Tested Applications
| Positive WB detected in | A431 cells, A375 cells, HeLa cells, HepG2 cells, K-562 cells |
| Positive IP detected in | A431 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55013-1-AP targets c-MAF in WB, IF/ICC, IP, ELISA, CUT&RUN applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian) |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42-52 kDa |
| GenBank Accession Number | NM_005360 |
| Gene Symbol | MAF |
| Gene ID (NCBI) | 4094 |
| RRID | AB_10863127 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75444 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAF, also named as c-Maf, belongs to the bZIP family and Maf subfamily. MAF acts as a transcriptional activator or repressor. It is involved in embryonic lens fiber cell development. MAF increases T cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, it transactivates strongly the glucagon gene promoter through the G1 element. MAF activates transcription of the CD13 proximal promoter in endothelial cells. It is involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. When overexpressed, MAF represses anti-oxidant reponse element (ARE)-mediated transcription. It is involved either as an oncogene or as a tumor suppressor, depending on the cell context. A chromosomal aberration involving MAF is found in some forms of multiple myeloma (MM). Defects in MAF are the cause of cataract pulverulent juvenile-onset MAF-related (CAPJOM). Defects in MAF are the cause of cataract congenital cerulean type 4 (CCA4). The antibody is specific to MAF. And it could recognise the 50 kDa band that also be detected in the paper (PMID: 25770584 ) .
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for c-MAF antibody 55013-1-AP | Download protocol |
| IP protocol for c-MAF antibody 55013-1-AP | Download protocol |
| WB protocol for c-MAF antibody 55013-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-c-Maf antibody (Cat# 55013-1-AP) has 18 citations published across 17 journals including Immunity, Nature Neuroscience, Cell Death & Disease, Journal of Biological Chemistry, Journal of Immunology, Frontiers in Immunology, and Cell Signaling. Research fields span immunology, oncology (particularly multiple myeloma), neuroscience, pharmacology, and cell biology, with applications primarily in Western blot and immunofluorescence.
| Species | Application | Title |
|---|---|---|
Immunity RAS P21 Protein Activator 3 (RASA3) Specifically Promotes Pathogenic T Helper 17 Cell Generation by Repressing T-Helper-2-Cell-Biased Programs. | ||
Nat Neurosci Spinal cord Tau pathology induces tactile deficits and cognitive impairment in Alzheimer's disease via dysregulation of CCK neurons
| ||
Cell Death Dis Anti-bacterial and anti-viral nanchangmycin displays anti-myeloma activity by targeting Otub1 and c-Maf. | ||
Cell Commun Signal Inhibition of the Otub1/c-Maf axis by the herbal acevaltrate induces myeloma cell apoptosis. | ||
Acta Pharmacol Sin Mebendazole elicits potent antimyeloma activity by inhibiting the USP5/c-Maf axis. | ||
Environ Pollut Aluminum promotes B1 cells to produce IL-10 and impairs adaptive immune system |
Reviews
What customers say
Both reviewers used this antibody for Western Blot at a 1:1000 dilution and reported positive results. One user (5/5) confirmed it works well for WB and has been used in a publication with primary CD4 T cells. The other (4/5) noted the target protein has a very short half-life and is nuclear-localized, but still observed a clear band after nuclear-cytosolic fractionation in HEK293T cells. Overall, users find it reliable for detecting this protein by Western Blot.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Katie (Verified Customer) (04-08-2022) | Works well for western blot. We have published with this antibody
|
H (Verified Customer) (04-05-2020) | This protein has a very short half life, and present in the nucleus. I could see a clear band after the nuclear-cytosolic fractionation.
 |
