TIP47 Polyclonal antibody

TIP47 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

30

Reviews (1)

Cat no : 10694-1-AP

Synonyms

47 kDa MPR binding protein, Cargo selection protein TIP47, M6PRBP1, Perilipin 3, Placental protein 17, PLIN3, PP17, TIP47

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Validation Data Gallery

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  WB analysis of 3T3-L1 using 10694-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10694-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10694-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10694-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10694-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10694-1-AP (TIP47 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 10694-1-AP

  IP Result of anti-TIP47 (IP:10694-1-AP, 3ug; Detection:10694-1-AP 1:1000) with HeLa cells lysate 1000ug.

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  IHC staining of mouse liver using 10694-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 10694-1-AP (TIP47 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 10694-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 10694-1-AP (TIP47 antibody) at dilution of 1:50 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse ovary using 10694-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 10694-1-AP (TIP47 antibody) at dilution of 1:50 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MEF cells using 10694-1-AP

  IF result of anti-TIP47 (10694-1-AP,1:500) with MEF cell by Dr.Hector Alex Saka.

• 

  IF Staining of HeLa using 10694-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (10694-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). F-actin was stained with CL594-phalloidin.

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  IF Staining of HeLa using 10694-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed oleic acid treated HeLa cells using TIP47 antibody (10694-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  FC experiment of HeLa using 10694-1-AP

  1X10^6 HeLa cells were stained with 0.2ug TIP47 antibody (10694-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells, 3T3-L1 cells, HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: mouse liver tissue, mouse ovary tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: MEF cells, oleic acid treated HeLa cells
• Positive FC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 26 publications below
• IHC: See 3 publications below
• IF: See 6 publications below

Product Information

10694-1-AP targets TIP47 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: TIP47 fusion protein Ag1028
• Full Name: mannose-6-phosphate receptor binding protein 1
• Calculated molecular weight: 47 kDa
• Observed molecular weight: 47 kDa
• GenBank accession number: BC007566
• Gene symbol: M6PRBP1
• Gene ID (NCBI): 10226
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.

Publications

Species	Application	Title
mouse	WB	Cell Metab; AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.; Authors - Hui Luo
mouse	WB	Nat Commun; Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.; Authors - Fangjun Yu
human	IF	Nat Commun; The autophagy protein ATG9A enables lipid mobilization from lipid droplets.; Authors - Elodie Mailler
human	WB	Hepatology; CIDEC/Fsp27 is regulated by PPARα and plays a critical role in fasting- and diet-induced hepatosteatosis.; Authors - Cédric Langhi
mouse	WB	Nat Commun; O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity.; Authors - Yunfan Yang
human	IF	J Cell Biol; A role for ubiquitin ligases and Spartin/SPG20 in lipid droplet turnover.; Authors - Eastman Scott W SW

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Christine (Verified Customer) (09-19-2023)

Tested on HEK293T cells stimulated by 300 uM oleic acid overnight to stimulate the formation of lipid droplets. This antibody (tested only at 1:100 so far) decorates the lipid droplets, it worked well.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HEK293T

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