MAF Polyclonal antibody
MAF Polyclonal Antibody for IF, IP, WB,ELISA
Host / Isotype
Rabbit / IgG
human, mouse, rat
WB, IP, IF, ELISA
Cat no : 55013-1-AP
Validation Data Gallery
|Positive WB detected in||A431 cells, multi-cells/tissue|
|Positive IP detected in||A431 cells|
|Positive IF detected in||A431 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
|WB||See 8 publications below|
|IF||See 1 publications below|
|IP||See 1 publications below|
55013-1-AP targets MAF in WB, IP, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Full Name||v-maf musculoaponeurotic fibrosarcoma oncogene homolog (avian)|
|Calculated molecular weight||42 kDa|
|Observed molecular weight||48-50 kDa|
|GenBank accession number||NM_005360|
|Gene ID (NCBI)||4094|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
MAF, also named as c-Maf, belongs to the bZIP family and Maf subfamily. MAF acts as a transcriptional activator or repressor. It is involved in embryonic lens fiber cell development. MAF increases T cell susceptibility to apoptosis by interacting with MYB and decreasing BCL2 expression. Together with PAX6, it transactivates strongly the glucagon gene promoter through the G1 element. MAF activates transcription of the CD13 proximal promoter in endothelial cells. It is involved in the initial chondrocyte terminal differentiation and the disappearance of hypertrophic chondrocytes during endochondral bone development. When overexpressed, MAF represses anti-oxidant reponse element (ARE)-mediated transcription. It is involved either as an oncogene or as a tumor suppressor, depending on the cell context. A chromosomal aberration involving MAF is found in some forms of multiple myeloma (MM). Defects in MAF are the cause of cataract pulverulent juvenile-onset MAF-related (CAPJOM). Defects in MAF are the cause of cataract congenital cerulean type 4 (CCA4). The antibody is specific to MAF. And it could recognise the 50 kDa band that also be detected in the paper (PMID: 25770584 ) .
|Product Specific Protocols|
|WB protocol for MAF antibody 55013-1-AP||Download protocol|
|IF protocol for MAF antibody 55013-1-AP||Download protocol|
|IP protocol for MAF antibody 55013-1-AP||Download protocol|
|Click here to view our Standard Protocols|
RAS P21 Protein Activator 3 (RASA3) Specifically Promotes Pathogenic T Helper 17 Cell Generation by Repressing T-Helper-2-Cell-Biased Programs.
Molecular Mechanisms Driving IL-10- Producing B Cells Functions: STAT3 and c-MAF as Underestimated Central Key Regulators?
Malat1 Suppresses Immunity to Infection through Promoting Expression of Maf and IL-10 in Th Cells.
Cell Commun Signal
Inhibition of the Otub1/c-Maf axis by the herbal acevaltrate induces myeloma cell apoptosis.
J Biol Chem
The deubiquitinase USP7 stabilizes Maf proteins to promote myeloma cell survival.
Graefes Arch Clin Exp Ophthalmol
The novel mutation P36R in LRP5L contributes to congenital membranous cataract via inhibition of laminin γ1 and c-MAF.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Katie (Verified Customer) (04-08-2022)
Works well for western blot. We have published with this antibody
H (Verified Customer) (04-05-2020)
This protein has a very short half life, and present in the nucleus. I could see a clear band after the nuclear-cytosolic fractionation.