TIP47 in WB, IP, IHC, IF, FC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TIP47 fusion protein Ag1028
Full Name mannose-6-phosphate receptor binding protein 1
Calculated molecular weight 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC007566
Gene symbol M6PRBP1
Gene ID (NCBI) 10226
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.

Protocols

Product Specific Protocols
WB protocol for TIP47 antibody 10694-1-APDownload protocol
IHC protocol for TIP47 antibody 10694-1-APDownload protocol
IF protocol for TIP47 antibody 10694-1-APDownload protocol
IP protocol for TIP47 antibody 10694-1-APDownload protocol
FC protocol for TIP47 antibody 10694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Cycle

Feedback activation of GATA1/miR-885-5p/PLIN3 pathway decreases sunitinib sensitivity in clear cell renal cell carcinoma.

Authors - Dayong Yao
mouseWB

Cell Rep

HILPDA Uncouples Lipid Droplet Accumulation in Adipose Tissue Macrophages from Inflammation and Metabolic Dysregulation.

Authors - Xanthe A M H van Dierendonck
mouse,humanWB,IHC

Cell Death Discov

Simultaneously targeting SOAT1 and CPT1A ameliorates hepatocellular carcinoma by disrupting lipid homeostasis.

Authors - Meiling Ren
humanWB

Hum Cell

Aryl hydrocarbon receptor promotes lipid droplet biogenesis and metabolic shift in respiratory Club cells.

Authors - Hsueh-Chun Wang
humanWB

PLoS One

HCV Core Residues Critical for Infectivity Are Also Involved in Core-NS5A Complex Formation.

Authors - Katarzyna Gawlik
humanWB,IF

Sci Rep

Delayed recruiting of TPD52 to lipid droplets - evidence for a "second wave" of lipid droplet-associated proteins that respond to altered lipid storage induced by Brefeldin A treatment.

Authors - Yuyan Chen