MAOA Polyclonal antibody

• Featured Product
• KD/KO Validated

MAOA Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

26

Cat no : 10539-1-AP

Synonyms

monoamine oxidase A, MAO-A, MAO A, EC:1.4.3.21, Amine oxidase [flavin-containing] A

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Validation Data Gallery

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  WB analysis of LNCaP using 10539-1-AP

  LNCaP cells were subjected to SDS PAGE followed by western blot with 10539-1-AP (MAOA antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 10539-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10539-1-AP (MAOA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 10539-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 10539-1-AP (MAOA antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 10539-1-AP

  IP result of anti-MAOA (IP:10539-1-AP, 3ug; Detection:10539-1-AP 1:600) with human placenta tissue lysate 2800ug.

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  IHC staining of human pancreas cancer using 10539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 10539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10539-1-AP (MAOA antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas using 10539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 10539-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human kidney using 10539-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 10539-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver using 10539-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 10539-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 10539-1-AP (MAOA antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: LNCaP cells, human placenta tissue
• Positive IP detected in: human placenta tissue
• Positive IHC detected in: human pancreas cancer tissue, human kidney tissue, human liver tissue, human pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 21 publications below
• IHC: See 9 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Product Information

10539-1-AP targets MAOA in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAOA fusion protein Ag0821
• Full Name: monoamine oxidase A
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC008064
• Gene Symbol: MAOA
• Gene ID (NCBI): 4128
• RRID: AB_2137251
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAOA belongs to the flavin monoamine oxidase family. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and has important functions in the metabolism of neuroactive and vasoactive amines in the central nervous system and peripheral tissues. MAOA preferentially oxidizes biogenic amines such as 5-hydroxytryptamine (5-HT), norepinephrine and epinephrine.

Publications

Species	Application	Title
mouse	IF	Cell; In situ identification of cellular drug targets in mammalian tissue.; Authors - Zhengyuan Pang
human	WB	Sci Adv; p53 loss activates prometastatic secretory vesicle biogenesis in the Golgi.; Authors - Xiaochao Tan
	WB	Sci Adv; Global ubiquitylation analysis of mitochondria in primary neurons identifies endogenous Parkin targets following activation of PINK1.; Authors - Odetta Antico
		J Clin Invest; A pro-tumorigenic secretory pathway activated by p53 deficiency in lung adenocarcinoma.; Authors - Xiaochao Tan
human	WB,IHC	J Hepatol; Monoamine oxidase A suppresses hepatocellular carcinoma metastasis by inhibiting the adrenergic system and its transactivation of EGFR signaling.; Authors - Jun Li
mouse	WB	Aging (Albany NY); A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.; Authors - Xiao Wu