Validation Data Gallery
|Positive WB detected in||HEK-293 cells, Jurkat cells, 3T3-L1 cells, HeLa cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||mouse liver tissue, mouse ovary tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||MEF cells, oleic acid treated HeLa cells|
|Positive FC detected in||HeLa cells|
|Western Blot (WB)||WB : 1:2000-1:10000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
10694-1-AP targets TIP47 in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||TIP47 fusion protein Ag1028|
|Full Name||mannose-6-phosphate receptor binding protein 1|
|Calculated molecular weight||47 kDa|
|Observed molecular weight||47 kDa|
|GenBank accession number||BC007566|
|Gene ID (NCBI)||10226|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Mannose 6-phosphate receptors (M6PRs) transport newly synthesized lysosomal hydrolases from the Golgi to prelysosomes and then return to the Golgi for another round of transport. M6PRBP1 (mannose-6-phosphate receptor binding protein 1), also known as TIP47, PLIN3 or PP17, interacts with the cytoplasmic domains of both cation-independent and cation-dependent M6PRs, and is required for endosome-to-Golgi transport. In addition to M6PR recycling, M6PRBP1 plays a role in lipid droplet biogenesis, and is also implicated in rhodopsin photobleaching and viral infection. M6PRBP1 has been found to be expressed in a variety of human tissues (including colon, liver and lung parenchyme, mammary gland, and skin) and is overexpressed in certain cancer cell lines. It binds to lipid droplets and also occurs in cytosol and on endosomal membranes.
|Product Specific Protocols|
|WB protocol for TIP47 antibody 10694-1-AP||Download protocol|
|IHC protocol for TIP47 antibody 10694-1-AP||Download protocol|
|IF protocol for TIP47 antibody 10694-1-AP||Download protocol|
|IP protocol for TIP47 antibody 10694-1-AP||Download protocol|
|Click here to view our Standard Protocols|
AIDA Selectively Mediates Downregulation of Fat Synthesis Enzymes by ERAD to Retard Intestinal Fat Absorption and Prevent Obesity.
Deficiency of intestinal Bmal1 prevents obesity induced by high-fat feeding.
The autophagy protein ATG9A enables lipid mobilization from lipid droplets.
CIDEC/Fsp27 is regulated by PPARα and plays a critical role in fasting- and diet-induced hepatosteatosis.
O-GlcNAc transferase inhibits visceral fat lipolysis and promotes diet-induced obesity.
J Cell Biol
A role for ubiquitin ligases and Spartin/SPG20 in lipid droplet turnover.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Christine (Verified Customer) (09-19-2023)
Tested on HEK293T cells stimulated by 300 uM oleic acid overnight to stimulate the formation of lipid droplets. This antibody (tested only at 1:100 so far) decorates the lipid droplets, it worked well.