MAF Polyclonal antibody

MAF Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 55013-1-AP

Synonyms

c MAF, cMAF, MAF, Proto oncogene c Maf, Transcription factor Maf



Tested Applications

Positive WB detected inA431 cells, multi-cells/tissue
Positive IP detected inA431 cells
Positive IF detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

55013-1-AP targets MAF in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian)
Calculated molecular weight 42 kDa
Observed molecular weight 48-50 kDa
GenBank accession numberNM_005360
Gene symbol MAF
Gene ID (NCBI) 4094
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

MAF, also named as c-Maf, belongs to the bZIP family and Maf subfamily. MAF acts as a transcriptional activator or repressor. It is involved in embryonic lens fiber cell development. MAF increases T cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, it transactivates strongly the glucagon gene promoter through the G1 element. MAF activates transcription of the CD13 proximal promoter in endothelial cells. It is involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. When overexpressed, MAF represses anti-oxidant reponse element (ARE)-mediated transcription. It is involved either as an oncogene or as a tumor suppressor, depending on the cell context. A chromosomal aberration involving MAF is found in some forms of multiple myeloma (MM). Defects in MAF are the cause of cataract pulverulent juvenile-onset MAF-related (CAPJOM). Defects in MAF are the cause of cataract congenital cerulean type 4 (CCA4). The antibody is specific to MAF. And it could recognise the 50 kDa band that also be detected in the paper (PMID: 25770584 ) .

Protocols

Product Specific Protocols
WB protocol for MAF antibody 55013-1-APDownload protocol
IF protocol for MAF antibody 55013-1-APDownload protocol
IP protocol for MAF antibody 55013-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IP

Cell Death Dis

Anti-bacterial and anti-viral nanchangmycin displays anti-myeloma activity by targeting Otub1 and c-Maf.

Authors - Yujia Xu
humanWB

Acta Pharmacol Sin

Mebendazole elicits potent antimyeloma activity by inhibiting the USP5/c-Maf axis.

Authors - Xue-Han Chen
humanIF

Graefes Arch Clin Exp Ophthalmol

The novel mutation P36R in LRP5L contributes to congenital membranous cataract via inhibition of laminin γ1 and c-MAF.

Authors - Liyao Sun
humanWB

J Biol Chem

The deubiquitinase USP7 stabilizes Maf proteins to promote myeloma cell survival.

Authors - Yuanming He
humanWB

Cell Commun Signal

Inhibition of the Otub1/c-Maf axis by the herbal acevaltrate induces myeloma cell apoptosis.

Authors - Tong Sun
mouseWB

J Immunol

Malat1 Suppresses Immunity to Infection through Promoting Expression of Maf and IL-10 in Th Cells.

Authors - James P Hewitson

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

H (Verified Customer) (04-05-2020)

This protein has a very short half life, and present in the nucleus. I could see a clear band after the nuclear-cytosolic fractionation.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: HEK293T
MAF Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:55013-1-AP)